Histology

组织学

• 批准号: 8733296
• 负责人: CHERYL F DREYFUS
• 金额: $ 7.03万
• 依托单位: RBHS-ROBERT WOOD JOHNSON MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-03 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8733296
• 关键词: Antibodies; Brain; Core Facility; Evaluation; Growth; Histology; Molecular; Nervous system structure; Paraffin; Procedures; Protocols documentation; Quality Control; Savings; Services; Slice; Staining method; Stains; cryostat; tissue preparation

The Histology Core will function to provide extensive services to each of the five projects. The Core Facility will be used in 4 ways. First, it will optimize staining protocols for antibodies used for evaluation of specific markers in sections from brain, slice cultures and dissociated cultures. Second, it will provide sectioning (paraffin or cryostat). Third, it will perform immunohistochemisty. Fourth, it will provide quantitative stereological services to projects. Thus, it will host a complete range of services for all projects, with oversight by the Pis and technical support staff to standardize procedures and maintain quality control. This will result in significant savings in terms of immunohistochemical supplies and in tissue preparation and sectioning.

组织学核心将为这五个项目中的每一个项目提供广泛的服务。核心 设施将以 4 种方式使用。首先，它将优化用于评估的抗体的染色方案 大脑切片、切片培养物和解离培养物中的特定标记。其次，它将提供 切片（石蜡或低温切片机）。第三，它将进行免疫组织化学分析。第四，提供定量的 为项目提供体视服务。因此，它将为所有项目提供全方位的服务，并进行监督 由 Pis 和技术支持人员负责标准化程序并保持质量控制。这将导致 显着节省免疫组织化学用品以及组织制备和切片方面的费用。