New Console and Cold Probe for the Duke 600 MHz NMR Spectrometer System

描述（由申请人提供）：杜克大学建议用新的控制台、冷冻系统和1H{13C/15N} 5mm 13C增强冷探头替换、更新和增强其600 MHz核磁共振光谱系统的故障组件。600兆赫光谱仪安置在杜克核磁共振光谱中心，并由其管理，该中心作为所有大学研究项目的核磁共振资源，为研究三角和东南地区的大学和政府实验室的科学家提供核磁共振资源。它支持、促进和培训科学家使用核磁共振进行基础生化和生物医学科学的前沿研究。所要求的新设备组件需要替换一个12年的控制台和8年的冷冻系统和探针，现在以不可接受的速度失效。这些组件故障严重影响了许多研究人员的研究活动，这些研究人员依赖杜克大学600兆赫仪器来开展他们的资助项目。该工具完全订阅了超过24/7时间表的请求。然而，最近，由于控制台和我们的第一代冷冻系统和冷探针中关键组件失效相关的意外维护和维修，可用时间有限。瓦里安Inova控制台不再生产，已经过时了两代，并已被新一代安捷伦DirectDrive2光谱仪所取代，这一事实使仪器可用于研究的时间受到限制。我们系统的新部件不再制造和翻新，使用过的部件通常也不能立即可用。我们的600 MHz核磁共振系统所需的新设备组件提供了一种具有成本效益的策略，以恢复和增强这台骨干光谱仪，成本约为新系统的一半，因为我们的14.09T牛津磁铁状态良好，具有卓越的均匀性和可忽略的漂移。目前在以下领域的研究活动将通过升级到杜克600兆赫核磁共振光谱仪得到加强：蛋白质组装的研究；蛋白质构象动力学与折叠；膜相关脂质及其合成过程中的酶；酶的结构表征与再设计；蛋白质的细胞内核磁共振光谱；与HIV疫苗设计相关的免疫原性肽和磷脂的抗体识别；细胞信号;多维核磁共振数据快速获取方法开发。

DESCRIPTION (provided by applicant): Duke University proposes to replace, update, and enhance failing components of its 600 MHz NMR spectroscopy system with a new console, cryo-system, and 1H{13C/15N} 5mm 13C-enhanced cold probe. The 600 MHz spectrometer is housed in and is managed by the Duke NMR Spectroscopy Center that serves as an NMR resource for all University research projects and for scientists at universities and government laboratories in the Research Triangle and the Southeastern region. It supports, facilitates and trains scientists in the use of NMR for frontier research in basic biochemical and biomedical sciences. The requested new equipment components are needed to replace a 12-year-old console and 8-year-old cryo- system and probe that are now failing at an unacceptable rate. These component failures have significantly impacted research activities of numerous investigators who depend on the Duke 600 MHz instrument to pursue their funded projects. The instrument is fully subscribed with requests that exceed a 24/7 schedule. Recently, however, the available time has been limited due to unanticipated maintenance and repairs associated with failure of critical components in the console and our first generation cryo-system and cold probe as they have aged. This limitation in the instrument time available for research is compounded by the fact that the Varian Inova console is no longer manufactured, is two generations out of date, and has been replaced by a new generation Agilent DirectDrive2 spectrometer. New parts for our system are no longer manufactured and refurbished, used parts, are often not immediately available either. The new equipment components requested for our 600 MHz NMR system provides a cost effective strategy to restore and enhance this workhorse spectrometer at a cost of approximately half that of a new system since our 14.09T Oxford magnet is in excellent condition and performs with superior homogeneity and negligible drift. Current research activities in the following areas will be enhanced by this upgrade to the Duke 600 MHz NMR spectrometer: studies of protein assemblies; protein conformational dynamics and folding; membrane associated lipids and enzymes involved in their synthesis; enzyme structural characterization and redesign; in-cell NMR spectroscopy of proteins; antibody recognition of immunogenic peptides and phospholipids associated with HIV vaccine design; cellular signaling; and methods development for rapid acquisition of multidimensional NMR data.