Notch Signaling in Joint Cartilage Maintenance and Arthritis

关节软骨维护和关节炎中的 Notch 信号传导

• 批准号: 8502631
• 负责人: Matthew J. Hilton
• 金额: $ 33.02万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-02 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8502631
• 关键词: Arthritis; Bone Development; Cartilage; Cartilage Matrix; Cell Differentiation process; Cells; Chondrocytes; Data; Degenerative polyarthritis; Dependence; Disease; Enzyme-Linked Immunosorbent Assay; Exhibits; Extracellular Matrix; Fibrosis; Gene Expression; Genes; Genetic; Genetic Models; Hereditary Disease; Histology; Human; Human Genetics; Immunohistochemistry; Impairment; In Situ Hybridization; In Vitro; Injury; Joints; Limb Development; Limb structure; Maintenance; Mediating; Mediator of activation protein; Meniscus structure of joint; Modeling; Molecular; Monitor; Mus; Mutant Strains Mice; Normal tissue morphology; Notch Signaling Pathway; Pathogenesis; Pathway interactions; Pharmaceutical Preparations; Phenotype; Population; Regulation; Reverse Transcriptase Polymerase Chain Reaction; Rosa; Sclerosis; Signal Transduction; Signaling Molecule; Stem cells; Tamoxifen; Tenascin; Testing; Time; Tissues; Western Blotting; articular cartilage; base; bone; density; genome wide association study; in vivo; joint injury; loss of function; lubricin; mouse model; mutant; notch protein; novel; overexpression; prevent; repaired; skeletal

DESCRIPTION (provided by applicant): Few signaling molecules or extracellular matrix (ECM) genes have been identified as critical regulators of joint cartilage maintenance in the pathogenesis of osteoarthritis (OA) in mice or humans. Recently, we have identified the Notch signaling pathway as a novel and critical regulator of skeletal progenitor cell differentiation during early limb development, as well as, an integral mediator of chondrocyte proliferation and maturation during endochondral bone development. Here we present additional breakthrough discoveries identifying the Notch signaling effector, RBPjk, as a novel and critical regulator of normal articular cartilage and joint maintenance. Specifically, we have discovered that loss of RBPjk in nearly all cells of the joints (Prx1Cre; RBPjkf/f) results in 1) fibrosis and degenerationof the articular cartilage with a significant loss in ECM components, 2) meniscus fibrosis and degeneration, 3) subchondral bone sclerosis, 4) osteophyte formation, and 5) a progressive loss of the Prg4 (lubricin) expressing superficial articular cartilage. Based on these novel findings, w hypothesize that chondrocyte-specific RBPjk-dependent Notch signaling is required for articular cartilage and joint maintenance via regulation of ECM-related molecules, which ultimately controls PRG4 (LUBRICIN) expression, localization, and function within the articular cartilage. To test this hypothesis we have developed three specific aims geared at uncovering the cellular and molecular mechanisms by which RBPjk- dependent Notch signaling maintains articular cartilage. We will generate several mouse genetic models and in vitro articular chondrocyte culture or explant models to test whether: A) cartilage-specific RBPjk-dependent Notch signaling controls articular cartilage maintenance, B) RBPjk-dependent Notch signaling is required to maintain normal PRG4 expression, localization, and function, C) RBPjk and PRG4 genetically and functionally interact during articular cartilage maintenance, D) PRG4 overexpression can rescue the OA phenotype of Prx1Cre; RBPjkf/f mutant mice, E) RBPjk haploinsufficiency accelerates OA progression following traumatic joint injury, and F) transient Notch activation can suppress OA progression following traumatic joint injury. Data generated by this proposal wil likely identify the RBPjk-dependent Notch pathway as a potential target for developing disease modifying osteoarthritis drugs (DMOADs).

描述(申请人提供)：在小鼠或人类骨关节炎(OA)的发病机制中，很少有信号分子或细胞外基质(ECM)基因被确定为关节软骨维持的关键调节因子。最近，我们发现Notch信号通路是肢体发育早期骨骼祖细胞分化的重要调节因子，也是软骨内骨发育过程中软骨细胞增殖和成熟的重要调节因子。在这里，我们提出了更多突破性的发现，确定Notch信号效应器RBPjk是正常关节软骨和关节维护的新的和关键的调节因子。具体地说，我们发现几乎所有关节细胞(Prx1Cre；RBPjkf/f)中RBPjk的丢失导致1)关节软骨纤维化和退变，其中ECM成分显著丢失，2)半月板纤维化和退变，3)软骨下骨硬化，4)骨赘形成，5)Prg4(润滑素)表达的浅表关节软骨进行性丧失。基于这些新的发现，我们假设软骨细胞特异性的RBPjk依赖的Notch信号是关节软骨和关节维持所必需的，通过调节ECM相关分子，最终控制PRG4(润滑素)在关节软骨中的表达、定位和功能。为了验证这一假设，我们开发了三个特定的目标，旨在揭示RBPjk依赖的Notch信号维持关节软骨的细胞和分子机制。我们将建立几个小鼠遗传模型和关节软骨细胞体外培养或移植模型，以测试：A)软骨特异的RBPjk依赖的Notch信号控制关节软骨的维持，B)RBPjk依赖的Notch信号需要维持正常的PRG4的表达、定位和功能，C)RBPjk和PRG4在关节软骨维持过程中在基因和功能上相互作用，D)PRG4的过表达可以挽救Prx1Cre的OA表型；RBPjkf/f突变小鼠，E)RBPjk单倍体缺陷加速创伤关节损伤后的OA进展，以及F)短暂的Notch激活可以抑制创伤关节损伤后的OA进展。这项提案产生的数据可能会将RBPjk依赖的Notch通路确定为开发修改疾病的骨关节炎药物(DMOADs)的潜在靶点。