Painless and TRPA1: TRP teamwork supports chemical nociception in Drosophila

无痛和 TRPA1：TRP 团队合作支持果蝇的化学伤害感受

• 批准号: 8409792
• 负责人: Vincent Cardin Panzano
• 金额: $ 1.04万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2013-05-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8409792
• 关键词: Animals; Asthma; Behavioral; Biological Assay; Brain; Calcium; Cells; Chemicals; Chronic; Cinnamon - dietary; Complement; Detection; Disease; Dominant-Negative Mutation; Drosophila genus; Elements; Eye; Genetic; Goals; Health; Heating; Histology; Human; Image; Immune Sera; Inflammatory; Insecta; Invertebrates; Ion Channel; Irritants; Measures; Mediating; Molecular; Molecular Genetics; Nature; Neurons; Nociception; Nociceptors; Onions; Pain; Painless; Physiological; Plants; Process; Publishing; RNA Interference; Research; Resolution; Role; Signal Transduction; Site; System; Taste Perception; Vertebrates; Work; cigarette smoking; design; disorder control; environmental chemical; experience; fly; insight; mutant; public health relevance; receptor; response; sensor; stimulus processing; ward

DESCRIPTION (provided by applicant): Anyone who has become teary-eyed cutting an onion, caught a whiff of wasabi, or tasted the spiciness of cinnamon, has experienced, first-hand, the painful potential of chemicals. Numerous plants and animals rely on damaging chemicals to ward off predators, and environmental, chemical irritants have been implicated in human inflammatory disorders, such as asthma. Studying how animals detect and avoid noxious chemicals - a process known as chemical nociception - may be useful for the design of effective pest repellents and for understanding how painful stimuli are processed in humans. In both vertebrates and invertebrates, the Transient Receptor Potential (TRP) ion channels cooperate during many aspects of nociception; however, the nature of this cooperation is poorly characterized. In Drosophila, two TRP channels - Painless and dTRPA1 - are required for flies to avoid reactive electrophiles, pungent chemicals active in wasabi, cinnamon, and cigarette smoke. dTRPA1 functions as a molecular sensor for these compounds, but Painless is unlikely to be a direct chemical sensor, making its role during this form of nociception unclear. The goal of this proposal is to characterize the function of Painless during dTRPA1- mediated chemical nociception. This work will contribute to our understanding of how TRP channels cooperate during nociception and will provide insights into the mechanisms of pain signaling at the molecular, cellular and circuit levels.

描述（由申请人提供）：任何已经泪流满面的人切开洋葱，捕获了芥末味或肉桂的辣味，这是第一手经历了化学物质的痛苦潜力。许多动植物依靠破坏性化学物质来抵御捕食者，环境，化学刺激物与人类炎症性疾病（例如哮喘）有关。研究动物如何检测和避免有害化学物质（一种称为化学伤害感受的过程）可能对设计有效的害虫驱虫剂的设计以及了解人类如何处理疼痛的刺激。在脊椎动物和无脊椎动物中，在伤害感受的许多方面，瞬时受体电位（TRP）离子通道都合作。但是，这种合作的性质的特征很差。在果蝇中，需要两个TRP通道（无痛和DTRPA1）才能避免反应性的电力，在芥末，肉桂和香烟烟雾中活跃的刺激性化学物质。 DTRPA1充当这些化合物的分子传感器，但无痛不太可能是直接的化学传感器，在这种形式的伤害感受中使其作用不清楚。该建议的目的是表征DTRPA1介导的化学伤害感受期间无痛的功能。这项工作将有助于我们理解TRP通道在伤害感受期间如何配合，并将提供有关分子，细胞和电路水平下疼痛信号传导机制的见解。