Animal Core

动物中心继续为所有PPG研究者提供基本支持， 为大鼠、小鼠和小牛的血流动力学测量提供服务。这包括平均值、收缩压和舒张压 肺和全身血管系统中的血压、心输出量和右/左心室 功能Core提供了在麻醉和清醒状态下测量这些参数的专业知识 使用各种系统的动物模型，包括Millar导管系统、超声心动图、压力填充 传感器和用于心输出量测量的染料稀释法。透过各类与 血流动力学测量的选择核心确保了个体实验设计可以 与涵盖所有研究者需求的适当数据收集系统相匹配。 除了血流动力学服务外，核心还维护并提供使用超高压和 低压舱设施从历史上看，这些腔室已被用于研究肺 高血压值得注意的是，该设施最近扩大到约400平方英尺。低压舱 通过容纳在单独区域中的单独真空泵减压。所有腔室均配备 自动装置在电源故障的情况下返回到环境条件。高压舱 通过过滤的压缩空气加压，并且通常用于将动物保持在海平面压力（760 mmHg）。 除了提供技术（血流动力学）和方法（心腔）支持外，核心还协助 保持近交系小鼠的繁殖集落和产生组织特异性敲除模型。 这包括维持近交系小鼠的繁殖集落和产生组织特异性敲除 模型核心将协助研究人员对这些动物进行育种、基因分型和饲养 根据需要的殖民地。此外，动物核心将参与靶向敲除的产生， 模型具体到该提案，该PPG项目的研究人员正在生成诱导平滑肌特异性 PTEN、PPARv和CREB敲除小鼠

The Animal Core continues to provide the fundamental support to all PPG investigators in terms of providing services for hemodynamic measurements in rats, mice and calves. This includes mean, systolic and diastolic blood pressures in the pulmonary and systemic vasculature, cardiac outputs and right/ left ventricular function. The Core provides the expertise to measure these parameters in the anesthetized and awake animal models with various systems including the Millar catheter system, echocardiography, pressure filled transducers, and dye dilution method for cardiac output measurements. By providing a wide range of options for hemodynamic measurements the core insures that the individual experimental design can be matched to the appropriate data collection system encompassing all of investigators needs. In addition to hemodynamic services the Core also maintains and provides assistance with use of the hyperand hypobaric chamber facility. Historically, these chambers have been used for the study of pulmonary hypertension. Notably, The facility has recently expanded in size to ~400 sq ft. The hypobaric chambers are depressurized by individual vacuum pumps housed in a separate area. All chambers are equipped with automatic devices to return to ambient conditions in case of power failure. The hyperbaric chambers are pressurized by filtered compressed air and typically used to maintain animals at sea level pressure (760 mmHg). Besides providing technical (hemodynamic) and methodological (chambers) support the Core also assists with maintenance of breeding colonies for inbred mice and generation of tissue-specific knockout models. This includes maintenance of breeding colonies for inbred mice and generation of tissue-specific knockout models. The Core will assist investigators with the breeding, genotyping, and maintenance of these animal colonies as needed. In addition, the Animal Core will be involved in the generation of targeted knockout models. Specific to this proposal, investigators of this PPG project are generating inducible smooth musclespecific PTEN, PPARv, and CREB knockout mice