Modified nucleotide cofactors and the interaction of the RNAome with the proteome

描述（由申请人提供）：我们有初步证据表明siRNA和miRNA大小的小RNA与修饰的核苷酸如辅酶A和bis-MGD偶联。这些修饰将允许小RNA与蛋白质组共价接合以在这些蛋白质上构成核酸条形码。我们建议使用强大的核酸杂交和选择技术来纯化共价连接到RNA的蛋白质，并使用质谱来识别这些连接的蛋白质。使用这些蛋白质的RNAi，我们将调查小RNA功能的缺陷，从而这些蛋白质参与小RNA的功能。因为所有的修饰都被预测发生在小RNA的5'端，所以这些修饰的RNA中的大多数将被调查系统地遗漏，其中大多数调查需要RNA上的5' OH或5'磷酸用于克隆。

DESCRIPTION (provided by applicant): We have preliminary evidence that small RNAs of the size of siRNA and miRNAs are coupled to modified nucleotides such as coenzyme A and bis-MGD. These modifications would allow small RNAs to covalently engage with the proteome to constitute nucleic acid bar codes on these proteins. We propose to use powerful nucleic acid hybridization and selection techniques to purify the proteins covalently linked to RNA and to use mass spectroscopy to identify those linked proteins. Using RNAi of these proteins we will survey for defects in small RNA function and thus an involvement of these proteins in the functions of small RNAs. Because all of the modifications are predicted to occur at the 5' end of small RNAs, most of these modified RNAs will have been systematically missed by surveys most of which demands 5' OH or 5' phosphates on the RNAs for cloning.