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Encoding and Interpreting Information at the Protein Level

在蛋白质水平上编码和解释信息

基本信息

批准号：
8549143
负责人：
ANDREAS MATOUSCHEK
金额：
$ 17.99万
依托单位：
NORTHWESTERN UNIVERSITY AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至 2015-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8549143
关键词：
Algorithms Amino Acid Sequence Amino Acids Atomic Force Microscopy Behavior Biochemical Bioinformatics Biological Assay Cells Chemicals Code Communities Elements Equation Equilibrium Family Fluorescence Gene Expression Lead Length Libraries Link Malignant Neoplasms Mechanics Methods Oncogene Proteins Peptide Signal Sequences Peptides Physiological Physiological Processes Process Production Property Proteasome Binding Protein Fragment Proteins Proteome Regulation Running Shapes Signal Transduction Site Software Tools Specific qualifier value Statistical Models Stretching Sum Tertiary Protein Structure Testing Translations Tumor Suppressor Proteins base cancer therapy computerized data processing inhibitor/antagonist multicatalytic endopeptidase complex novel novel therapeutic intervention optical traps physical model physical property polypeptide prevent protein structure research study single molecule tool transcription factor

项目摘要

Protein levels in the cell represent the sum of synthesis and degradation. The protein's (genetically encoded) amino acid sequence specifies not only the protein's structure, but also its lifetime through hidden degradation signals encoded in the sequence. Degradation rates balance gene expression rates and thus represent the second half of the equation that determines the active concentration of proteins. In the same way that aberrant gene expression is linked to cancer, so too is aberrant protein processing or degradation. The identity of a protein given by its amino is also dynamic and a combination of sequence elements encoding a degradation initiation signal and a degradation stop signal control the formation of protein fragments with activities distinct from those of the full length protein. This project will decode the sequence signals that shape the proteome and will determine the mechanical and chemical principles underlying these mechanisms.

细胞中的蛋白质水平代表合成和降解的总和。蛋白质的（基因编码的）氨基酸序列不仅指定了蛋白质的结构，还通过序列中编码的隐藏降解信号指定了其寿命。降解率平衡了基因表达率，因此代表了决定蛋白质活性浓度的方程的后半部分。就像异常的基因表达与癌症相关一样，异常的蛋白质加工或降解也与癌症相关。由其氨基给出的蛋白质的身份也是动态的，编码降解起始信号和降解停止信号的序列元件的组合控制具有与全长蛋白质不同的活性的蛋白质片段的形成。该项目将解码形成蛋白质组的序列信号，并将确定这些机制背后的机械和化学原理。