BK Channels of Hair Cells

描述（由申请人提供）：该提案旨在以小鼠和鸡毛细胞为模型系统，对听力重要的大电导钙活化钾（BK）通道如何影响毛细胞功能进行分子理解。该方案旨在确定BK通道如何定位于毛细胞的基底外侧表面。特别是，该提案旨在确定Slo在毛细胞中的运输是如何发生的，并验证了毛细胞中的Slo在极化上皮细胞中使用基底外侧分泌途径的假设。将探讨磷酸化和相关蛋白在基底外侧分选中的作用。该项目还将确定剪接、磷酸化和Slo相关蛋白如何影响BK动力学，试图将这些变化与天然毛细胞中的变化联系起来。

DESCRIPTION (provided by applicant): The proposal seeks to bring a molecular understanding of how large conductance calcium activated potassium (BK) channels, which are important for hearing affect hair cell function using mouse and chicken hair cells as a model system. The channels consist of the Slo protein and a variable number of associated proteins The proposal seeks to determine how BK channels are localized at the basolateral surface of hair cells. In particular the proposal seeks to ascertain how Slo trafficking in hair cells occur ad tests the hypothesis that Slo in hair cells uses the basolateral secretory pathway in polarized epithelial cells. The role of phosphorylation and associated proteins in bringing about basolateral sorting will be explored. The project will also determine how splicing, phosphorylation and Slo associated proteins affect BK kinetics attempting to relate these changes to those in native hair cells.