MOLECULAR DETERMINANTS OF GEMCITABINE (Franklin Hays)

全身化疗是肿瘤学家治疗不可切除的非小细胞肺癌(NSCLC)的剩余途径之一。不幸的是，NSCLC表明，抗癌治疗不能提供显著的患者生存益处，而且有毒副作用。这些药物将以很小的幅度延长转移性疾病的进展时间，但对降低非小细胞肺癌死亡率几乎没有作用。当前提议的一个基本主题是，通过对药物处置和反应的更详细的分子理解，人们可以在非小细胞肺癌化疗中获得显着的改善。为了获得这一理解，我们将使用结构生物学和功能分析相结合的方法来研究一种与正常细胞和肿瘤细胞中吉西他滨摄取直接相关的人完整膜蛋白。平衡核苷转运体(Hent)家族在人体组织中广泛分布，可转运多种核苷类抗代谢产物，与化疗疗效直接相关。我们在这项建议中寻求解决的具体目标包括(1)在原子分辨率下确定hent的结构，(2)使用纯化的蛋白开发体外功能分析，以及(3)使用三个人肺癌细胞系来表征hent的功能决定因素。这些研究的结果将被调整，以确定治疗药物OCUS的调节和运输的分子基础。广泛的愿景是，这些新的分子洞察力可以推进到临床结果，指导非小细胞肺癌核苷类药物治疗的治疗决策。

Systemic chemotherapy is one of the remaining avenues available to an oncologist treating unresectable non-small cell lung cancer (NSCLC). Unfortunately, NSCLC indicated anticancer therapeutics do not provide significant patient survival benefits and have toxic side effects. These drugs will extend the time-to-progression of metastatic disease by small margins yet do little to decrease NSCLC mortality. An underlying theme to the current proposal is that one can obtain significant improvements in NSCLC chemotherapy with a more detailed molecular understanding of drug disposition and response. To obtain this understanding we will use a combination of structural biology and functional assays to study a human integral membrane protein directly linked to gemcitabine uptake within normal and neoplastic cells. The equilibrative nucleoside transporter (hENT) family is broadly distributed in human tissues, is known to transport a wide array of nucleoside antimetabolites, and is directly linked to chemotherapeutic outcomes. Specific goals we seek to address in this proposal include (1) determine the structure of hENT at atomic resolution, (2) develop in vitro functional assays using purified protein, and (3) characterize hENT functional determinants using three human lung cancer cell lines. Outcomes from these studies will be tuned to determine the molecular basis by which hENT regulatoin and transport of therapeutics occus. The broad vision is that these novel molecular insights can be advanced to clinical outcomes that guide treatment decisions in nucleoside based drug treatment of NSCLC.