In Vivo Ultrasound Imaging System (VEVO 2100, VisualSonics)

描述（由申请人提供）：本提案的目标是为小动物模型的体内超声成像系统（VEVO 2100, Visual sonic, Toronto, Canada）获得资金。这是一种高分辨率的体内超声微成像系统，专为小动物模型的综合解剖和生理的非侵入性实时研究而设计。该系统的空间分辨率从100米到30米，深度分别从36毫米到12毫米，这是目前可用的最高分辨率。此外，实时成像系统允许超声引导注射，将生物试剂（微颗粒、基因、siRNA、microRNA和干细胞）递送到小动物模型的不同器官。加州大学戴维斯分校目前还没有这项技术。该系统将为美国国立卫生研究院资助的一大批研究人员提供服务。我们研究团队的一个独特之处在于其教师的多样性。我们的团队成员处于职业生涯的不同阶段，来自五个不同学院/学院（医学院、兽医学院、工程学院、农业与环境科学学院和生物科学学院）的多个部门。这组研究人员参与了几个多学科合作项目，包括心血管、血管、

DESCRIPTION (provided by applicant): The goal of this proposal is to secure funding for an in vivo ultrasound imaging system for small animal models (VEVO 2100, Visual Sonics, Toronto, Canada). This is a high-resolution in vivo ultrasound micro- imaging system designed specifically for non-invasive real-time studies of integrated anatomy and physiology in small animal models. The system offers spatial resolutions from 100 ¿m to 30 ¿m with depths from 36 mm down to 12 mm respectively, which represents the highest resolution available today. Moreover, the real-time imaging system allows for ultrasound-guided injections for delivery of biological reagents (micro particles, genes, siRNA, microRNA, and stem cells) to different organs in small animal models. This technology is not currently available at UC Davis. The proposed system will serve a large group of NIH-funded investigators. A unique feature of our research team is the diversity of its faculty. Our team members are at various stages of their careers and are members of multiple departments from five different schools/colleges (School of Medicine, School of Veterinary Medicine, School of Engineering, College of Agricultural and Environmental Sciences, and College of Biological Sciences). This group of investigators is involved in several multidisciplinary collaborative projects that include cardiovascular, vascular, pulmonary, renal, and central nervous systems research. The uses of small animal models, including genetically targeted models, have paved the way for major advances in Medicine during recent years. Indeed, these small animal models are critical for the preclinical studies of diverse classes of diseases including cardiovascular and vascular diseases, pulmonary diseases, cancers, and neurological diseases. The ability to directly quantify the anatomical and physiologic information at a high resolution from embryos to adulthood as well as the use of ultrasound-guided delivery of biological reagents will greatly advance our knowledge and translation of these models to the treatment of human diseases. This proposal will enable the application of non-invasive in vivo high-resolution assessment of disease models that will foster further collaborative translational research projects.