Non-Human Primate Core

研制有效的艾滋病疫苗仍然是艾滋病毒研究的最高优先事项之一。研究 艾滋病病毒的发病机制，疫苗设计和抗逆转录病毒治疗需要使用适当的动物模型。然而，目前使用的SIV/恒河猴（RM）模型有严重的局限性。本HIVRAD提案的总体目标是鉴定和表征新的、分子学定义的、生理学相关的SIVsmm毒株，这些毒株可重复地导致RM中的生产性感染，并更忠实地再现目前流行的HIV-1毒株的遗传多样性和临床结果。这些新的挑战菌株也将用于提供对粘膜HIV/SIV传播的新的机制见解。位于匹兹堡大学的非人类灵长类核心将负责管理大部分 本计划项目所需的灵长类动物研究。具体目标包括： 1.选择、监测和管理项目1和2所需的所有非人灵长类动物。的 目标是为这些项目提供用于拟议研究的适当动物，确保高质量的动物饲养和一般饲养，并执行动物临床管理的所有方面，包括护理，治疗和长期监测。 2.协调HIVRAD内非人灵长类动物研究的设计和时间安排 财团其目标是协调实验设计，安排有效的时间表，收集，处理，存储和库存所有样品从RM，并分发样品到研究小组。 3.进行项目1和2要求的所有尸检。我们的目标是安排组织 采集、处理和储存，从血液、非淋巴和淋巴组织中分离单核细胞，并进行所有组织病理学检查。 动物核心组件的目的是提供高质量的动物护理和圈养、研究方案的执行、标本采集和处理、尸检的执行以及满足项目研究目标所需的动物临床和病理学数据的采集和管理。Pandrea博士和核心团队将与项目负责人及其实验室工作人员密切互动，以确保及时完成所有与NHP相关的研究目标。

The development of an effective AIDS vaccine remains one of the highest priorities in HIV research. Studies of HIV pathogenesis, vaccine design and antiretroviral treatments require the use of an appropriate animal model. However, the currently used SIV/rhesus macaque (RM) model has serious limitations. The overall objective of this HIVRAD proposal is identify and characterize novel, moleculariy defined, physiologically relevant SIVsmm strains that reproducibly lead to productive infection in RMs and more faithfully recapitulate the genetic diversity and the clinical outcome of currently circulating HIV-1 strains. These new challenge strains will also be used to provide new mechanistic insight into mucosal HIV/SIV transmission. The Nonhuman Primate Core located at the University of Pittsburgh will be responsible for managing a large portion of the primate studies required for this Program Projects. The Specific Aims include: 1. To select, monitor and manage all non-human primates required by Projects 1 and 2. The goals are to provide these projects with the appropriate animals for the proposed studies, to ensure high quality animal housing and general husbandry, and to perform all aspects of the clinical management of the animals, including the care, treatment and long-term monitoring. 2. To coordinate the design and scheduling of non-human primate studies within the HIVRAD consortium. The goals are to coordinate the experimental design, arrange for efficient scheduling, collect, process, store and inventory all samples from RMs, and distribute the samples to the research groups. 3. To perform all necropsies required by Projects 1 and 2. The goals are to arrange for tissue collection, processing and storage, perform mononuclear cell separation from blood, non-lymphoid and lymphoid tissues and conduct all histopathological examinations. The purpose of the Animal Core Component is to provide high quality of animal care and housing, execution of study protocols, specimen collection and processing, performance of necropsies, and acquisition and management of animal clinical and pathological data necessary to meet the program research objectives. Dr. Pandrea and the Core team will closely interact with Project leaders and their laboratory staff to ensure timely completion of all NHP related research goals.