Cytapheresis Of Volunteer Donors (MRI 2003-054)

在这个方案中，志愿者接受两个小时的细胞分离程序，从单一捐赠者那里收集大量循环中的白细胞成分(淋巴细胞或单核细胞)，供国家老龄研究所实验室研究人员使用。分离包作为匿名包提供给有资格的个人研究人员，用于淋巴细胞和单核细胞功能的基础研究以及衰老过程中的免疫功能研究。根据广泛修改的美国血库协会问卷，实验室筛查包括完整血细胞计数、尿液分析、肝和肾功能评估以及病毒学筛查(包括艾滋病毒和肝炎检测)，将外来感染或其他慢性医疗条件对实验室研究的影响降至最低。在过去一年中，既定的捐赠者人数保持不变，大约有70名参与者，共进行了221例手术。电池组被提供给个人研究人员用于研究目的。根据过去几年的趋势，一系列实验室对细胞的需求越来越大，预计明年将继续扩大参与者和该议定书中的程序。

In this protocol, volunteers undergo a two hour cytapheresis procedure for collection of large quantities of human circulating white blood cell components (lymphocytes or monocytes) from single donors for the use of National Institute on Aging laboratory investigators. Pheresis packs are supplied to individual qualified investigators as anonymous packs for use in basic studies of lymphocyte and monocyte function and studies of immune function in the aging process. Based on extensive modified American Bloodbank Association questionaires, laboratory screening including assessment of complete blood counts, urinalysis, liver and renal function as well as virologic screening including HIV and Hepatitis testing, the possibility of influence of extraneous infections or other chronic medical conditions on laboratory studies is minimized. In the course of the last year, the established donor pool has remained constant at approximately 70 participants with 221 procedures performed. Cell packs were supplied to individual investigators for research purposes. Based on trends over the last several years, there is an increasing need for cells by a range of laboratories with expectation for continued expansion of participants as well as procedures in this protocol in the next year.