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Core Facilities for Neurobiology at Brandeis

布兰迪斯神经生物学核心设施

基本信息

批准号：
8387998
负责人：
MICHAEL ROSBASH
金额：
$ 75.56万
依托单位：
BRANDEIS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-09-30 至 2014-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8387998
关键词：
Address Alzheimer&apos s Disease Amyotrophic Lateral Sclerosis Autistic Disorder Behavior Biological Models Brain Cell physiology Communities Computational Biology Core Facility Disease Electron Microscopy Ensure Epilepsy Faculty Funding Gene Transfer Techniques Genomics High Performance Computing Image Invertebrates Lentivirus Vector Light Mental disorders Moods National Institute of Neurological Disorders and Stroke Neurobiology Neurodegenerative Disorders Neurodevelopmental Disorder Neurons Neurosciences Proteomics Research Project Grants Rett Syndrome Schizophrenia Science Short-Term Memory Sleep disturbances Synaptic Transmission Transfection Transgenic Animals Transgenic Mice Transgenic Mouse Facility Universities Viral fluorescence imaging interest light microscopy member nervous system disorder neural circuit relating to nervous system simulation ultra high resolution

项目摘要

This is a proposal to continue NINDS funding of a Core Facility at Brandeis University. This will allow us to fully exploit the three subcomponents that are now firmly established: a microarray and FACS facility, an imaging facility and a mouse/transgenic facility. They are the underpinnings of a large number of NINDS- funded and other neuroscience-relevant research projects on campus. We also propose major expansions of all three existing cores and the establishment of a fourth core. The microarray/FACS facility will be expanded to encompass proteomics thereby becoming the Genomics/Proteomics Core Facility. The imaging facility will be expanded to include Correlated Light and Electron Microscopy (CLEM) and ultra high- resolution cryo-fluorescence imaging thereby become the Imaging/CLEM Core Facility. The mouse/transgenic facility has recently added the capacity to produce Lentiviral vectors for transfection and transgenesis, and is now referred to as the Transgenic Mouse and Viral Transfection Core Facility. Finally, we will establish a new Computational Core Facility to support large scale neural simulations and computational biology projects as part of a large high-performance computing cluster. These additions will allow the Brandeis community to remain at the cutting edge technologically, and ensure that we can continue to generate exciting and ground-breaking new science. The projects supported by the cores are joined together through the shared interests of multiple neuroscience faculty members in basic as well disease- related aspects of brain and neuron function: cell identity, synaptic transmission and circuits, plasticity, behavior and its modulation. The proposed studies will exploit vertebrate and invertebrate model systems, with a strong emphasis on transgenic animals. They address basic and applied problems that are pertinent to a wide range of neurological and psychiatric diseases including disturbances of excitability, such as epilepsy, disturbances of sleep, waking and mood, neurodevelopmental disorders such as Autism and Rett Syndrome, neurodegenerative disorders, like Amyotrophic Lateral Sclerosis, and disturbances of long and short -term memory such as those that accompany Alzheimer's Disease and Schizophrenia.

这是一项继续为布兰代斯大学的核心基金提供NINDS资金的提案。这将使我们能够充分利用现在稳固建立的三个子组成部分：微阵列和FACS设施、成像设施和小鼠/转基因设施。它们是NINDS资助的大量校园和其他与神经科学相关的研究项目的基础。我们还建议对现有的所有三个核心进行重大扩展，并建立第四个核心。微阵列/流式细胞仪设施将扩大到涵盖蛋白质组学，从而成为基因组学/蛋白质组学核心设施。成像设施将扩大到包括相关光和电子显微镜(CLEM)和超高分辨率低温荧光成像，从而成为成像/CLEM核心设施。小鼠/转基因设施最近增加了生产用于转基因和转基因的慢病毒载体的能力，现在被称为转基因小鼠和病毒转染核心设施。最后，我们将建立一个新的计算核心设施，作为大型高性能计算集群的一部分，支持大规模神经模拟和计算生物学项目。这些增加将使Brandeis社区保持在技术前沿，并确保我们能够继续产生令人兴奋和突破性的新科学。这些核心支持的项目通过多名神经科学教员在大脑和神经元功能的基本方面以及与疾病相关的方面的共同兴趣而结合在一起：细胞识别、突触传输和电路、可塑性、行为及其调节。拟议的研究将利用脊椎动物和无脊椎动物的模型系统，重点是转基因动物。它们解决与广泛的神经和精神疾病有关的基本和应用问题，包括兴奋性障碍，如癫痫，睡眠、清醒和情绪障碍，神经发育障碍，如自闭症和雷特综合症，神经退行性疾病，如肌萎缩侧索硬化症，以及长期和短期记忆障碍，如阿尔茨海默氏症和精神分裂症。

项目成果

期刊论文数量（20）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Unique transposon landscapes are pervasive across Drosophila melanogaster genomes.

DOI：
10.1093/nar/gkv1193
发表时间：
2015-12-15
期刊：
Nucleic acids research
影响因子：
14.9
作者：
Rahman R;Chirn GW;Kanodia A;Sytnikova YA;Brembs B;Bergman CM;Lau NC
通讯作者：
Lau NC

Conserved piRNA Expression from a Distinct Set of piRNA Cluster Loci in Eutherian Mammals.

DOI：
10.1371/journal.pgen.1005652
发表时间：
2015-11
期刊：
PLoS genetics
影响因子：
4.5
作者：
Chirn GW;Rahman R;Sytnikova YA;Matts JA;Zeng M;Gerlach D;Yu M;Berger B;Naramura M;Kile BT;Lau NC
通讯作者：
Lau NC

p75 and TrkA signaling regulates sympathetic neuronal firing patterns via differential modulation of voltage-gated currents.

DOI：
10.1523/jneurosci.3503-08.2009
发表时间：
2009-04-29
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
0
作者：
Luther JA;Birren SJ
通讯作者：
Birren SJ

CaMKI-dependent regulation of sensory gene expression mediates experience-dependent plasticity in the operating range of a thermosensory neuron.

DOI：
10.1016/j.neuron.2014.10.046
发表时间：
2014-12-03
期刊：
NEURON
影响因子：
16.2
作者：
Yu, Yanxun V.;Bell, Harold W.;Glauser, Dominique A.;Van Hooser, Stephen D.;Goodman, Miriam B.;Sengupta, Piali
通讯作者：
Sengupta, Piali

The capacity of target silencing by Drosophila PIWI and piRNAs.