Cytapheresis Of Volunteer Donors (MRI 2003-054)

志愿者捐献者的细胞分离术 (MRI 2003-054)

• 批准号: 9147452
• 负责人: Josephine Egan
• 金额: $ 4.23万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9147452
• 关键词: Aging-Related Process; American; Blood Component Removal; Cell Count; Cells; Cellular Structures; Chronic; Collection; Complete Blood Count; Cytapheresis; Genetic study; HIV; Hepatitis; Hour; Human; Individual; Infection; Laboratories; Laboratory Study; Leukocytes; Lymphocyte; Magnetic Resonance Imaging; Medical; Molecular Genetics; National Institute on Aging; Participant; Procedures; Process; Protocols documentation; Qualifying; Renal function; Research; Research Personnel; Sampling; Testing; Urinalysis; base; expectation; immune function; liver function; meetings; monocyte; screening; trend; volunteer

In this protocol, volunteers undergo a two hour cytapheresis procedure for collection of large quantities of human circulating white blood cell components (lymphocytes or monocytes) from single donors for the use of National Institute on Aging laboratory investigators. Pheresis packs are supplied to individual qualified investigators as anonymous packs for use in basic studies of lymphocyte and monocyte function and studies of immune function in the aging process. Based on extensive modified American Bloodbank Association questionaires, laboratory screening including assessment of complete blood counts, urinalysis, liver and renal function as well as virologic screening including HIV and Hepatitis testing, the possibility of influence of extraneous infections or other chronic medical conditions on laboratory studies is minimized. In the course of the last year, the established donor pool has remained constant at approximately 70 participants with 221 procedures performed. Cell packs were supplied to individual investigators for research purposes. Based on trends over the last several years, there is an increasing need for cells by a range of laboratories with expectation for continued expansion of participants as well as procedures in this protocol in the next year.

在该方案中，志愿者经历两小时的细胞分离术程序，以从单个供体收集大量人循环白色血细胞组分（淋巴细胞或单核细胞），供国家老龄化研究所实验室研究者使用。单采包以匿名包的形式提供给有资质的研究者，用于淋巴细胞和单核细胞功能的基础研究以及衰老过程中免疫功能的研究。根据广泛修改的美国血库协会调查问卷，实验室筛查（包括全血细胞计数评估、尿液分析、肝和肾功能）以及病毒学筛查（包括HIV和肝炎检测），将外源性感染或其他慢性疾病对实验室研究影响的可能性降至最低。在过去一年中，已建立的捐助者库保持不变，约有70名参与者，进行了221项程序。 细胞包提供给个体研究者用于研究目的。根据过去几年的趋势，一系列实验室对细胞的需求日益增加，预计明年将继续扩大参与者以及本方案中的程序。