Regulation of Armadillo dependent transcription in Drosophila

果蝇中犰狳依赖性转录的调控

基本信息

  • 批准号:
    8495661
  • 负责人:
  • 金额:
    $ 26.32万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2013
  • 资助国家:
    美国
  • 起止时间:
    2013-09-10 至 2018-06-09
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Wnt signaling plays an important role in animal development, and misregulation of the pathway contributes to various human diseases, including a variety of cancers. The proposed experiments are designed to identify and characterize regulators of the homologous Wingless (Wg) signaling pathway in Drosophila. Though many of the core components of the pathway have been identified, factors involved in the cell-specific effects of Wg signaling on gene expression patterns are still poorly understood. The experiments described in this proposal focus on elucidating how Wg signaling alters gene expression in various tissues through the action of the transcriptional coactivator, Armadillo (Arm). To identify regulators of Arm transcriptional activity, we performed a small-scale genetic screen and we identified 15 potential regulators of Arm activity. One gene identified in the screen was mastermind (Mam), a previously characterized coactivator in the Notch signaling pathway. The first two aims of this proposal are focused on testing the hypothesis that Mam-RC, a naturally occurring short isoform of Mam, is a tissue-specific regulator of Arm activity. Experiments outlined in Specific Aim 1 are designed to determine the molecular mechanism by which Mam-RC regulates Arm transcriptional activity. We propose cell culture and biochemical experiments that will test for both physical and functional interactions of Mam-RC with known components of the Arm transcription complex. Specific Aim 2 outlines experiments designed to test the hypothesis that Mam-RC is a tissue-specific regulator of Wg signaling in vivo, by manipulating the levels of mam-RC expression in various tissues in the developing fly. In addition to Mam-RC, we identified other possible regulators of Arm; Specific Aim 3 outlines experiments designed to characterize the role of these other genes in regulating Arm both in vivo and in cell culture. Given the evolutionary conservation of the pathway components in all animals, findings from our studies of these novel regulators of Arm-dependent transcription may provide greater insight into both human development and disease.
描述(申请人提供):WNT信号在动物发育中起着重要的作用,而该通路的错误调节导致了各种人类疾病,包括各种癌症。拟议的实验旨在识别和表征果蝇同源无翼(Wg)信号通路的调节因子。尽管已经确定了该途径的许多核心成分,但参与Wg信号对基因表达模式的细胞特异性影响的因素仍然知之甚少。本提案中描述的实验侧重于阐明Wg信号如何通过转录辅助激活因子Armadillo(ARM)的作用改变各种组织中的基因表达。为了确定ARM转录活动的调节因子,我们进行了一项小规模的基因筛查,我们确定了15个潜在的ARM活性调节因子。在屏幕上发现的一个基因是主脑(Mam),它以前被描述为Notch信号通路中的辅助激活因子。这项提议的前两个目标集中于检验这样的假设,即Mam-RC是一种自然产生的短亚型Mam-RC,是一种组织特异性的ARM活动调节因子。特定目标1中概述的实验旨在确定MAM-RC调节ARM转录活性的分子机制。我们建议进行细胞培养和生化实验,以测试MAM-RC与ARM转录复合体的已知成分之间的物理和功能相互作用。特定目的2概述了旨在通过操纵发育中的苍蝇不同组织中MAM-RC的表达水平来验证Mam-RC是体内Wg信号的组织特异性调节器这一假设的实验。除了MAM-RC,我们还确定了ARM的其他可能的调节因子;特殊目的3概述了旨在表征这些其他基因在体内和细胞培养中调节ARM的作用的实验。鉴于所有动物的途径成分在进化上的保守性,我们对这些依赖于手臂的转录调控因子的研究结果可能会为人类发育和疾病提供更好的洞察力。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Jennifer Kennell其他文献

Jennifer Kennell的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Jennifer Kennell', 18)}}的其他基金

CK1delta/epsilon Inhibits Apoptosis in Drosophila
CK1delta/epsilon 抑制果蝇细胞凋亡
  • 批准号:
    7228415
  • 财政年份:
    2005
  • 资助金额:
    $ 26.32万
  • 项目类别:
CK1delta/epsilon Inhibits Apoptosis in Drosophila
CK1delta/epsilon 抑制果蝇细胞凋亡
  • 批准号:
    6937601
  • 财政年份:
    2005
  • 资助金额:
    $ 26.32万
  • 项目类别:
CK1delta/epsilon Inhibits Apoptosis in Drosophila
CK1delta/epsilon 抑制果蝇细胞凋亡
  • 批准号:
    7050184
  • 财政年份:
    2005
  • 资助金额:
    $ 26.32万
  • 项目类别:

相似海外基金

CAREER: Biochemical and Structural Mechanisms Controlling tRNA-Modifying Metalloenzymes
职业:控制 tRNA 修饰金属酶的生化和结构机制
  • 批准号:
    2339759
  • 财政年份:
    2024
  • 资助金额:
    $ 26.32万
  • 项目类别:
    Continuing Grant
Systematic manipulation of tau protein aggregation: bridging biochemical and pathological properties
tau 蛋白聚集的系统操作:桥接生化和病理特性
  • 批准号:
    479334
  • 财政年份:
    2023
  • 资助金额:
    $ 26.32万
  • 项目类别:
    Operating Grants
Diurnal environmental adaptation via circadian transcriptional control based on a biochemical oscillator
基于生化振荡器的昼夜节律转录控制的昼夜环境适应
  • 批准号:
    23H02481
  • 财政年份:
    2023
  • 资助金额:
    $ 26.32万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Leveraging releasable aryl diazonium ions to probe biochemical systems
利用可释放的芳基重氮离子探测生化系统
  • 批准号:
    2320160
  • 财政年份:
    2023
  • 资助金额:
    $ 26.32万
  • 项目类别:
    Standard Grant
Biochemical Mechanisms for Sustained Humoral Immunity
持续体液免疫的生化机制
  • 批准号:
    10637251
  • 财政年份:
    2023
  • 资助金额:
    $ 26.32万
  • 项目类别:
Structural and biochemical investigations into the mechanism and evolution of soluble guanylate cyclase regulation
可溶性鸟苷酸环化酶调节机制和进化的结构和生化研究
  • 批准号:
    10604822
  • 财政年份:
    2023
  • 资助金额:
    $ 26.32万
  • 项目类别:
Enhanced Biochemical Monitoring for Aortic Aneurysm Disease
加强主动脉瘤疾病的生化监测
  • 批准号:
    10716621
  • 财政年份:
    2023
  • 资助金额:
    $ 26.32万
  • 项目类别:
Converting cytoskeletal forces into biochemical signals
将细胞骨架力转化为生化信号
  • 批准号:
    10655891
  • 财政年份:
    2023
  • 资助金额:
    $ 26.32万
  • 项目类别:
Chemical strategies to investigate biochemical crosstalk in human chromatin
研究人类染色质生化串扰的化学策略
  • 批准号:
    10621634
  • 财政年份:
    2023
  • 资助金额:
    $ 26.32万
  • 项目类别:
Examination of risk assessment and biochemical assessment of fracture development focusing on the body composition of patients with rheumatoid arthritis
关注类风湿性关节炎患者身体成分的骨折发生风险评估和生化评估检查
  • 批准号:
    22KJ2600
  • 财政年份:
    2023
  • 资助金额:
    $ 26.32万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了