Quantifying Risk Factors for Nanoparticle Contact with UVB Exposed Skin

描述（由申请人提供）：工程纳米材料（NM）在消费品和技术应用中的扩大使用已经刺激了环境和消费者健康与安全（H&S）问题。本项目研究NM皮肤暴露的问题。我们采用一种系统的方法来量化暴露于急性和慢性紫外线辐射水平下NM的组成、电荷、尺寸对皮肤穿透的影响。在纳米颗粒穿透皮肤的条件下，我们将描述它们与表皮细胞（包括抗原提呈细胞）的关联，以建立其作为全身运输机制。我们将确定NM是否以及如何影响UVB诱导的免疫抑制和过敏性接触超敏反应。最后，我们将确定测量皮肤从内到外的水分流失是否是评估由外到内纳米渗透风险的可行方法。该项目将产生一个简明的工作体系，有助于评估与无意皮肤接触NM相关的风险因素，并发现NM可能影响皮肤免疫功能的新机制。

DESCRIPTION (provided by applicant): The expanding use of engineered nanomaterials (NM) in consumer products and technological applications has spurred environmental and consumer health and safety (H&S) concerns. This project investigates the question of NM skin exposure. We take a systematic approach to quantify effect on NM composition, charge, size on penetration through skin exposed to acute and chronic levels of ultraviolet radiation. Under conditions that NM penetrate skin we will characterize their association with epidermal cells, including antigen presenting cells to establish this as a mechanism of systemic transport. We will determine if and how NM may impact UVB induced immunosuppression and allergic contact hypersensitivity. Finally, we will determine if measuring inside-out water loss from skin is a viable means to assess outside-in NM penetration risk. This project will produce a concise body of work that will aid in assessing risk factors associated with unintentional NM skin exposure and discover novel mechanisms by which NM may impact skin immune function. PUBLIC HEALTH RELEVANCE: The expanding use of engineered nanomaterials (NM) in consumer products and technological applications has spurred environmental and consumer health and safety (H&S) concerns. This project investigates the question of NM contact on ultraviolet radiation exposed skin.