ANIMAL CORE

动物核心为研究人员提供了慢性仪器化的绵羊和非仪器化的怀孕和未怀孕的动物。动物核心的具体目标是：在怀孕75天时获得有时间日期的怀孕动物；准备用于不同研究项目的长期测量仪器的幼羊和成年羊；进行身体解剖和便利组织收集，并促进涉及监测急性或慢性条件下的生理变量的活体研究。怀孕的母羊被随机分配到怀孕80天和81天时接受赋形剂或贝塔美松注射，羔羊被允许在足月自然分娩。 动物核心对于其中三个研究项目是必不可少的。这位核心主任有超过25年的工作经验，处理长期使用仪器的胎儿和怀孕动物。该核心提供了独特的组合能力，能够注入生理剂量的推定调节剂，可获得血浆样本，在体内记录生理变量，以及从同一动物体内进行体外研究所需的组织。 绵羊将被长期测量，以获得不处于麻醉压力下的动物的生理数据。我们将使用Hash，Inc.提供给我们的过期怀孕绵羊。该供应保持着非常大的羊群，并一直为我们提供健康和准确日期的怀孕动物。所有这些手术和操作都将在围产期研究实验室的动物核心内进行。怀孕和成年的绵羊将被使用，无论性别。倍他米松的给药方式类似于用来刺激胎儿肺成熟的方式，即两剂醋酸盐和磷酸盐的混合物，相隔24小时。唯一不同的是，我们将根据孕妇的体重进行调整，每天给予0.17毫克/公斤，最高12毫克/天。

The Animal Core provides the investigators with chronically instrumented sheep and with non instrumented pregnant and nonpregnant animals. The specific goals of the Animal Core are: to obtain time- dated pregnant animals at 75 days of gestation; to prepare chronically instrumented juvenile and adult sheep for use in the different research projects; to conduct necropsies and facilitate the collection of tissues and to facilitate the in vivo studies involving monitoring physiologic variables under acute or chronic conditions. Pregnant ewes are randomly assigned to receive either vehicle or beta methasone injections at 80 an 81 days gestation and lambs are allowed to deliver spontaneously at term. The Animal Core is essential for three of the research projects. The director of the core has more than 25 years of experience working with chronically instrumented fetal and pregnant animals. The Core provides the unique combination of the ability to infuse physiological amounts of putative regulators, the availability of plasma samples, in vivo recording of physiological variables and tissues for in vitro studies from the same animal in which in vivo studies have been conducted. Sheep will be chronically instrumented in order to obtain physiological data in animals that are not under the stress of anesthesia. We will use time dated pregnant sheep provided to us by Hash, Inc. The supplies maintains a very large flock and has consistently provided us with healthy and accurately dated pregnant animals. All of these surgeries and manipulations will be conducted within the Animal Core in the Perinatal Research Laboratory. Pregnant and adult sheep of both genders will be used. Betamethasone is administered in a manner similar to that used for stimulating lung maturation in the unborn human, i.e., two doses of a mixture of the acetate and phosphate form, given 24 hours apart. The only difference is that we will adjust for maternal body weight and give 0.17 mg/kg with a maximum of 12 mg/day.