Development of a diagnostic for latent TB

潜伏性结核病诊断方法的开发

• 批准号: 8535548
• 负责人: FREDERICK D QUINN
• 金额: $ 20万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-17 至 2015-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8535548
• 关键词: Development; diagnostic; latent; TB

The Institute of Medicine report, "Ending Neglect: The Elimination of Tuberculosis in the United States," calls for the development of better tools for accurately identifying latently infected individuals. In countries such as the United States, where the incidence of latent tuberculosis is relatively low, the specificity of the current tuberculin skin test is too low to yield a high positive power of prediction. The goal of the proposed research is proof of concept for a diagnostic test of very high specificity, based upon the ultra sensitive detection of a Mycobacterium tuberculosis (Mtb) protein in peripheral blood. The specific aims are: to develop a bead capture ELISA sensitive enough to detect the Mtb homolog of alpha-crystallin at a low picomolar concentration, and to demonstrate that this protein can be detected in sera from Mtb - infected subjects. If this very specific and sensitive diagnostic test can be developed and commercialized, it could be used to guide prophylactic treatment recommendations for individuals latently infected with Mtb. Such a test has been identified as a high priority goal to facilitate a National plan to eliminate tuberculosis in the United States.

医学研究所的报告《结束忽视：消除美国的结核病》 要求开发更好的工具来准确识别潜伏感染的病毒 个人。在美国等国家，潜在结核病的发病率是 相对较低，目前的结核菌素皮肤试验的特异性太低，无法产生高阳性结果 预测的力量。 拟议研究的目标是对非常高的诊断测试进行概念验证 特异性，基于对结核分枝杆菌(Mtb)的超灵敏检测 外周血液中的蛋白质。本研究的具体目标是：研制一种敏感的微珠捕捉法 足以在较低的皮摩尔浓度下检测到α-晶体蛋白的Mtb同系物，并 证明在结核分枝杆菌感染者的血清中可以检测到这种蛋白。 如果这种非常特异和敏感的诊断测试能够被开发和商业化，它可能会 用于指导对潜伏感染的患者的预防治疗建议 山地车。这样的测试已被确定为促进国家计划的高度优先目标 在美国消灭结核病。