Multiphoton Imaging and Electrophysiology Workstation

描述（由申请人提供）：我们申请资金购买共享的多光子成像和电生理工作站。该工作站将包括专用的多光子固定级直立显微镜（Olympus Fluoview 1000 MPE），配备适合成像活体脑切片或视网膜外植体的光学器件，以及电子微操作器和其他支持项目（电极拉杆、振动器、灌注室等），用于多光子成像与膜片钳电生理学相结合的实验。用户组由范德比尔特大学文理科学院和医学中心的细胞和分子神经科学家组成，他们使用先进的成像和电生理技术研究神经科学领域的广泛问题，并且都对神经组织的多光子成像有关键的实验需求，包括增加穿透深度，空间限制成像和刺激，光毒性的降低和深红外多光子成像所提供的可见光刺激的缺乏。所要求的奥林巴斯Fluoview 1000 MPE系统已根据用户组的需求进行了独特的配置，特别关注最高性能的浸入式物镜，以满足电生理学实验的特定需求，以及双透射光路，用于向活视网膜提供光和图案刺激

DESCRIPTION (provided by applicant): We request funds for the purchase of a shared Multiphoton Imaging and Electrophysiology Workstation. This workstation will consist of a dedicated multiphoton fixed stage upright microscope (Olympus Fluoview 1000 MPE) equipped with optics appropriate for imaging living brain slices or retinal explants, as well as electronics micromanipulators and other support items (electrode puller, vibratome, perfusion chamber, etc.) necessary for experiments combining multiphoton imaging with patch-clamp electrophysiology. The user group is made up of cellular and molecular neuroscientists of both the College of Arts and Sciences and the Medical Center of Vanderbilt University who study a broad range of problems in neuroscience using advanced imaging and electrophysiological techniques, and all have a critical experimental needs for multiphoton imaging of neural tissue in terms of the increased depth of penetration, the spatially restricted imaging and stimulation, the decrease in phototoxicity and the absence of visible light stimulation afforded by deep IR multiphoton imaging. The Olympus Fluoview 1000 MPE system requested has been uniquely configured based on the needs of the user group with significant attention to the highest performing immersion objective lenses for the specific needs of electrophysiology experiments as well as a dual transmitted light path for delivering light and pattern stimuli to living retinal preparations. The instrument will be located in and maintained by the Vanderbilt Cell Imaging Shared Resource (CISR) on the 7th floor of the Biological Sciences Building/Medical Research Building III (BSB/MRBIII). This building houses many of the users, including those from CAS, and is conveniently accessible to all users. The expertise and institutional support for this MPE microscope are exceptional. Drs. Piston and Wells, Scientific Director and Managing Director of the CISR, respectively, are world-renowned experts in multiphoton imaging. The CISR has an 18-year history of assisting over 275 investigators with cutting-edge imaging expertise. Vanderbilt University College of Arts and Sciences and Medical Center have committed $300,000 in funds to enhance the purchase and operation of the instrument.