The Role of Interleukin-18 in Myocardial Hypertrophy and Failure
The Role of Interleukin-18 in Myocardial Hypertrophy and Failure
批准号:
8434206
负责人:
Chandrasekar Bysani
金额:
$35.11万
依托单位国家:
美国
项目类别:
财政年份:
2008
资助国家:
美国
项目状态:
已结题
起止时间:
2008-12-15 至 2015-02-28
关键词:
70-kDa Ribosomal Protein S6 KinasesActivation AnalysisAddressAdhesionsAttenuatedBiochemicalCardiacCardiac MyocytesCessation of lifeChronicClinicalCongestive Heart FailureDataDiseaseDown-RegulationExhibitsExtracellular MatrixFailureFibroblastsFibrosisFunctional disorderGene ExpressionGenesGoalsGrowth FactorGrowth Factor GeneHealthHeartHeart HypertrophyHeart failureHospitalizationHumanHypertrophyImmuneIn VitroInflammation MediatorsInflammatoryInflammatory ResponseInterleukin-18InterleukinsInvestigationKnockout MiceLeft Ventricular HypertrophyLinkMatrix Metalloproteinase InhibitorMatrix MetalloproteinasesMeasuresMediatingMediator of activation proteinModelingMolecularMorbidity - disease rateMusMuscle CellsMyocardialOryctolagus cuniculusOutcomePTEN genePathologic ProcessesPathologyPathway interactionsPatient CarePatientsPhase TransitionPhenotypePhosphotransferasesPlayProcessProductionPublishingRegulationRelative (related person)ReportingRiskRoleSERCA2aSerumSeveritiesSeverity of illnessSignal TransductionSignal Transduction PathwaySignaling MoleculeStressSudden DeathTestingTherapeuticTissuesTransgenic MiceTransgenic OrganismsWild Type Mousebasechemokineconstrictioncytokinedesignfetalgain of functionhuman TSC2 proteinin vivoinnovationinterleukin-18 binding proteininterleukin-18 receptorloss of functionmigrationmortalitymouse modelneutralizing antibodynoveloverexpressionpressureprognosticresponsetherapeutic target
中文摘要
描述(由申请人提供):心肌肥厚及其向衰竭的转变仍然是发病率和死亡率的重要原因。炎性细胞因子的持续产生是这一转变的所有阶段的标志。尤其是白介素18在心力衰竭中表达上调,与心力衰竭患者心肌损伤和功能障碍的严重程度及不良的临床结局直接相关。我们的初步研究表明,IL-18在体外诱导心肌细胞肥大和成纤维细胞迁移和增殖,提示IL-18在体内具有潜在的促肥大和促纤维化作用。我们在野生型小鼠身上的研究表明,横动脉缩窄(TAC)引起的压力超负荷会导致左心室肥厚(LVH)和IL-18表达增加。值得注意的是,这种肥大可以被IL-18中和抗体显著减轻。IL-18基因敲除小鼠对TAC的反应显著减少了左心室肥厚;相反,心脏特异的IL-18过表达在没有TAC的情况下诱导左心室肥厚和心力衰竭。兔模型也表现出左室肥厚和IL-18表达增加作为对TAC的反应。此外,我们的初步人体研究清楚地证明了系统性IL-18水平预测心力衰竭的能力。因此,我们的中心假设是,IL-18是左心室肥厚和衰竭的关键介质,通过诱导肥厚相关蛋白、胎儿基因、生长因子和基质金属蛋白酶而导致病理性重构。为了解决这一假设,我们将使用心脏限制性IL-18KO和心脏特异性IL-18转基因小鼠(特异性目标3),研究体外心肌细胞中IL-18依赖的信号转导(特异性目标1),参与IL-18介导的心脏成纤维细胞迁移和增殖的分子机制(特异性目标2),以及IL-18在体内左室肥厚、纤维化和衰竭中的致病作用(特异性目标3)。在小鼠身上获得的结果将在压力超负荷肥大和衰竭的兔模型中得到验证。系统的IL-18水平将被测量,并与人类心脏肥厚和衰竭的相对严重程度相关。总之,这些拟议的研究将确立IL-18作为一种潜在的治疗靶点,以减缓左室肥厚向心力衰竭的进展。
英文摘要
