Skyscan 1172 Ex-vivo MicroComputed Tomography System

描述（由申请人提供）：这是一份为购买Sky scan 1172离体微型计算机断层扫描（micro CT）系统申请资金的修订提案，用于支持纽约大学牙科学院（NYUCD）和纽约大学医学院（NYUSoM）邻近学院的多学科资助研究。在过去两年中，纽约大学从事骨骼和生物材料研究的研究人员数量大大增加。临床x射线计算机断层扫描（CT）技术已经适应于用微CT成像更小的物体和更大的分辨率，这已经成为研究矿化组织微结构的最新技术。这项技术使得研究骨质疏松和代谢性骨病、癌症转移、骨异常表型、异位矿化和植入生物材料的小动物模型的三维形态细节成为可能。来自纽约大学、纽约大学医学院和纽约大学关节疾病医院（HJD）的13个资助院系被确定为离体微型CT的主要初始用户。该仪器将成为位于NYUSoM和HJD之间的牙科学院的空间共享资源。学校将支持服务合同成本，并100%为设施经理提供一名技术人员。Sky scan 1172系统是该公司的第二代仪器，由配备5¿m点尺寸x射线源和12位CCD相机的台式锥束扫描仪组成，使样品细节可探测性<1¿m；一台双核控制器计算机和戴尔双核六核处理器工作站在集群配置中有效运行3D重建软件。图像处理软件可用于任何基于windows的校园计算机，用于标准的2D和3D硬组织形态测量分析和动画。通过微型CT的非破坏性成像和精确定量，以及冷却阶段和微定位阶段的存在，使标本可用于后续的分子或免疫组织化学分析，提供比其他方式更完整的表征，有助于减少实验中的动物数量。获得该设备将使我们的机构能够大大提高与人类疾病的病理生理过程和动物模型直接相关的生物医学研究的效率。

DESCRIPTION (provided by applicant): This is a revised proposal to request funds for the purchase of a Sky scan 1172 ex-vivo micro Computed Tomography (micro CT) system to support multi-disciplinary funded research at the adjacent schools of the New York University College of Dentistry (NYUCD) and the New York University School of Medicine (NYUSoM). The number of investigators engaged in bone and biomaterials research at NYU has greatly increased in the last two years. Techniques for clinical x- ray computed tomography (CT) have been adapted for imaging of smaller objects with larger resolution by micro CT, which has now become the state of the art technology to study mineralized tissue microarchitecture. This technology has made it possible to study the 3D morphological detail of small animal models of osteoporosis and metabolic bone disease, cancer metastasis, bone abnormality phenotypes, ectopic mineralization, and implanted biomaterials. A group of 13 funded faculties from the NYUCD, NYUSoM and the NYU-Hospital for Joint Diseases (HJD) are identified as major initial users of the ex-vivo micro CT. The instrument will be a shared resource in space in the College of Dentistry which is situated between NYUSoM and HJD. The Schools will support service contract costs and 100% of a technician for the facility manager. The Sky scan 1172 system, the second generation instrument from this company, consists of a desktop Cone-Beam scanner equipped with a 5¿m spot size x-ray source and 12-bit CCD camera, resulting in specimen detail detectability <1¿m; a dual core controller computer and Dell dual six-core processor workstations in a cluster configuration to efficiently run the 3D reconstruction software. The image processing software may be used on any Windows-based campus computer for standard 2D and 3D hard tissue morphometric analysis and animation. The non-destructive imaging and accurate quantification by micro CT and existence of a cooling stage together with a micro-positioning stage allows the specimen to be used for subsequent molecular or immunohistochemical analysis, providing a more complete characterization than otherwise possible contributing to minimization of animal numbers in experiments. Acquisition of this equipment will enable our institutions to greatly improve the efficiency of biomedical research directly related to pathophysiological processes and animal models of human diseases.