Nikon A1Rsi resonant spectral confocal microscope

描述（由申请人提供）：该提案要求提供资金，用于购买Nikon A1 Rsi共振光谱共聚焦显微镜，该显微镜具有高空间和时间分辨率，焦点保持和环境控制，用于对活体和固定生物样品进行成像。这种先进的最先进的共聚焦显微镜将是一个共享资源，位于斯坦福大学一个完善的，多用户，光学和电子显微镜设施：细胞科学成像设施（CSIF）。该设施可供斯坦福大学的整个研究社区使用。所要求的共聚焦显微镜将支持9个主要用户的NIH资助项目。这些项目调查了NIH支持的应用于活的和固定的生物样品的广泛研究，包括：（1）干细胞更新和分化的调节（果蝇，富勒）;（2）病毒复制、装配和从感染细胞中排出的分子机制（小鼠，人，阿尔文）;（3）视觉通路中神经发育的分析（小鼠、大鼠、Shatz）;（4）转基因小鼠的肺动脉高压（小鼠，Rabinovich）;（5）原肠胚形成中上皮细胞重排的分子机制（fish，纳尔逊）;（6）工程化3D体外小生境以揭示细胞生物力学的基本原理（人，Heilshorn）;（7）纤毛病中的初级纤毛生物发生和膜运输（人，Nachury）;（8）癫痫发展背后的神经元机制的表征和建模（小鼠，大鼠，Prince）;（9）干细胞分裂和命运的动态分析（人，Blau）。这些研究调查了各种模式生物和人体组织中的关键功能和结构问题。这项生物医学研究影响人类健康和疾病的各个方面，从干细胞发育到理解感觉生物学的细胞和分子基础。所有这些都需要最先进的共焦仪器来对固定和活体样品进行成像，这是由所要求的Nikon A1 Rsi共振光谱激光扫描共焦显微镜最有效地提供的，该显微镜配备了用于信号分离的光谱检测器、用于快速成像的共振扫描仪和压电z驱动器、用于样品无漂移延时成像的焦点保持环境控制。

DESCRIPTION (provided by applicant): This proposal requests funds to purchase a Nikon A1Rsi resonant spectral confocal microscope with high spatial and temporal resolution, focus-maintenance and environmental control for imaging both live and fixed biological samples. This advanced state-of-the-art confocal microscope will be a shared resource, located in a well-established, multi-user, light and electron microscopy facility at Stanford University: the Cell Sciences Imaging Facility (CSIF). This facility is accessible to Stanford University's entire research community. The requested confocal microscope will support NIH funded projects from 9 major users. These projects investigate a wide range of NIH supported research applied to both live and fixed biological samples, including: (1) Regulation of stem cell renewal and differentiation (Drosophila, Fuller); (2) Molecular mechanisms of viral replication, assembly and egress from infected cells (mice, human, Arvin); (3) Analysis of neural development in the visual pathway (mice, rat, Shatz); (4) Pulmonary hypertension in genetically modified mice (mice, Rabinovich); (5) Molecular mechanisms underlying epithelial cell rearrangements in gastrulation (fish, Nelson); (6) Engineering 3D in-vitro niches to reveal fundamentals of cellular biomechanics (human, Heilshorn); (7) Primary cilium biogenesis and membrane trafficking in ciliopathies (human, Nachury); (8) Characterization and modeling of the neuronal mechanisms underlying the development of epilepsy (mice, rat, Prince); (9) Dynamic analyses of stem cell division and fate (human, Blau). These studies investigate critical functional and structural questions in a variety of model organisms and human tissues. This biomedical research impacts diverse aspects of human health and disease, ranging from stem cell development to understanding the cellular and molecular basis of sensory biology. All require state-of-the-art confocal instrumentation for imaging both fixed and live samples which is most effectively provided by the requested Nikon A1Rsi resonant spectral laser-scanning confocal microscope equipped with spectral detector for signal unmixing, resonant scanner and piezo z-drive for fast imaging, environmental control with focus maintenance for drift free time lapse imaging of samples.