Regulation of akinete differentiation in Nostoc punctiforme

发菜Akinite分化的调控

基本信息

批准号：
8500371
负责人：
MICHAEL L SUMMERS
金额：
$ 17.1万
依托单位：
CALIFORNIA STATE UNIVERSITY NORTHRIDGE
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-01 至 2015-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8500371
关键词：
Address Affect Area Bacteria Biochemical Biochemistry Biological Models Biomedical Research Cell Differentiation process Cells Communities Complementary DNA Cyanobacterium DNA DNA Microarray Chip DNA-Directed RNA Polymerase Desiccation Development Developmental Biology Developmental Process Environment Event Family Filament Funding Future Gene Expression Regulation Gene Targeting Genes Genetic Genetic Transcription Genetic screening method Genome Germination Goals Grant Harvest Homologous Gene Hybrids In Vitro Knowledge Membrane Methods Microarray Analysis Microbiology Molecular Biology Molecular Genetics Mutagenesis Mutation Nature Nitrogen Nostoc Organism Pattern Phenotype Physiological Plasmids Prokaryotic Cells Proteins Public Health Publications RNA Regulation Regulator Genes Regulon Relative (related person)Reporter Reproduction spores Research Resistance Running Science Sequence Analysis Sigma Factor Speed Stress Students System Techniques Technology Testing Time Training Transcription Initiation Transcriptional Regulation Underrepresented Minority Water Work career cell type cost experience gene replacement genetic regulatory protein genome sequencing in vivo insight killings member mutant novel pathogenic bacteria programs promoter protein-histidine kinase public health relevance research study response screening skills success tool toxic bloom transcription factor

项目摘要

DESCRIPTION (provided by applicant): Nostoc punctiforme is a filamentous cyanobacterium capable of differentiation into motile hormogonia, nitrogen-fixing heterocysts, and spore-like dormant cells called akinetes. It serves as a model system for studying development due to its one-dimensional body plan, sequenced genome, facile genetic system, and a working DNA microarray. This study focuses on identifying and characterizing genes involved with akinete development. Akinetes can withstand environmental stress, such as desiccation and cold that kill normal vegetative cells within the filament. The first specific aim is to 1) identify members of the regulon controlled by an ECF sigma factor involved in akinete formation, and 2) to determine if its activity is controlled by an associated anti-sigma factor. Specific aim 2 will exhaustively test genes encoding putative DNA regulatory proteins that were identified in a previous DNA microarray for 1) akinete-specific expression using GFP reporters, and 2) akinete-involvement through the use of mutant analysis. Defective mutants in akinete formation or function will be further characterized genetically and phenotypically. Identification of genes involved in akinete formation will likely give insights into possibly novel mechanisms by which bacteria gain resistance to environmental stress. Additionally, this work will involve underrepresented students at CSU Northridge who will be trained using cutting-edge technology applicable to many aspects of the biomedical sciences. PUBLIC HEALTH RELEVANCE: Nostoc punctiforme is a simple model system used to study cellular differentiation from vegetative cells into dormant akinete cells that are resistant to environmental extremes. Knowledge of regulatory events essential for akinete formation will aid in understanding how more pathogenic bacteria gain resistance to environmental stress. Related cyanobacteria cause toxic blooms in water systems and persist in the environment as akinetes. Understanding akinete formation may identify targets to inhibit their formation or germination, thereby reducing survival and the extent of subsequent toxic blooms by toxic cyanobacteria.

描述（由申请人提供）：Nostoc Punctiforme是一种丝状蓝细菌，能够分化为运动荷尔蒙病，氮固定杂环和称为Akinetes的孢子样细胞。由于其一维身体计划，测序的基因组，易于遗传系统和工作DNA微阵列，它是研究发展的模型系统。这项研究重点是识别和表征与Akinete发育有关的基因。 Akinetes可以承受环境压力，例如干燥和寒冷，可杀死细丝中正常的营养细胞。第一个具体目的是：1）确定由Akinete形成的ECF Sigma因子控制的调节成员，以及2）确定其活性是否由相关的抗Sigma因子控制。具体的目标2将详尽测试编码假定的DNA调节蛋白的基因，这些基因在先前的DNA微阵列中鉴定出1）使用GFP报告基因的Akineete特异性表达，以及2）通过使用突变分析，使用Akinete-InkineTe-InsineTe-Insignets-Involvolt。在遗传和表型上将进一步表征Akinete形成或功能中的有缺陷的突变体。鉴定涉及阿基内特形成的基因可能会深入了解细菌对环境应激的抗性的新机制。此外，这项工作将涉及CSU Northridge的代表性不足的学生，他们将使用适用于生物医学科学许多方面的尖端技术进行培训。公共卫生相关性：Nostoc Punctiforme是一种简单的模型系统，用于研究从营养细胞到对环境极端具有抗性的休眠性ackinete细胞的细胞分化。对阿基内特形成必不可少的调节事件的了解将有助于了解更多的致病细菌如何抵抗环境压力。相关的蓝细菌会导致水系统中的有毒花朵，并在环境中持续存在为弧形。了解Akinete的形成可能会识别靶标抑制其形成或发芽的靶标，从而降低生存率以及随后的毒性蓝细菌的毒性开花程度。