Animal Core

动物核心继续为所有PPG调查人员提供基本支持，包括 为大鼠、小鼠和小牛的血液动力学测量提供服务。这包括平均、收缩和舒张期 肺和全身血管系统的血压、心输出量和右/左心室 功能。Core提供了在麻醉和清醒状态下测量这些参数的专业知识 具有各种系统的动物模型，包括Millar导管系统、超声心动图、压力充盈 用于心输出量测量的传感器和染料稀释法。通过提供广泛的 血流动力学测量的选项核心确保个人实验设计可以 与包含所有调查人员需求的适当数据收集系统相匹配。 除了血液动力学服务外，中心还维护和提供使用Hyperand 低压舱设施。从历史上看，这些小室曾被用来研究肺脏 高血压。值得注意的是，该设施最近扩大到约400平方英尺。低压舱是 通过安装在单独区域内的单个真空泵进行减压。所有房间都配备了 在断电的情况下，自动装置返回到环境条件。高压舱是 由过滤的压缩空气加压，通常用来维持动物在海平面上的压力(760 毫米汞柱)。 除了提供技术(血流动力学)和方法(心腔)支持外，核心还协助 通过维持近亲交配小鼠的繁育群体和产生组织特异性基因敲除模型。 这包括维持近交系小鼠的繁殖群体和产生组织特异性基因敲除 模特们。中心将协助调查人员对这些动物进行繁殖、基因分型和维护 根据需要建立殖民地。此外，动物核心将参与产生有针对性的击倒 模特们。具体到这个提议，这个PPG项目的调查人员正在产生诱人的流畅的肌肉特征 PTEN、PPARv和CREB基因敲除小鼠

The Animal Core continues to provide the fundamental support to all PPG investigators in terms of providing services for hemodynamic measurements in rats, mice and calves. This includes mean, systolic and diastolic blood pressures in the pulmonary and systemic vasculature, cardiac outputs and right/ left ventricular function. The Core provides the expertise to measure these parameters in the anesthetized and awake animal models with various systems including the Millar catheter system, echocardiography, pressure filled transducers, and dye dilution method for cardiac output measurements. By providing a wide range of options for hemodynamic measurements the core insures that the individual experimental design can be matched to the appropriate data collection system encompassing all of investigators needs. In addition to hemodynamic services the Core also maintains and provides assistance with use of the hyperand hypobaric chamber facility. Historically, these chambers have been used for the study of pulmonary hypertension. Notably, The facility has recently expanded in size to ~400 sq ft. The hypobaric chambers are depressurized by individual vacuum pumps housed in a separate area. All chambers are equipped with automatic devices to return to ambient conditions in case of power failure. The hyperbaric chambers are pressurized by filtered compressed air and typically used to maintain animals at sea level pressure (760 mmHg). Besides providing technical (hemodynamic) and methodological (chambers) support the Core also assists with maintenance of breeding colonies for inbred mice and generation of tissue-specific knockout models. This includes maintenance of breeding colonies for inbred mice and generation of tissue-specific knockout models. The Core will assist investigators with the breeding, genotyping, and maintenance of these animal colonies as needed. In addition, the Animal Core will be involved in the generation of targeted knockout models. Specific to this proposal, investigators of this PPG project are generating inducible smooth musclespecific PTEN, PPARv, and CREB knockout mice