cell Function & Pathophysiology Project

细胞功能

• 批准号: 8294095
• 负责人: Ted M. Dawson
• 金额: $ 18.41万
• 依托单位: MCLEAN HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8294095
• 关键词: Affect; AlamarBlue; Amphetamines; Apomorphine; Axon; Biogenesis; Biological; Biological Assay; Calcium; Cell Line; Cell physiology; Cells; Cellular Morphology; Clinical Investigator; Complement; Confocal Microscopy; Cytosol; Data; Dendrites; Disease; Dopamine; Dose; Dyes; Environment; Enzyme-Linked Immunosorbent Assay; Evaluation; Free Radicals; Functional disorder; Gene Mutation; Gene Targeting; Generations; Genes; Genotype; Genus Hippocampus; Glucose; Human; Image; Immunohistochemistry; In Vitro; Individual; Investigation; Ion Channel; Ions; LRRK2 gene; Laboratories; Lewy Bodies; Life; Link; Measurement; Measures; Membrane Potentials; Mitochondria; Mitochondrial Matrix; Modeling; Monitor; Movement; Mus; Mutation; Neurites; Neurons; Other Genetics; Oxidation-Reduction; PINK1 gene; Pathogenesis; Pathology; Pathway interactions; Phenotype; Phosphorylation; Physiological; Play; Population; Preclinical Drug Evaluation; Process; Proteins; Rattus; Reactive Oxygen Species; Recruitment Activity; Reporter; Research; Respiration; Reverse Transcriptase Polymerase Chain Reaction; Role; Scientist; Screening procedure; Seeds; Shapes; Side; Staining method; Stains; Structure; Techniques; Toxin; Transmission Electron Microscopy; Transplantation; Western Blotting; Work; adeno-associated viral vector; aged; alpha synuclein; base; cellular imaging; cytotoxicity; dopaminergic neuron; glucose metabolism; immunocytochemistry; in vivo; in vivo Bioassay; induced pluripotent stem cell; meetings; member; mitochondrial membrane; neuronal cell body; novel strategies; parkin gene/protein; patch clamp; receptor; relating to nervous system; synaptogenesis

The Cell Function and Pathophysiology Core will be led by Dr. Ted Dawson and will function to determine disease-associated phenotypes from the differentiated PDiPS cell lines. In addition, the members of the Cell Function and Pathophysiology Core will be responsible for developing the existing phenotypic assays into a format amenable for large-scale drug screening. In conclusion, we think that the talented clinical investigators and translational scientists that comprise our current PDiPS Consortium are well placed to transition our groundbreaking work into a U24 format that meets the criteria outlined in the new RFA (RFA-NS-11-011, see Fig. 3 for Milestones).

细胞功能和病理生理学核心小组将由泰德·道森博士和威尔