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BK Channels of Hair Cells

毛细胞的 BK 通道

基本信息

批准号：
8448046
负责人：
DHASAKUMAR S NAVARATNAM
金额：
$ 35.34万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-07-01 至 2018-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8448046
关键词：
Ablation Affect Age Alternative Splicing Animals Apical Biochemical Biological Models CDK5 gene Calcium Cell Line Cell membrane Cell physiology Cells Characteristics Chickens Cyclic AMP-Dependent Protein Kinases Data Disease Endosomes Epilepsy Epithelial Cells Epithelium Exons Frequencies Funding Golgi Apparatus Hair Cells Hearing Hypertension Individual Kinetics Knockout Mice Label Lateral MDCK cell Mammals Mediating Membrane Methods Molecular Mus Optics Organelles Outer Hair Cells Output Pathway interactions Phosphorylation Phosphorylation Site Phosphotransferases Physiological Play Potassium Property Protein Kinase Proteins RNA Splicing Recycling Role S-nitro-N-acetylpenicillamine Small Interfering RNA Sorting - Cell Movement Study models Surface Testing Time Vertebrates base beta catenin cholinergic density established cell line hearing impairment inhibitor/antagonist large-conductance calcium-activated potassium channels mutant public health relevance receptor reconstitution response trafficking vector voltage

项目摘要

DESCRIPTION (provided by applicant): The proposal seeks to bring a molecular understanding of how large conductance calcium activated potassium (BK) channels, which are important for hearing affect hair cell function using mouse and chicken hair cells as a model system. The channels consist of the Slo protein and a variable number of associated proteins The proposal seeks to determine how BK channels are localized at the basolateral surface of hair cells. In particular the proposal seeks to ascertain how Slo trafficking in hair cells occur ad tests the hypothesis that Slo in hair cells uses the basolateral secretory pathway in polarized epithelial cells. The role of phosphorylation and associated proteins in bringing about basolateral sorting will be explored. The project will also determine how splicing, phosphorylation and Slo associated proteins affect BK kinetics attempting to relate these changes to those in native hair cells.

描述(由申请人提供)：该提案试图以小鼠和鸡毛细胞为模型系统，对大电导钙激活钾(BK)通道如何影响毛细胞功能进行分子理解，BK通道对听力非常重要。通道由Slo蛋白和数量可变的相关蛋白组成，该提案试图确定BK通道是如何定位在毛细胞的基底外侧表面的。特别是，该提案试图确定Slo在毛细胞中的运输是如何发生的，并测试了毛细胞中的Slo使用极化上皮细胞的基侧分泌途径的假设。本课程将探讨磷酸化及相关蛋白质在实现基侧分选中的作用。该项目还将确定剪接、磷酸化和Slo相关蛋白如何影响BK动力学，试图将这些变化与天然毛细胞中的变化联系起来。