Leica LMD 7000 Laser Capture Microdissection Microscope

Leica LMD 7000 激光捕获显微切割显微镜

• 批准号: 8447882
• 负责人: Siegfried Janz
• 金额: $ 21.69万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-11 至 2014-07-10
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447882
• 关键词: Applications Grants; Award; Biomedical Research; Brain; Candida albicans; Carrier Proteins; Cells; Chromosomes; Cystic Fibrosis; Drug Delivery Systems; Family suidae; Funding; Genetic; Genetic Recombination; Head and Neck Cancer; Human; Human Resources; Immunocompromised Host; Immunologics; Individual; Iowa; Lasers; Letters; Lymphoma; Malignant Neoplasms; Malignant neoplasm of brain; Malignant neoplasm of pancreas; Medicine; Microdissection; Microscope; Microscopy; Modeling; Molecular Genetics; Opportunistic Infections; Organ; Pathway interactions; Patients; Plasma Cell Neoplasm; Prevention approach; Prognostic Marker; Research; Research Personnel; Research Project Grants; Structure of mucous membrane of nose; System; Therapeutic antibodies; Tissue Sample; Training; United States National Institutes of Health; Universities; Virulence Factors; Yeasts; anticancer research; cleft lip and palate; college; experience; follow-up; human disease; improved; instrument; instrumentation; laser capture microdissection; neoplastic cell; novel; public health relevance; repaired; research facility; tool

DESCRIPTION (provided by applicant): This instrumentation grant proposal requests funds for a Leica LMD 7000 laser microdissection microscope/system, a critical tool for ongoing NIH-supported biomedical research projects at the University of Iowa (UI). The proposed microscope will provide high-end capabilities for seeking out and collecting very specific, targeted cell and tissue samples for follow-up studies on the molecular genetics and immunologic underpinnings of human diseases of great relevance for public health. The UI currently has one laser microdissection microscope to serve the entire campus; however, the instrument is obsolete, difficult to use, expensive to repair, and has recently experienced tons of downtime. Clearly, it needs to be replaced with a modern instrument that will be adequate for the research of the 11 UI investigators that take part in this application Five of 11 investigators will use the LMD 7000 for cancer research: Siegfried Janz seeks to understand the genetic pathways of plasma cell tumor formation; Andean Burnett attempts to develop targeted therapies for patients with head and neck cancer; Hasem Habelhah elucidates mechanisms of cFLIP expression and RIP1 cleavage in tumor cells; George Weiner evaluates therapeutic antibodies for use in novel lymphoma treatment and prevention approaches; and Dawn Quelle assesses the potential utility of PARF as a prognostic biomarker for pancreatic and brain cancer. 6 of 11 investigators will take advantage of LMD 7000-assisted laser microdissection for research unrelated to cancer: Josef Comeron determines genetic recombination rates along individual chromosomes; Jeffery Murray and Martine Dunnwald perform genetic studies on cleft lip and palate formation; David Stolz and Aliye Uc examine organ damage in a newly developed pig model of human cystic fibrosis; Maureen Donovan studies transporter proteins in the nasal mucosa for improved drug delivery systems to the brain; and David Soll evaluates factors of pathogenicity in the yeast, Candida albicans, the frequent underlying reason of opportunistic infections in immunosuppressed patients. All studies described above - currently supported by $16,853,566 of active NIH research funding - would greatly benefit from the acquisition of a Leica LMD 7000. If this application will be awarded, the system will be set up in the UI Central Microscopy Research Facility. Dedicated facility personnel will administer and maintain the instrument, provide high-level technical assistance and training, and make it available "24/7" in order to accommodate as many users as possible. This application enjoys strong institutional commitment, as demonstrated by attached letters of support from the UI VP for Research, Dr. Cohen, and the Dean of the College of Medicine, Dr. Rothman.

描述（由申请人提供）：该仪器拨款提案要求为Leica LMD 7000激光显微切割显微镜/系统提供资金，这是爱荷华州大学（UI）正在进行的NIH支持的生物医学研究项目的关键工具。拟议的显微镜将提供高端功能，用于寻找和收集非常具体的靶向细胞和组织样本，用于对与公共卫生具有重大意义的人类疾病的分子遗传学和免疫学基础进行后续研究。UI目前有一台激光显微切割显微镜为整个校园服务;然而，该仪器已经过时，难以使用，维修费用昂贵，最近经历了大量的停机时间。显然，它需要更换为一种现代仪器，该仪器将足以用于参与该应用的11名UI研究人员的研究。11名研究人员中有5名将使用LMD 7000 癌症研究：齐格弗里德扬兹试图了解浆细胞肿瘤形成的遗传途径;安第斯伯内特试图为头颈癌患者开发靶向治疗;哈泽姆哈贝尔哈阐明cFLIP表达和RIP 1切割在肿瘤细胞中的机制;乔治韦纳评估用于新型淋巴瘤治疗和预防方法的治疗性抗体; Dawn Quelle评估了PARP作为胰腺癌和脑癌预后生物标志物的潜在效用。 11名研究人员中的6名将利用LMD 7000辅助激光显微切割进行与癌症无关的研究：Josef Comeron确定了沿着个体染色体的遗传重组率; Jeffery Murray和Martine Dunnwald对唇腭裂形成进行了遗传研究;大卫Stolz和Aliye Uc在新开发的人类囊性纤维化猪模型中检查了器官损伤;莫琳·多诺万（Maureen Donovan）研究鼻粘膜中的转运蛋白，以改善药物向大脑的输送系统;大卫·索尔（David Soll）评估了酵母菌（白色念珠菌）的致病因素，白色念珠菌是免疫抑制患者机会性感染的常见潜在原因。 上述所有研究--目前由美国国立卫生研究院的16，853，566美元研究基金支持--将大大受益于徕卡LMD 7000的收购。如果这个应用程序将被授予，该系统将被设置在UI中央显微镜研究设施。专门的设施人员将管理和维护仪器，提供高水平的技术援助和培训，并提供“24/7”服务，以满足尽可能多的用户。该应用程序享有强大的机构承诺，如所附的支持信，从UI研究副总裁科恩博士和医学院院长罗斯曼博士证明。