Multiphoton Imaging and Electrophysiology Workstation

描述(由申请人提供)：我们请求资金购买共享的多光子成像和电生理工作站。该工作站将包括一个专用的多光子固定平台直立显微镜(奥林巴斯FluoView 1000 MPE)，配备适合于对活体脑片或视网膜移植进行成像的光学元件，以及电子显微操纵器和其他辅助设备(电极拉出器、振动刀、灌注室等)。多光子成像与膜片钳电生理学相结合的实验所必需的。用户群由艺术与科学学院和范德比尔特大学医学中心的细胞和分子神经学家组成，他们使用先进的成像和电生理技术研究神经科学中的广泛问题，并且都有神经组织的多光子成像的关键实验需求，包括穿透深度的增加、空间受限的成像和刺激、光毒性的降低以及深红外多光子成像所提供的可见光刺激的缺乏。所需的奥林巴斯FluoView 1000 MPE系统根据用户群体的需求进行了独特的配置，特别关注用于电生理实验的特定需求的最高性能浸入式物镜，以及用于向活体视网膜传递光和图案刺激的双传输光路 准备工作。该仪器将放置在生物科学大楼/医学研究大楼III(BSB/MRBIII)7楼的Vanderbilt细胞成像共享资源(CISR)中并由其维护。这座大楼容纳了许多用户，包括中科院的用户，所有用户都可以方便地进入。这台MPE显微镜的专业知识和机构支持都是出类拔萃的。活塞博士和威尔斯博士分别是国际科学研究中心的科学总监和常务董事，他们都是世界知名的多光子成像专家。CSR拥有18年的历史，为275名拥有尖端成像专业知识的调查人员提供协助。范德比尔特大学文理学院和医学中心已承诺提供30万美元的资金，以加强该仪器的购买和运营。

DESCRIPTION (provided by applicant): We request funds for the purchase of a shared Multiphoton Imaging and Electrophysiology Workstation. This workstation will consist of a dedicated multiphoton fixed stage upright microscope (Olympus Fluoview 1000 MPE) equipped with optics appropriate for imaging living brain slices or retinal explants, as well as electronics micromanipulators and other support items (electrode puller, vibratome, perfusion chamber, etc.) necessary for experiments combining multiphoton imaging with patch-clamp electrophysiology. The user group is made up of cellular and molecular neuroscientists of both the College of Arts and Sciences and the Medical Center of Vanderbilt University who study a broad range of problems in neuroscience using advanced imaging and electrophysiological techniques, and all have a critical experimental needs for multiphoton imaging of neural tissue in terms of the increased depth of penetration, the spatially restricted imaging and stimulation, the decrease in phototoxicity and the absence of visible light stimulation afforded by deep IR multiphoton imaging. The Olympus Fluoview 1000 MPE system requested has been uniquely configured based on the needs of the user group with significant attention to the highest performing immersion objective lenses for the specific needs of electrophysiology experiments as well as a dual transmitted light path for delivering light and pattern stimuli to living retinal preparations. The instrument will be located in and maintained by the Vanderbilt Cell Imaging Shared Resource (CISR) on the 7th floor of the Biological Sciences Building/Medical Research Building III (BSB/MRBIII). This building houses many of the users, including those from CAS, and is conveniently accessible to all users. The expertise and institutional support for this MPE microscope are exceptional. Drs. Piston and Wells, Scientific Director and Managing Director of the CISR, respectively, are world-renowned experts in multiphoton imaging. The CISR has an 18-year history of assisting over 275 investigators with cutting-edge imaging expertise. Vanderbilt University College of Arts and Sciences and Medical Center have committed $300,000 in funds to enhance the purchase and operation of the instrument.