Basic Science II Core

基础科学 II 核心

基本信息

批准号：
8668160
负责人：
Jennifer Gordon
金额：
$ 35.33万
依托单位：
TEMPLE UNIV OF THE COMMONWEALTH
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8668160
关键词：
AIDS neuropathy Acquired Immunodeficiency Syndrome Aging Animal Model Animals Area Autopsy Basic Science Behavioral Biological Biopsy Biopsy Specimen Brain Diseases Breeding Cell Culture Techniques Cell Transplantation Cells Clinical Communities Data Development Diagnosis Drug Delivery Systems Educational Status Evaluation Evaluation Studies Experimental Animal Model Genotype HIV Encephalopathy Histology Human In Vitro Injection of therapeutic agent Knock-out Knockout Mice Learning Logistics Memory Microscopic Microscopy Modeling Molecular Motor Mus Neoplasms Nervous System Physiology Neurodegenerative Disorders Neurologic Neurological Models Neurosciences Nucleic Acids Pathogenesis Patients Peripheral Nervous System Diseases Phenotype Prevention Procedures Research Design Research Personnel Resolution Resources Rodent Model Sampling Sensory Services Staining method Stains Therapeutic Tissue Harvesting Tissue Sample Tissues Training Transgenic Mice Transgenic Organisms Validation Viral Proteins Viral Vector animal tissue behavior test clinical Diagnosis cognitive change cognitive function design and construction diagnosis evaluation disease diagnosis experience immunodeficient mouse model improved in vivo investigator training laser capture microdissection model design mouse model nervous system disorder neuromuscular neuropathology translational study two-photon

项目摘要

BASIC SCIENCE CORE II: ANIMAL MODEL, BEHAVIORAL TESTING, AND HISTOLOGY Basic Science Core II (BSCII) will focus on in vivo and translational aspects of basic neuroscience and will encompass animal models and their behavioral testing, histology, microscopy, and neuropathology. The BSCII will assist investigators with animal models and behavioral testing allowing them to overcome the challenges of developing and characterizing new rodent models, particularly with behavioral phenotypes, which may be outside their areas of expertise. The core will offer the most refined models of NeuroAIDS currently available including immunodeficient mouse models of HIV encephalopathy, inducible and tissue specific transgenic, knockdown, or knockout models, cell transplantation models, viral vector delivery, etc. Expertise in behavioral testing for cognitive changes in learning and memory as well as peripheral neuropathies will be provided. The experience available in breeding and phenotyping of neurological models, and behavioral testing which can be particularly challenging, will be an invaluable resource. Assistance will also be provided with histological and immunohistochemical evaluation of human autopsy and biopsy clinical samples from patients with neurological disorders including AIDS, PML, neurodegenerative diseases, and CNS neoplasia, tissues harvested from experimental animal models, and cell cultures. The core will provide neuropathological evaluation, study design and data interpretation, and microscopy services including Laser Capture Microdissection (LCM) accessioning of tissue samples and nucleic acid extraction for downstream molecular biological applications and two-photon (2P) excitation microscopy of cells and tissues for high resolution microscopic analysis. These services will assist investigators in the neuroAIDS community with histopatholgical analysis and translational studies. The core will also provide the necessary training to the investigators and their staff to gain the proficiency needed to independently conduct any of the routine core functions.

基础科学核心II：动物模型，行为测试和组织学基础科学核心II（BSCII）将重点关注基本神经科学的体内和翻译方面，并将涵盖动物模型及其行为测试，组织学，显微镜和神经病理学。 BSCII将协助研究人员进行动物模型和行为测试，使他们能够克服开发和表征新的啮齿动物模型的挑战，尤其是在行为表型中，这可能超出了其专业知识领域。核心将提供目前可用的最精致的神经辅助模型，包括HIV脑病，诱导和组织特异性转基因，敲低或敲除模型的免疫缺陷的小鼠模型，细胞移植模型，病毒载体的传递等。在学习和记忆和记忆和记忆和膜病神经疗法方面的行为测试方面的专业知识。神经系统模型的繁殖和表型以及可能特别具有挑战性的行为测试的经验将是宝贵的资源。还将为人类尸检的组织学和免疫组织化学评估提供帮助以及来自包括艾滋病，PML，神经退行性疾病和中枢神经系统肿瘤的神经系统疾病患者的活检临床样本，从实验动物模型和细胞培养物中收获的组织。该核心将提供神经病理学评估，研究设计和数据解释以及显微镜服务，包括激光捕获显微解剖（LCM）组织样品的登录和核酸提取，用于下游分子生物学应用以及两光子（2p）的激发（2p）激发显微镜，用于高分辨率显微镜分析的细胞和组织。这些服务将帮助神经助长社区的调查人员进行组织造理分析和翻译研究。核心还将为调查人员及其员工提供必要的培训，以获得独立执行任何常规核心功能所需的熟练程度。