DESCRIPTION (provided by applicant): Myocardial hypertrophy and its transition to failure remains a significant cause of morbidity and mortality. Sustained production of inflammatory cytokines is a hallmark of all phases of this transition. In particular, interleukin (IL)-18 is upregulated in heart failure, which directly correlates with the severity of myocardial damage and dysfunction, and poor clinical outcome in heart failure. Our preliminary studies demonstrate that IL-18 induces cardiomyocyte hypertrophy and fibroblast migration and proliferation in vitro, suggesting potential pro-hypertrophic and pro-fibrotic roles for IL-18 in vivo. Our studies in wild-type mice show that pressure overload induced by transverse aortic constriction (TAC) leads to left ventricular hypertrophy (LVH) and increased IL-18 expression. Remarkably, this hypertrophy can be significantly reduced by IL-18 neutralizing antibodies. IL-18 knockout mice develop significantly less LVH in response to TAC; conversely, cardiac-specific overexpression of IL-18 induces LVH and heart failure in the absence of TAC. Rabbit models also exhibit LVH and increased IL-18 expression in response to TAC. Furthermore, our preliminary human studies clearly demonstrate the prognostic power of systemic IL-18 levels to predict cardiac failure. Thus, our central HYPOTHESIS is that IL-18 is a key mediator of LVH and failure that results in pathological remodeling through the induction of hypertrophy-associated kinases, fetal genes, growth factors, and matrix metalloproteinases. To address this HYPOTHESIS, we will investigate IL-18-dependent signaling in cardiomyocytes in vitro (Specific Aim 1), the molecular mechanisms involved in IL-18-mediated cardiac fibroblast migration and proliferation in vitro (Specific Aim 2), and the causal role of IL-18 in LVH, fibrosis and failure in vivo, using cardiac-restricted IL-18KO and cardiac-specific IL-18 transgenic mice (Specific Aim 3). Results obtained in mice will be validated in a rabbit model of pressure-overload hypertrophy and failure. Systemic IL-18 levels will be measured and correlated with the relative severity of cardiac hypertrophy and failure in humans. Collectively, these proposed studies will establish IL-18 as a potentially use therapeutic target to attenuate the progression of LVH to cardiac failure.
期刊论文(7)
专著(0)
科研奖励(0)
会议论文
登录
查看更多内容
OxLDL induces endothelial dysfunction and death via TRAF3IP2: inhibition by HDL3 and AMPK activators.
OXLDL通过TRAF3IP2诱导内皮功能障碍和死亡:HDL3和AMPK激活剂的抑制作用。
DOI:
10.1016/j.freeradbiomed.2014.02.014
发表时间:
2014-05
期刊:
FREE RADICAL BIOLOGY AND MEDICINE
影响因子:
7.4
作者:
[Valente, Anthony J., Irimpen, Anand M., Siebenlist, Ulrich, Chandrasekar, Bysani]
通讯作者:
Chandrasekar, Bysani
DOI:
10.1016/j.yjmcc.2012.04.009
发表时间:
2012-07
期刊:
Journal of molecular and cellular cardiology
影响因子:
5
作者:
[Valente AJ, Clark RA, Siddesha JM, Siebenlist U, Chandrasekar B]
通讯作者:
Chandrasekar B
Periodontal disease in association with systemic levels of interleukin-18 and CXC ligand 16 in patients undergoing cardiac catheterization.
