Golgi Studies of Schizophrenia

精神分裂症的高尔基体研究

• 批准号: 8448242
• 负责人: ANDREW J DWORK
• 金额: $ 48.63万
• 依托单位: NEW YORK STATE PSYCHIATRIC INSTITUTE DBA RESEARCH FOUNDATION FOR MENTAL HYGIENE, INC
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8448242
• 关键词: Administrative Supplement; Affect; Age; Amygdaloid structure; Animal Model; Apical; Atrophic; Autopsy; Brain; Brain region; Cells; Clinical; Cognitive deficits; Collection; Cytoskeletal Proteins; Data; Dendrites; Dendritic Spines; Diffusion weighted imaging; Dorsal; Forensic Medicine; Fresh Tissue; Functional disorder; Funding; Glutamates; Golgi Apparatus; Golgi method; Grant; Hippocampal Formation; Hippocampus (Brain); Horns; Human; Institutes; Institution; Laboratories; Lead; Left; MFGE8 gene; Macedonia; Macedonia (Republic); Medial; Medial Dorsal Nucleus; Mental disorders; Methods; Microtubule-Associated Protein 2; Midbrain structure; Modification; Morphology; Neurobiology; Neurons; Pattern; Population; Prefrontal Cortex; Procedures; Process; Pyramidal Cells; Random Allocation; Reporting; Sampling; Schizophrenia; Site; Slide; Specimen; Staining method; Stains; Stress; Structure; Techniques; Thalamic structure; Tissue Banks; Tissues; Toxicology; United States National Institutes of Health; Vertebral column; abstracting; brain tissue; cholinergic; density; dentate gyrus; hippocampal pyramidal neuron; human tissue; immunoreactivity; improved; in vivo; lateral ventricle; neurogenesis; neuronal cell body; neuropathology; prospective; psychologic; sex; success; tissue fixing; white matter

Abstract Golgi staining reveals the anatomical structure of dendrites. While Golgi methods have yielded some of the most robust histological findings in schizophrenia, studies that use these techniques are uncommon, probably because the techniques have been difficult to apply and notoriously capricious. We have developed a new modification of the Golgi-Cox method that has yielded unprecedented reliability, with consistent staining in over 100 consecutive blocks from over 70 consecutive autopsy cases. There is a uniform distribution of stained neurons that allows systematic random sampling of neurons for analysis. We propose to use this material to study dendritic arborization and spine density in 20 schizophrenia subjects matched by age and sex to 40 subjects without psychiatric disease, all evaluated clinically by psychological autopsy. We will analyze dendritic arborization and spine density in dorsal (BA9) and ventral (BA45) prefrontal cortex, and in the hippocampal formation (subiculum, CA3, CA1, and dentate gyrus). Since every section cut from a block is stained well, we propose to share the slides with other laboratories that wish to perform additional analyses. This application also includes continuation of our ongoing collection of brain specimens and psychological autopsies in the Republic of Macedonia. This collection provides the fresh tissue for our Golgi studies and for many other studies of the neurobiology of psychiatric illnesses, both in our department and at other institutions. The specimens and data are stored in our department and subjected to rigorous clinical, neuropathological, and toxicological review.

摘要 高尔基体染色显示树突的解剖结构。虽然高尔基体方法 已经在精神分裂症中产生了一些最有力的组织学发现， 使用这些技术是不常见的，可能是因为这些技术已经被 很难应用而且是出了名的反复无常我们开发了一种新的 Golgi-Cox方法产生了前所未有的可靠性，染色一致 在超过100个连续街区的70多个连续尸检案例中。有一个 染色神经元的均匀分布，允许系统随机采样 神经元分析我们建议使用这种材料来研究树突状树枝化， 20例精神分裂症患者与40例正常人脊柱密度比较 无精神疾病，均经心理尸检进行临床评估。我们将 分析背侧（BA 9）和腹侧（BA 45）的树突分支和棘密度 前额叶皮层和海马结构（下托、CA 3、CA 1和齿状回）中的神经元 脑回）。由于从块切下的每个切片都染色良好，我们建议共享 与希望进行额外分析的其他实验室合作。 这项申请还包括继续我们正在进行的收集大脑 在马其顿共和国的标本和心理解剖。此集合 为我们的高尔基体研究和许多其他研究提供了新鲜的组织。 神经生物学的精神疾病，无论是在我们的部门和其他机构。 标本和数据保存在我们的部门，并经过严格的临床， 神经病理学和毒理学综述。

项目成果

The contribution of HIV infection to intracranial arterial remodeling: a pilot study.

• DOI: 10.1111/j.1440-1789.2012.01358.x
• 发表时间: 2013-06
• 期刊: Neuropathology : official journal of the Japanese Society of Neuropathology
• 作者: Gutierrez J;Elkind MS;Petito C;Chung DY;Dwork AJ;Marshall RS
• 通讯作者: Marshall RS

Psychiatric brain collection in Macedonia: general lessons for scientific collaboration among countries of differing wealth.

马其顿的精神病学大脑收集：不同财富国家之间科学合作的一般教训。

• 发表时间: 2013
• 期刊: Prilozi (Makedonska akademija na naukite i umetnostite. Oddelenie za medicinski nauki)
• 作者: Rosoklija,G;Duma,A;Dwork,AJ
• 通讯作者: Dwork,AJ

Hippocampal angiogenesis and progenitor cell proliferation are increased with antidepressant use in major depression.

• DOI: 10.1016/j.biopsych.2012.04.024
• 发表时间: 2012-10-01
• 期刊: BIOLOGICAL PSYCHIATRY
• 影响因子: 10.6
• 作者: Boldrini, Maura;Hen, Rene;Underwood, Mark D.;Rosoklija, Gorazd B.;Dwork, Andrew J.;Mann, J. John;Arango, Victoria
• 通讯作者: Arango, Victoria