Basic Science II Core

基础科学核心II：动物模型、行为测试和组织学 基础科学核心II(BSCII)将专注于基础神经科学的活体和翻译方面，并将包括动物模型及其行为测试、组织学、显微镜和神经病理学。BSCII将协助研究人员进行动物模型和行为测试，使他们能够克服开发和表征新的啮齿动物模型的挑战，特别是行为表型，这可能超出他们的专业领域。核心将提供目前可用的最精细的神经艾滋病模型，包括艾滋病毒脑病的免疫缺陷小鼠模型，可诱导和组织特异性转基因、基因敲除或敲除模型，细胞移植模型，病毒载体传递等。还将提供学习和记忆认知变化以及周围神经疾病的行为测试方面的专业知识。在神经模型的培育和表型鉴定以及可能特别具有挑战性的行为测试方面的经验将是一种宝贵的资源。还将提供人体尸检的组织学和免疫组织化学评估方面的援助 以及来自包括艾滋病、PML、神经退行性疾病和中枢神经系统肿瘤在内的神经系统疾病患者的临床活检样本、从实验动物模型中获得的组织和细胞培养。该核心将提供神经病理评估、研究设计和数据解释，以及显微服务，包括组织样本的激光捕获显微解剖(LCM)接入和用于下游分子生物学应用的核酸提取，以及用于高分辨率显微分析的细胞和组织的双光子(2P)激发显微镜。这些服务将协助神经艾滋病社区的研究人员进行组织病理学分析和翻译研究。该核心还将为调查人员及其工作人员提供必要的培训，以获得独立执行任何例行核心职能所需的熟练程度。

BASIC SCIENCE CORE II: ANIMAL MODEL, BEHAVIORAL TESTING, AND HISTOLOGY Basic Science Core II (BSCII) will focus on in vivo and translational aspects of basic neuroscience and will encompass animal models and their behavioral testing, histology, microscopy, and neuropathology. The BSCII will assist investigators with animal models and behavioral testing allowing them to overcome the challenges of developing and characterizing new rodent models, particularly with behavioral phenotypes, which may be outside their areas of expertise. The core will offer the most refined models of NeuroAIDS currently available including immunodeficient mouse models of HIV encephalopathy, inducible and tissue specific transgenic, knockdown, or knockout models, cell transplantation models, viral vector delivery, etc. Expertise in behavioral testing for cognitive changes in learning and memory as well as peripheral neuropathies will be provided. The experience available in breeding and phenotyping of neurological models, and behavioral testing which can be particularly challenging, will be an invaluable resource. Assistance will also be provided with histological and immunohistochemical evaluation of human autopsy and biopsy clinical samples from patients with neurological disorders including AIDS, PML, neurodegenerative diseases, and CNS neoplasia, tissues harvested from experimental animal models, and cell cultures. The core will provide neuropathological evaluation, study design and data interpretation, and microscopy services including Laser Capture Microdissection (LCM) accessioning of tissue samples and nucleic acid extraction for downstream molecular biological applications and two-photon (2P) excitation microscopy of cells and tissues for high resolution microscopic analysis. These services will assist investigators in the neuroAIDS community with histopatholgical analysis and translational studies. The core will also provide the necessary training to the investigators and their staff to gain the proficiency needed to independently conduct any of the routine core functions.