XF96 Extracellular Flux Analyzer

描述：本申请的目的是为Seahorse XF96（细胞外通量，96孔）分析仪获得资金。最近，对细胞代谢和能量利用的兴趣的复苏几乎影响了细胞生物学的所有研究领域。这包括对代谢、神经退行性和心血管疾病、癌症、药物安全、基本细胞生理学、免疫学和炎症过程的研究。这一分析在不同学科之间的重要性反映在为S10设备拨款申请而联合起来的研究人员的研究兴趣范围上。这种重新燃起的兴趣在很大程度上是由各种“组学”分析驱动的，这些分析经常识别动物模型和患者样本中细胞之间能量使用的变化。海马生物科学已经满足了这种新的兴趣，并产生了一系列的机器，大大提高了吞吐量和易用性。他们的仪器对于核心设施来说是完美的，因为用户可以快速训练来分析和询问他们感兴趣的实验样本。XF96能够同时实时测量原代细胞或分离线粒体的糖酵解和氧化磷酸化。96孔微孔板格式允许更小的细胞样品尺寸，在贴壁和悬浮细胞上实时测量多种分析物（目前是氧气和质子）。此外，可以自动将多达四种测试化合物（药物或底物或激动剂或拮抗剂）添加到每个孔中，并在添加每种试剂之前和之后进行测量。最后，这项技术填补了目前大多数研究实验室缺乏的关键利基，开辟了细胞生理学和疾病状态激活的新见解和范式。

DESCRIPTION: The goal of this application is to obtain funding for a Seahorse XF96 (extracellular flux, 96 well) analyzer. A resurgence of interest in cell metabolism and energy use has recently impacted almost all areas of research on cell biology. This includes research into metabolic, neurodegenerative and cardiovascular diseases, cancer, drug safety, basic cell physiology, immunology and the processes of inflammation. The importance of this analysis among various disciplines is mirrored by the range in research interests of the investigators joining together for this S10 equipment grant application. Much of this renewed interest has been driven by the various "omic" analyses, which often identify variations in energy use among cells in both animal models and patient samples. Seahorse Bioscience has met this renewed interest and generated a series of machines that greatly improve the throughput and ease of use. Their instruments are perfect for a core facility as users can be rapidly trained to analyze and interrogate their experimental samples of interest. The XF96 is capable of measuring both glycolysis and oxidative phosphorylation simultaneously on primary cells or isolated mitochondria continuously in real time. The 96-well microplate format allows for smaller cell sample sizes to measure multiple analytes (currently oxygen and protons) in real time on adherent and suspension cells in situ. In addition, up to four test compounds (drugs or substrates or agonists or antagonists) may be added automatically to each well with measurements performed before and after addition of each reagent. Lastly, this technology fills a critical niche currently absent from most research labs by opening up new insights and paradigms of activation in cell physiology and disease states.