接受心导管插入术的患者中,牙周病与白细胞介素 18 和 CXC 配体 16 的全身水平相关。
DOI:
10.1902/jop.2010.100046
发表时间:
2010
期刊:
Journal of periodontology
影响因子:
4.3
作者:
[Schallhorn,RachelA, Patel,DevangN, Chandrasekar,Bysani, Mealey,BrianL]
通讯作者:
Mealey,BrianL
DOI:
10.1016/j.cellsig.2013.07.013
发表时间:
2013-11
期刊:
Cellular signalling
影响因子:
4.8
作者:
[Valente AJ, Sakamuri SS, Siddesha JM, Yoshida T, Gardner JD, Prabhu R, Siebenlist U, Chandrasekar B]
通讯作者:
Chandrasekar B
DOI:
10.1016/j.yjmcc.2013.09.015
发表时间:
2013-12
期刊:
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
影响因子:
5
作者:
[Siddesha, Jalahalli M., Valente, Anthony J., Sakamuri, Siva S. V. P., Yoshida, Tadashi, Gardner, Jason D., Somanna, Naveen, Takahashi, Chiaki, Noda, Makoto, Chandrasekar, Bysani]
通讯作者:
Chandrasekar, Bysani
Role of novel RNA binding protein LARP6 in alcoholic cardiomyopathy
-
批准号:10593688
-
项目类别:
-
资助金额:$23.09万
-
财政年份:2023
-
负责人:Chandrasekar Bysani
-
依托单位:
RECK in Adverse Cardiac Remodeling and Heart Failure
-
批准号:10368301
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2022
-
负责人:Chandrasekar Bysani
-
依托单位:
RECK in Adverse Cardiac Remodeling and Heart Failure
-
批准号:10655310
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2022
-
负责人:Chandrasekar Bysani
-
依托单位:
RECK regulation of NASH and fibrosis
-
批准号:10616763
-
项目类别:
-
资助金额:$55.19万
-
财政年份:2022
-
负责人:Chandrasekar Bysani
-
依托单位:
TRAF3IP2 in Adverse Cardiac Remodeling and Heart Failure
-
批准号:10266002
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2018
-
负责人:Chandrasekar Bysani
-
依托单位:
BLR&D Research Career Scientist Award Application
-
批准号:10047289
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2017
-
负责人:Chandrasekar Bysani
-
依托单位:
BLRD Research Career Scientist Award Application
-
批准号:10587293
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2017
-
负责人:Chandrasekar Bysani
-
依托单位:
BLR&D Research Career Scientist Award Application
-
批准号:10293563
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2017
-
负责人:Chandrasekar Bysani
-
依托单位:
TRAF3IP2 in Ischemic Heart Disease
-
批准号:9230762
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2014
-
负责人:Chandrasekar Bysani
-
依托单位:
TRAF3IP2 in Ischemic Heart Disease
-
批准号:9339531
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2014
-
负责人:Chandrasekar Bysani
-
依托单位:
TRAF3IP2 in Ischemic Heart Disease
-
批准号:8846473
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2014
-
负责人:Chandrasekar Bysani
-
依托单位:
TRAF3IP2 in Ischemic Heart Disease
-
批准号:8736118
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2014
-
负责人:Chandrasekar Bysani
-
依托单位:
Interleukin-18 and post infarct myocardial remodeling
-
批准号:7784475
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2009
-
负责人:Chandrasekar Bysani
-
依托单位:
Interleukin-18 and post infarct myocardial remodeling
-
批准号:8206292
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2009
-
负责人:Chandrasekar Bysani
-
依托单位:
Interleukin-18 and post infarct myocardial remodeling
-
批准号:7686633
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2009
-
负责人:Chandrasekar Bysani
-
依托单位:
Interleukin-18 and post infarct myocardial remodeling
-
批准号:8394594
-
项目类别:
-
资助金额:$0.0万
-
财政年份:2009
-
负责人:Chandrasekar Bysani
-
依托单位:
The Role of Interleukin-18 in Myocardial Hypertrophy and Failure
-
批准号:7750524
-
项目类别:
-
资助金额:$37.25万
-
财政年份:2008
-
负责人:Chandrasekar Bysani
-
依托单位:
The role of interleukin-18 in myocardial hypertrophy and failure
-
批准号:7582858
-
项目类别:
-
资助金额:$34.58万
-
财政年份:2008
-
负责人:Chandrasekar Bysani
-
依托单位:
The Role of Interleukin-18 in Myocardial Hypertrophy and Failure
-
批准号:8230543
-
项目类别:
-
资助金额:$36.88万
-
财政年份:2008
-
负责人:Chandrasekar Bysani
-
依托单位:
Chemokines, nitric oxide, and myocardial depression
-
批准号:6640325
-
项目类别:
-
资助金额:$25.14万
-
财政年份:2002
-
负责人:Chandrasekar Bysani
-
依托单位:
海外基金