Seahorse XF96 Extracellular Flux Analyzer

描述：我们正在申请资金购买SeaHorse XF96细胞外通量分析仪，这是一种以中/高通量方式非侵入性地、在几分钟内分析细胞代谢活动和生物能量状态的仪器。SeaHorse XF96胞外通量分析仪使用简单的微板格式，实时同时测量氧气消耗(细胞呼吸)和胞外酸化速率。结合使用不同的能量底物和可通过四个集成进样口自动输送的特定酶和代谢途径的各种抑制剂，海马胞外通量分析仪可对细胞的氧化磷酸化、糖酵解、营养利用、脂肪酸氧化、耦合呼吸与非耦合呼吸以及剩余呼吸能力进行前所未有的、全面和快速的评估。目前还没有其他单一的仪器能够以96孔的形式进行相同范围的代谢分析。线粒体的功能和适当的底物利用是大多数对人类健康至关重要的生理过程的核心，包括生长、能量动态平衡、体力活动能力和衰老。线粒体功能障碍与多种疾病的发生、发展和/或发病有关，包括代谢性疾病(如胰岛素抵抗和2型糖尿病)、癌症、神经退行性疾病和心血管疾病。所需的海马XF96胞外通量分析仪将使至少12名研究人员(目前无法使用类似的仪器或技术)深入了解用于模拟生理和病理过程的各种细胞和生物(包括原代肝细胞、脂肪细胞、神经元、心肌细胞、癌细胞、肌管和蠕虫)的细胞代谢。海马XF96细胞外通量分析仪非常适合用户的特定需求，其关键因素是该仪器能够：1)监测附着细胞(12名研究人员中有7人使用附着细胞)；2)从少量细胞获得可靠的测量结果(6名用户至关重要)；3)提供中高通量格式，从而能够进行药物筛选(5个项目)和评估对各种生理信号、环境侮辱和遗传改变的反应(对所有项目都很重要)。SeaHorse XF96细胞外通量分析仪在功能组合方面是无与伦比的，这使得它非常适合当前用户的研究。该仪器将放置在多用户适当的环境中，所有用户都可以随时使用，新用户也可以使用。总之，通过对细胞代谢和线粒体功能的全面评估，海马XF96细胞外通量分析仪将促进和加强我们对代谢和代谢性疾病、癌症、神经退行性疾病、衰老、卡路里限制和蛋白质折叠疾病的研究。

DESCRIPTION: We are requesting funds to purchase a Seahorse XF96 Extracellular Flux Analyzer, an instrument that profiles the metabolic activity and bioenergetic state of cells non-invasively, in minutes, and in a medium/high- throughput manner. The Seahorse XF96 Extracellular Flux Analyzer uses a simple microplate format to simultaneously measure oxygen consumption (cellular respiration) and extracellular acidification rates, in real time. Combined with the use of different substrates for energy and various inhibitors of specific enzymes and metabolic pathways, which can be delivered automatically via four integrated injection ports, the Seahorse Extracellular Flux Analyzer provides an unprecedented, thorough and fast assessment of oxidative phosphorylation, glycolysis, nutrient utilization, fatty acid oxidation, coupled vs. uncoupled respiration, and spare respiratory capacity of cells. There is currently no other single instrument that can perform the same range of metabolic assays in a 96-well format. Mitochondria function and proper substrate utilization are central to most physiologic processes important for human health, including growth, energy homeostasis, capacity for physical activity, and aging. Mitochondrial dysfunction has been implicated in the initiation, development and/or pathogenesis of a wide variety of diseases, including metabolic diseases (e.g. insulin resistance and development of type 2 diabetes), cancer, neurodegenerative diseases, and cardiovascular disease. The requested Seahorse XF96 Extracellular Flux Analyzer will enable a minimum of 12 investigators, which currently have no access to a similar instrument or technology, to gain insights into the cellular metabolism of a wide range of cells and organisms used to model physiological and pathological processes (including primary hepatocytes, adipocytes, neurons, cardiomyocytes, cancer cells, myotubes, and worms). The key elements of the Seahorse XF96 Extracellular Flux Analyzer that make it ideally suited for the specific needs of the users are the ability of the instrument to: 1) monitor attached cells (a crucial consideration for neurons, adipocytes, hepatocytes, and myotubes, used by 7 of 12 investigators); 2) obtain reliable measurements from small numbers of cells (crucial for 6 users that derive primary cells from mouse genetic models; and, 3) offer a medium/high throughput format, thereby enabling drug screening (5 projects) and evaluation of responses to a wide range of physiologic signals, environmental insults, and genetic alterations (important for all projects). The Seahorse XF96 Extracellular Flux Analyzer is unsurpassed in the combination of features that make it ideally suited to the studies of the current users. The instrument will be placed in a multi-user appropriate environment, readily accessible to all users, and available to new users. In summary, by providing a comprehensive assessment of cellular metabolism and mitochondrial function, the Seahorse XF96 Extracellular Flux Analyzer will facilitate and enhance our research on metabolism and metabolic diseases, cancer, neurodegenerative diseases, aging, calorie restriction, and diseases of protein folding.