Basic Science II Core

基础科学 II 核心

• 批准号: 8668160
• 负责人: Jennifer Gordon
• 金额: $ 35.33万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8668160
• 关键词: AIDS neuropathy; Acquired Immunodeficiency Syndrome; Aging; Animal Model; Animals; Area; Autopsy; Basic Science; Behavioral; Biological; Biopsy; Biopsy Specimen; Brain Diseases; Breeding; Cell Culture Techniques; Cell Transplantation; Cells; Clinical; Communities; Data; Development; Diagnosis; Drug Delivery Systems; Educational Status; Evaluation; Evaluation Studies; Experimental Animal Model; Genotype; HIV Encephalopathy; Histology; Human; In Vitro; Injection of therapeutic agent; Knock-out; Knockout Mice; Learning; Logistics; Memory; Microscopic; Microscopy; Modeling; Molecular; Motor; Mus; Neoplasms; Nervous System Physiology; Neurodegenerative Disorders; Neurologic; Neurological Models; Neurosciences; Nucleic Acids; Pathogenesis; Patients; Peripheral Nervous System Diseases; Phenotype; Prevention; Procedures; Research Design; Research Personnel; Resolution; Resources; Rodent Model; Sampling; Sensory; Services; Staining method; Stains; Therapeutic; Tissue Harvesting; Tissue Sample; Tissues; Training; Transgenic Mice; Transgenic Organisms; Validation; Viral Proteins; Viral Vector; animal tissue; behavior test; clinical Diagnosis; cognitive change; cognitive function; design and construction; diagnosis evaluation; disease diagnosis; experience; immunodeficient mouse model; improved; in vivo; investigator training; laser capture microdissection; model design; mouse model; nervous system disorder; neuromuscular; neuropathology; translational study; two-photon

BASIC SCIENCE CORE II: ANIMAL MODEL, BEHAVIORAL TESTING, AND HISTOLOGY Basic Science Core II (BSCII) will focus on in vivo and translational aspects of basic neuroscience and will encompass animal models and their behavioral testing, histology, microscopy, and neuropathology. The BSCII will assist investigators with animal models and behavioral testing allowing them to overcome the challenges of developing and characterizing new rodent models, particularly with behavioral phenotypes, which may be outside their areas of expertise. The core will offer the most refined models of NeuroAIDS currently available including immunodeficient mouse models of HIV encephalopathy, inducible and tissue specific transgenic, knockdown, or knockout models, cell transplantation models, viral vector delivery, etc. Expertise in behavioral testing for cognitive changes in learning and memory as well as peripheral neuropathies will be provided. The experience available in breeding and phenotyping of neurological models, and behavioral testing which can be particularly challenging, will be an invaluable resource. Assistance will also be provided with histological and immunohistochemical evaluation of human autopsy and biopsy clinical samples from patients with neurological disorders including AIDS, PML, neurodegenerative diseases, and CNS neoplasia, tissues harvested from experimental animal models, and cell cultures. The core will provide neuropathological evaluation, study design and data interpretation, and microscopy services including Laser Capture Microdissection (LCM) accessioning of tissue samples and nucleic acid extraction for downstream molecular biological applications and two-photon (2P) excitation microscopy of cells and tissues for high resolution microscopic analysis. These services will assist investigators in the neuroAIDS community with histopatholgical analysis and translational studies. The core will also provide the necessary training to the investigators and their staff to gain the proficiency needed to independently conduct any of the routine core functions.

基础科学核心II：动物模型、行为测试和组织学 基础科学核心II（BSCII）将专注于基础神经科学的体内和转化方面，并将包括动物模型及其行为测试，组织学，显微镜和神经病理学。BSCII将协助研究人员进行动物模型和行为测试，使他们能够克服开发和表征新啮齿动物模型的挑战，特别是行为表型，这可能超出了他们的专业领域。该核心将提供目前最完善的NeuroAIDS模型，包括HIV脑病的免疫缺陷小鼠模型，诱导型和组织特异性转基因，敲除或敲除模型，细胞移植模型，病毒载体递送等。将提供学习和记忆以及周围神经病认知变化的行为测试专业知识。在神经模型的育种和表型分析方面的经验，以及可能特别具有挑战性的行为测试，将是一个宝贵的资源。还将协助对人体尸检进行组织学和免疫组织化学评价 和来自患有神经系统疾病（包括AIDS、PML、神经变性疾病和CNS瘤形成）的患者的活组织检查临床样品，从实验动物模型和细胞培养物收获的组织。该核心将提供神经病理学评估、研究设计和数据解释，以及显微镜服务，包括组织样本的激光捕获显微切割（LCM）登记和用于下游分子生物学应用的核酸提取，以及用于高分辨率显微镜分析的细胞和组织的双光子（2P）激发显微镜。这些服务将协助神经艾滋病界的研究人员进行组织病理学分析和转化研究。核心小组还将向调查员及其工作人员提供必要的培训，使他们能够熟练地独立履行任何日常核心职能。