Reagents to Enhance Detection of Raw and Biologically Transformed Nanomaterials

增强原始纳米材料和生物转化纳米材料检测的试剂

基本信息

  • 批准号:
    8325876
  • 负责人:
  • 金额:
    $ 21.78万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2011
  • 资助国家:
    美国
  • 起止时间:
    2011-09-01 至 2014-08-31
  • 项目状态:
    已结题

项目摘要

The increasing use of engineered nanomaterials in industrial and consumer applications has raised serious concerns about their environment health safety (EH&S). A particular concern is for occupational exposure to raw materials in the form of highly dispersed spherical and fiber- like shapes. Researchers seek to quantify nanomaterial interactions with biological systems and to develop predictive models of nanoparticle EH&S risk. However, limitations imposed by the model systems employed, the lack of NP standards, the failure of researchers to quantify or report the physiochemical properties of the NP investigated, and the inadequate availability of sensitive analytical instrumentation, have collectively hampered progress in attaining these EH&S goals. In the proposed project we seek to overcome limitations in detecting nanoparticles by developing a set of reagents (NProbes) that will enhance their presence in biological tissues that due to their size may be obscured from detection by other means. NProbe reagents will function in a similar fashion to those used to detect and quantify proteins in standard immunohistological analysis. NProbes with high binding affinity to both raw NP and transformed nanomaterials will be generated using protein-based (antibody and fibronectin) phage display technology. Nanomaterials of interest include carbon nanotubes, metal oxides, metals, and semiconductors. We will validate the binding of NProbes to raw nanomaterials and test their cross reactivity to samples of related and dissimilar composition. NProbe binding to nanomaterials transformed by penetration through skin will be also be demonstrated. We believe that NProbe reagents will be useful to researchers assessing nanoparticles tissue interactions in all mammalian, vertebrate, invertebrates systems as well as detection of nanoparticles in the environment. Successfully development of NProbe reagents will have a transformative effect on nanoparticle risk assessment research.
工程纳米材料在工业和消费应用中的使用越来越多, 对环境健康安全(EH&S)提出了严重关切。特别令人关切的是 对于职业暴露于高度分散的球形和纤维形式的原材料, 比如形状研究人员试图量化纳米材料与生物系统的相互作用, 开发纳米颗粒EH&S风险的预测模型。然而,《公约》所规定的限制 采用的模型系统,缺乏NP标准,研究人员未能量化或 报告所研究的NP的理化性质,以及 敏感的分析仪器,共同阻碍了实现这些目标的进展 EH&S目标。在拟议的项目中,我们试图克服检测纳米粒子的局限性 通过开发一套试剂(NProbes), 由于它们的尺寸可能被其他装置遮挡而无法检测。NProbe试剂将 以类似于用于检测和定量标准中蛋白质的方式起作用。 免疫组织化学分析。对原始NP和转化的NP具有高结合亲和力的NProbe 纳米材料将使用基于蛋白质(抗体和纤连蛋白)的噬菌体展示产生 技术.感兴趣的纳米材料包括碳纳米管、金属氧化物、金属和纳米材料。 半导体我们将验证NProbes与原始纳米材料的结合,并测试其 与相关和不相似组成的样品的交叉反应性。N探针结合 纳米材料透过皮肤的转化也将得到演示。我们 我相信NProbe试剂将对研究人员评估纳米颗粒组织有用, 在所有哺乳动物、脊椎动物、无脊椎动物系统中的相互作用以及 环境中的纳米粒子。NProbe试剂的成功开发将为 对纳米颗粒风险评估研究的变革性影响。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Development and characterization of antibody reagents for detecting nanoparticles.
用于检测纳米颗粒的抗体试剂的开发和表征。
  • DOI:
    10.1039/c5nr04882f
  • 发表时间:
    2015-12-21
  • 期刊:
  • 影响因子:
    6.7
  • 作者:
    Ravichandran S;Sullivan MA;Callahan LM;Bentley KL;DeLouise LA
  • 通讯作者:
    DeLouise LA
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Lisa A DeLouise其他文献

Lisa A DeLouise的其他文献

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{{ truncateString('Lisa A DeLouise', 18)}}的其他基金

Mechanisms of Engineered Nanomaterial Modulation of Skin Immune Responses
工程纳米材料调节皮肤免疫反应的机制
  • 批准号:
    10308081
  • 财政年份:
    2012
  • 资助金额:
    $ 21.78万
  • 项目类别:
Quantifying Risk Factors for Nanoparticle Contact with UVB Exposed Skin
量化纳米颗粒接触 UVB 暴露皮肤的风险因素
  • 批准号:
    8481550
  • 财政年份:
    2012
  • 资助金额:
    $ 21.78万
  • 项目类别:
Mechanisms of Engineered Nanomaterial Modulation of Skin Immune Responses
工程纳米材料调节皮肤免疫反应的机制
  • 批准号:
    10059244
  • 财政年份:
    2012
  • 资助金额:
    $ 21.78万
  • 项目类别:
Quantifying Risk Factors for Nanoparticle Contact with UVB Exposed Skin
量化纳米颗粒接触 UVB 暴露皮肤的风险因素
  • 批准号:
    8272859
  • 财政年份:
    2012
  • 资助金额:
    $ 21.78万
  • 项目类别:
Reagents to Enhance Detection of Raw and Biologically Transformed Nanomaterials
增强原始纳米材料和生物转化纳米材料检测的试剂
  • 批准号:
    8030077
  • 财政年份:
    2011
  • 资助金额:
    $ 21.78万
  • 项目类别:
Porous Silicon Optical Sensor for Candida Detection
用于念珠菌检测的多孔硅光学传感器
  • 批准号:
    7062417
  • 财政年份:
    2005
  • 资助金额:
    $ 21.78万
  • 项目类别:
Porous Silicon Optical Sensor for Candida Detection
用于念珠菌检测的多孔硅光学传感器
  • 批准号:
    7619147
  • 财政年份:
    2005
  • 资助金额:
    $ 21.78万
  • 项目类别:
Porous Silicon Optical Sensor for Candida Detection
用于念珠菌检测的多孔硅光学传感器
  • 批准号:
    7225248
  • 财政年份:
    2005
  • 资助金额:
    $ 21.78万
  • 项目类别:
Porous Silicon Optical Sensor for Candida Detection
用于念珠菌检测的多孔硅光学传感器
  • 批准号:
    6924304
  • 财政年份:
    2005
  • 资助金额:
    $ 21.78万
  • 项目类别:
Porous Silicon Optical Sensor for Candida Detection
用于念珠菌检测的多孔硅光学传感器
  • 批准号:
    7407461
  • 财政年份:
    2005
  • 资助金额:
    $ 21.78万
  • 项目类别:

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