THE NINDS MASS SPECTROMETRY PROTEOMICS CENTER FOR NEUROSCIENCES AT MSSM

神经科学中的一个主要科学挑战是在分子水平上识别和表征各种生理条件和病理性神经系统疾病中的细胞变化。这一奋进取得成功的关键是开发和实施新的工具，用于快速和灵敏的蛋白质鉴定及其翻译后修饰的表征。质谱的高灵敏度已成为此类研究的宝贵工具。拟议的“MSSM神经科学MINDS质谱蛋白质组学核心设施”的主要目标是建立一个核心设施，配备专门人员支持NINDS资助的神经科学研究项目，扩大和升级我们质谱设施的容量和灵敏度，为了将最先进的质谱技术应用于神经科学和神经疾病研究中涉及的关键蛋白质和神经肽分析，并支持和加强西奈山的神经科学研究计划。该核心设施的具体目标是：（1）为NINDS和西奈山其他神经科学研究人员以及其他NINDS资助的研究人员提供快速，具有成本效益和高质量的质谱蛋白质组学服务，以便进行尖端研究，其中新的质谱技术用于解决重要的生物化学和生物医学问题。(2)将新开发的质谱技术从我的研究实验室转移到NINDS核心设施，并扩大容量和升级质谱技术的灵敏度，以协助NINDS资助的和其他面向神经科学的研究人员。(3)教育NINDS资助的研究人员，其他神经科学导向的科学家，以及他们的工作人员在神经科学中使用质谱法进行蛋白质分析。(4)刺激新的研究计划，促进神经科学的合作。

A major scientific challenge in neuroscience is the identification and characterization at molecular level of the cellular changes in various physiological conditions and pathological neurological disorders. Critical to the success of this endeavor is the development and implementation of new tools for rapid and sensitive protein identification and characterization of their post-translational modifications. High sensitivity of mass spectrometry has become an invaluable tool for such studies. The major goal of the proposed "MINDS Mass Spectrometry Proteomics Core Facility for Neurosciences at MSSM" is to establish a core facility with dedicated personnel in supporting NINDS-funded neuroscience research programs, to expand and upgrade the capacity and sensitivity of our mass spectrometry facility, to apply state-of-the-art mass spectrometry technologies to critical protein and neuropeptide analysis involved in neuroscience and neurological disease research, and to enable and strengthen neuroscience research program at Mount Sinai. The specific aims of this core facility are: (1) To provide rapid, cost-effective, and high-quality mass spectrometry proteomics service for NINDS and other neuroscience-oriented investigators at Mount Sinai and other NINDS-funded investigators in other institute in order to perform cutting-edge research in which new mass spectrometric technologies are used to solve important biochemical and biomedical problems. (2) To transfer newly developed mass spectrometry technology from my research laboratory to the NINDS Core Facility and expand the capacity and upgrade the sensitivity of mass spectrometry techniques for assisting NINDS-funded and other neuroscience-oriented investigators. (3) To educate NINDS-funded investigators, other neuroscience-oriented scientists, and their staffs on using mass spectrometry for protein analysis in neuroscience. (4) To stimulate new research initiatives and facilitate collaborations in neuroscience.