Seahorse XF96 Extracellular Flux Analyzer

描述：我们正在申请资金购买一台Seahorse XF96细胞外通量分析仪，这是一种仪器，可以在几分钟内以中/高通量的方式无创地分析细胞的代谢活动和生物能量状态。海马XF96细胞外通量分析仪使用一个简单的微孔板格式，同时测量耗氧量（细胞呼吸）和细胞外酸化率，实时。结合使用不同底物的能量和特定酶和代谢途径的各种抑制剂，可以通过四个集成的注射口自动递送，海马细胞外通量分析仪提供了一个前所未有的，全面和快速的评估氧化磷酸化，糖酵解，营养利用，脂肪酸氧化，耦合与非耦合呼吸，以及细胞的备用呼吸能力。目前还没有其他单一仪器可以在96孔格式中执行相同范围的代谢分析。线粒体功能和适当的底物利用对大多数对人类健康重要的生理过程至关重要，包括生长、能量稳态、身体活动能力和衰老。线粒体功能障碍与多种疾病的发生、发展和/或发病机制有关，包括代谢性疾病（如胰岛素抵抗和2型糖尿病的发展）、癌症、神经退行性疾病和心血管疾病。所要求的Seahorse XF96细胞外通量分析仪将使至少12名研究人员能够深入了解用于模拟生理和病理过程的各种细胞和生物体的细胞代谢（包括原代肝细胞、脂肪细胞、神经元、心肌细胞、癌细胞、肌管和蠕虫），目前还没有类似的仪器或技术。海马XF96细胞外通量分析仪使其非常适合用户的特定需求的关键要素是该仪器能够：1)监测附着细胞（神经元，脂肪细胞，肝细胞和肌管的关键考虑因素，由12名研究人员中的7名使用）；2)从少量细胞中获得可靠的测量结果（对于从小鼠遗传模型中获得原代细胞的6个用户至关重要）；3)提供中/高通量格式，从而能够进行药物筛选（5个项目）和评估对各种生理信号、环境损害和遗传改变的反应（对所有项目都很重要）。海马XF96细胞外通量分析仪是无与伦比的功能组合，使其非常适合当前用户的研究。该仪器将放置在适合多用户使用的环境中，方便所有用户使用，并可供新用户使用。总之，通过提供细胞代谢和线粒体功能的综合评估，海马XF96细胞外通量分析仪将促进和加强我们对代谢和代谢性疾病、癌症、神经退行性疾病、衰老、热量限制和蛋白质折叠疾病的研究。

DESCRIPTION: We are requesting funds to purchase a Seahorse XF96 Extracellular Flux Analyzer, an instrument that profiles the metabolic activity and bioenergetic state of cells non-invasively, in minutes, and in a medium/high- throughput manner. The Seahorse XF96 Extracellular Flux Analyzer uses a simple microplate format to simultaneously measure oxygen consumption (cellular respiration) and extracellular acidification rates, in real time. Combined with the use of different substrates for energy and various inhibitors of specific enzymes and metabolic pathways, which can be delivered automatically via four integrated injection ports, the Seahorse Extracellular Flux Analyzer provides an unprecedented, thorough and fast assessment of oxidative phosphorylation, glycolysis, nutrient utilization, fatty acid oxidation, coupled vs. uncoupled respiration, and spare respiratory capacity of cells. There is currently no other single instrument that can perform the same range of metabolic assays in a 96-well format. Mitochondria function and proper substrate utilization are central to most physiologic processes important for human health, including growth, energy homeostasis, capacity for physical activity, and aging. Mitochondrial dysfunction has been implicated in the initiation, development and/or pathogenesis of a wide variety of diseases, including metabolic diseases (e.g. insulin resistance and development of type 2 diabetes), cancer, neurodegenerative diseases, and cardiovascular disease. The requested Seahorse XF96 Extracellular Flux Analyzer will enable a minimum of 12 investigators, which currently have no access to a similar instrument or technology, to gain insights into the cellular metabolism of a wide range of cells and organisms used to model physiological and pathological processes (including primary hepatocytes, adipocytes, neurons, cardiomyocytes, cancer cells, myotubes, and worms). The key elements of the Seahorse XF96 Extracellular Flux Analyzer that make it ideally suited for the specific needs of the users are the ability of the instrument to: 1) monitor attached cells (a crucial consideration for neurons, adipocytes, hepatocytes, and myotubes, used by 7 of 12 investigators); 2) obtain reliable measurements from small numbers of cells (crucial for 6 users that derive primary cells from mouse genetic models; and, 3) offer a medium/high throughput format, thereby enabling drug screening (5 projects) and evaluation of responses to a wide range of physiologic signals, environmental insults, and genetic alterations (important for all projects). The Seahorse XF96 Extracellular Flux Analyzer is unsurpassed in the combination of features that make it ideally suited to the studies of the current users. The instrument will be placed in a multi-user appropriate environment, readily accessible to all users, and available to new users. In summary, by providing a comprehensive assessment of cellular metabolism and mitochondrial function, the Seahorse XF96 Extracellular Flux Analyzer will facilitate and enhance our research on metabolism and metabolic diseases, cancer, neurodegenerative diseases, aging, calorie restriction, and diseases of protein folding.