SBIR TOPIC321:CHEMICALLY DEFINED GLYCAN LIBRARIES FOR REFERENCE STANDARDS AND GLY

SBIR 主题 321：参考标准品和 GLY 的化学定义聚糖库

• 批准号: 8758470
• 负责人: BAOLIN WU
• 金额: $ 29.95万
• 依托单位: CHEMILY, LLC
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-12 至 2014-06-11
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8758470
• 关键词: Anions; Biological Assay; Carbohydrates; Chickens; Chromatography; Complex; Custom; Development; Diagnostic; Disease; Egg Yolk; Ensure; Enzymes; Generations; Glycobiology; Glycoside Hydrolases; Goals; High Pressure Liquid Chromatography; Hybrids; Libraries; Monosaccharides; Oligosaccharides; Ovalbumin; Performance; Physiologic pulse; Polysaccharides; Process; Reference Standards; Research; Small Business Innovation Research Grant; Source; Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structure; Testing; Therapeutic; base; carbohydrate binding protein; chemical synthesis; detector; egg; glycosyltransferase; meetings

Access to a library of structurally defined N-glycans is an urgent scientific need for glyco-arrays for rapid analysis of carbohydrate binding proteins (CBPs), development of diagnostics and therapeutics for many diseases. However, only a small number of N-glycans are currently commercially available, which hampered researches in Glycobiology. This application will chemo-enzymatically prepare a library of 80 N-glycans (hybrid-, linear and complex-type) and commercialize them. The long-term goal is to produce thousands of natural N-glycans for carbohydrate-based highthroughput assays. Firstly, essential N-glycan precursors will be prepared in gram scales via either chemical synthesis or isolation from chicken eggs yolk and white. These N-glycans will then be enzymatically elongated or digested to yield 80 different N-glycans with defined structures (1 mg), among which only 12 N-glycans are currently available from commercial sources. The N-glycans contain 5 to 11 monosaccharide residues. This process involves several well characterized glycosyltransferases, glycoside hydrolases and other enzymes. All the N-glycans will be purified to 98%, and fully characterized by NMR and MS.

获得结构上确定的N-聚糖库是对用于快速分析N-聚糖的糖阵列的迫切科学需求。 碳水化合物结合蛋白（CBP），许多疾病的诊断和治疗的发展。但只有 目前市场上只有少量的N-聚糖，这阻碍了糖生物学的研究。 该应用将通过化学酶法制备80种N-聚糖（杂合型、线性型和复合型）的库，并 把它们商业化。长期目标是生产数千种天然N-聚糖，用于基于碳水化合物的高通量 分析。首先，必需的N-聚糖前体将通过化学合成或生物合成以克级制备。 从鸡蛋黄和白色中分离。然后将这些N-聚糖酶促伸长或消化以产生80 具有确定结构的不同N-聚糖（1 mg），其中目前只有12种N-聚糖可从 商业来源。N-聚糖含有5至11个单糖残基。这个过程涉及几个井 特征在于糖基转移酶、糖苷水解酶和其它酶。所有N-聚糖将被纯化至98%， 并通过NMR和MS充分表征。