Nikon A1Rsi resonant spectral confocal microscope

描述（由申请人提供）：本申请申请资金用于购买一台尼康A1Rsi共振光谱共聚焦显微镜，该显微镜具有高时空分辨率、聚焦维持能力和环境控制能力，可用于活体和固定生物样品的成像。这台先进的共聚焦显微镜将成为共享资源，位于斯坦福大学一个完善的多用户光学和电子显微镜设施：细胞科学成像设施（CSIF）。斯坦福大学的整个研究界都可以使用这个设施。请求的共聚焦显微镜将支持NIH资助的9个主要用户的项目。这些项目调查了广泛的NIH支持的应用于活体和固定生物样本的研究，包括：(1)干细胞更新和分化的调控（果蝇，富勒）；(2)病毒复制、组装和从感染细胞（小鼠、人、Arvin）中输出的分子机制；(3)视觉通路神经发育分析（小鼠、大鼠、Shatz）；(4)转基因小鼠肺动脉高压（小鼠，Rabinovich）；(5)原肠形成过程中上皮细胞重排的分子机制（fish, Nelson）；(6)工程3D体外小生境揭示细胞生物力学基础（人类，Heilshorn）；(7)原发性纤毛生物发生和纤毛病的膜贩运（人类，Nachury）；(8)癫痫发生的神经元机制表征与建模（小鼠、大鼠、Prince）；(9)干细胞分裂与命运的动态分析（human, Blau）。这些研究调查了各种模式生物和人体组织的关键功能和结构问题。这种生物医学研究影响着人类健康和疾病的各个方面，从干细胞发育到理解感觉生物学的细胞和分子基础。所有这些都需要最先进的共聚焦仪器来成像固定和活体样品，其中最有效的是尼康A1Rsi谐振光谱激光扫描共聚焦显微镜，该显微镜配备了用于信号解混的光谱探测器，用于快速成像的谐振扫描仪和压电z驱动器，以及用于样品无漂移时移成像的环境控制和焦点维护。

DESCRIPTION (provided by applicant): This proposal requests funds to purchase a Nikon A1Rsi resonant spectral confocal microscope with high spatial and temporal resolution, focus-maintenance and environmental control for imaging both live and fixed biological samples. This advanced state-of-the-art confocal microscope will be a shared resource, located in a well-established, multi-user, light and electron microscopy facility at Stanford University: the Cell Sciences Imaging Facility (CSIF). This facility is accessible to Stanford University's entire research community. The requested confocal microscope will support NIH funded projects from 9 major users. These projects investigate a wide range of NIH supported research applied to both live and fixed biological samples, including: (1) Regulation of stem cell renewal and differentiation (Drosophila, Fuller); (2) Molecular mechanisms of viral replication, assembly and egress from infected cells (mice, human, Arvin); (3) Analysis of neural development in the visual pathway (mice, rat, Shatz); (4) Pulmonary hypertension in genetically modified mice (mice, Rabinovich); (5) Molecular mechanisms underlying epithelial cell rearrangements in gastrulation (fish, Nelson); (6) Engineering 3D in-vitro niches to reveal fundamentals of cellular biomechanics (human, Heilshorn); (7) Primary cilium biogenesis and membrane trafficking in ciliopathies (human, Nachury); (8) Characterization and modeling of the neuronal mechanisms underlying the development of epilepsy (mice, rat, Prince); (9) Dynamic analyses of stem cell division and fate (human, Blau). These studies investigate critical functional and structural questions in a variety of model organisms and human tissues. This biomedical research impacts diverse aspects of human health and disease, ranging from stem cell development to understanding the cellular and molecular basis of sensory biology. All require state-of-the-art confocal instrumentation for imaging both fixed and live samples which is most effectively provided by the requested Nikon A1Rsi resonant spectral laser-scanning confocal microscope equipped with spectral detector for signal unmixing, resonant scanner and piezo z-drive for fast imaging, environmental control with focus maintenance for drift free time lapse imaging of samples.