THE NINDS MASS SPECTROMETRY PROTEOMICS CENTER FOR NEUROSCIENCES AT MSSM

神经科学中的一个主要科学挑战是在分子水平上识别和表征各种生理条件下的细胞变化和病理性神经疾病。这一努力取得成功的关键是开发和实施新的工具，用于快速和灵敏地鉴定蛋白质及其翻译后修饰的特征。高灵敏度的质谱学已成为此类研究的宝贵工具。该中心的主要目标是建立一个核心设施，配备专职人员支持NINDS资助的神经科学研究项目，扩大和提升我们的质谱学设施的容量和灵敏度，将最先进的质谱学技术应用于神经科学和神经系统疾病研究中涉及的关键蛋白质和神经肽的分析，并支持和加强西奈山的神经科学研究计划。这一核心设施的具体目标是：(1)为NINDS和西奈山的其他神经科学研究人员以及其他研究所的NINDS资助的其他研究人员提供快速、经济高效和高质量的质谱学蛋白质组服务，以便进行尖端研究，其中使用新的质谱学技术来解决重要的生化和生物医学问题。(2)将新开发的质谱学技术从我的研究实验室转移到NINDS核心设施，并扩大能力和提高质谱学技术的灵敏度，以帮助NINDS资助的研究人员和其他面向神经科学的研究人员。(3)教育NINDS资助的研究人员、其他面向神经科学的科学家及其工作人员在神经科学中使用质谱学进行蛋白质分析。(4)激发新的研究主动性，促进神经科学领域的合作。

A major scientific challenge in neuroscience is the identification and characterization at molecular level of the cellular changes in various physiological conditions and pathological neurological disorders. Critical to the success of this endeavor is the development and implementation of new tools for rapid and sensitive protein identification and characterization of their post-translational modifications. High sensitivity of mass spectrometry has become an invaluable tool for such studies. The major goal of the proposed "MINDS Mass Spectrometry Proteomics Core Facility for Neurosciences at MSSM" is to establish a core facility with dedicated personnel in supporting NINDS-funded neuroscience research programs, to expand and upgrade the capacity and sensitivity of our mass spectrometry facility, to apply state-of-the-art mass spectrometry technologies to critical protein and neuropeptide analysis involved in neuroscience and neurological disease research, and to enable and strengthen neuroscience research program at Mount Sinai. The specific aims of this core facility are: (1) To provide rapid, cost-effective, and high-quality mass spectrometry proteomics service for NINDS and other neuroscience-oriented investigators at Mount Sinai and other NINDS-funded investigators in other institute in order to perform cutting-edge research in which new mass spectrometric technologies are used to solve important biochemical and biomedical problems. (2) To transfer newly developed mass spectrometry technology from my research laboratory to the NINDS Core Facility and expand the capacity and upgrade the sensitivity of mass spectrometry techniques for assisting NINDS-funded and other neuroscience-oriented investigators. (3) To educate NINDS-funded investigators, other neuroscience-oriented scientists, and their staffs on using mass spectrometry for protein analysis in neuroscience. (4) To stimulate new research initiatives and facilitate collaborations in neuroscience.