Functions of conditional Cx36-dependent electrical synapses in the neocortex

产品说明：皮质节律与注意力、探索和导航行为以及精神分裂症等疾病状态有关，但其机制仍不清楚。它们起源的一个线索是，神经元间隙连接表达是在神经元中获得全功率所必需的。 海马体中的伽马带间隙连接使相邻的相同亚型的抑制性中间神经元同步，但它们在网络活动中的作用仍然知之甚少。我建议研究两个间隙连接的网络的中间神经元，生长抑素表达的中间神经元和小白蛋白表达的中间神经元，和两种类型的皮质振荡，θ（4-12赫兹）和γ（30-80赫兹）之间的相互作用。我将通过选择性地消除两个神经元间网络中基于连接蛋白36的间隙连接，并通过全细胞记录和体内脑电图和层状电极阵列测量体外产生的γ和θ节律的后果来研究这种联系。

DESCRIPTION: Cortical rhythms have been implicated in attention, exploratory and navigatory behavior, and in disease states such as schizophrenia, but their mechanisms remain vague. One clue to their origin is that neuronal gap junction expression is required for full power in the gamma band in the hippocampus. Gap junctions synchronize neighboring inhibitory interneurons of the same subtype but their role in network activity remains poorly understood. I propose to investigate the interactions between two gap-junction connected networks of interneurons, somatostatin-expressing interneurons and parvalbumin-expressing interneurons, and two types of cortical oscillations, theta (4-12 Hz) and gamma (30-80 Hz). I will investigate this link by selectively eliminating connexin36-based gap junctions in the two interneuronal networks and measuring the consequences on gamma and theta rhythms generated in vitro with whole-cell recordings and in vivo via electroencephalography and laminar electrode arrays.