Non-Human Primate Core

开发有效的艾滋病疫苗仍然是艾滋病毒研究的最优先事项之一。研究 对艾滋病毒发病机制、疫苗设计和抗逆转录病毒治疗的研究需要使用适当的动物模型。然而，目前使用的SIV/恒河猴(RM)模型有严重的局限性。HIVRAD建议的总体目标是识别和表征新的、分子定义的、生理上相关的SIVsmm菌株，这些菌株可以重复地导致RMS的生产性感染，并更真实地概括当前流行的HIV-1毒株的遗传多样性和临床结果。这些新的挑战毒株也将被用来提供对粘膜HIV/SIV传播的新的机制洞察。位于匹兹堡大学的非人灵长类核心将负责管理大部分 本计划项目所需的灵长类研究。具体目标包括： 1.选择、监测和管理项目1和2要求的所有非人类灵长类动物。 目标是为这些项目提供适合拟议研究的动物，确保高质量的动物住房和一般饲养，并执行动物临床管理的方方面面，包括护理、治疗和长期监测。 2.协调HIVRAD内非人类灵长类研究的设计和安排 财团。目标是协调实验设计，安排有效的调度，收集、处理、存储和盘点RMS的所有样本，并将样本分发给研究小组。 3.进行项目1和2所需的所有尸检。目标是安排组织 采集、处理和储存，从血液、非淋巴和淋巴组织中分离单个核细胞，并进行所有组织病理学检查。 动物核心部分的目的是提供高质量的动物护理和住房，执行研究方案，标本收集和处理，尸检，以及获取和管理必要的动物临床和病理数据，以满足计划研究目标。潘德里亚博士和核心团队将与项目负责人和他们的实验室工作人员密切互动，以确保及时完成所有与NHP相关的研究目标。

The development of an effective AIDS vaccine remains one of the highest priorities in HIV research. Studies of HIV pathogenesis, vaccine design and antiretroviral treatments require the use of an appropriate animal model. However, the currently used SIV/rhesus macaque (RM) model has serious limitations. The overall objective of this HIVRAD proposal is identify and characterize novel, moleculariy defined, physiologically relevant SIVsmm strains that reproducibly lead to productive infection in RMs and more faithfully recapitulate the genetic diversity and the clinical outcome of currently circulating HIV-1 strains. These new challenge strains will also be used to provide new mechanistic insight into mucosal HIV/SIV transmission. The Nonhuman Primate Core located at the University of Pittsburgh will be responsible for managing a large portion of the primate studies required for this Program Projects. The Specific Aims include: 1. To select, monitor and manage all non-human primates required by Projects 1 and 2. The goals are to provide these projects with the appropriate animals for the proposed studies, to ensure high quality animal housing and general husbandry, and to perform all aspects of the clinical management of the animals, including the care, treatment and long-term monitoring. 2. To coordinate the design and scheduling of non-human primate studies within the HIVRAD consortium. The goals are to coordinate the experimental design, arrange for efficient scheduling, collect, process, store and inventory all samples from RMs, and distribute the samples to the research groups. 3. To perform all necropsies required by Projects 1 and 2. The goals are to arrange for tissue collection, processing and storage, perform mononuclear cell separation from blood, non-lymphoid and lymphoid tissues and conduct all histopathological examinations. The purpose of the Animal Core Component is to provide high quality of animal care and housing, execution of study protocols, specimen collection and processing, performance of necropsies, and acquisition and management of animal clinical and pathological data necessary to meet the program research objectives. Dr. Pandrea and the Core team will closely interact with Project leaders and their laboratory staff to ensure timely completion of all NHP related research goals.