Dermatological effects of gulf oil

海湾油的皮肤病学影响

• 批准号: 8422989
• 负责人: RANDLE Michael GALLUCCI
• 金额: $ 7.25万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-02-07 至 2014-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8422989
• 关键词: Acetone; Affect; Allergic; Area; Bacteria; CXCL1 gene; Cells; Contact Dermatitis; Data; Deposition; Dermal; Dermatitis; Dermis; Development; Disease; Enzyme-Linked Immunosorbent Assay; Epidermis; Event; Exposure to; Flow Cytometry; Formalin; Freezing; Goals; Guidelines; Health; Histology; Histopathology; Hour; Hypersensitivity; Imagery; Industry; Infiltration; Inflammation; Inflammatory; Inflammatory Response; Injury; Interleukin-1; Investigation; Irritant Dermatitis; Irritants; Light; Lung; Marines; Measures; Mexico; Microbe; Mus; Nature; Neurologic; Occupational; Oils; Pathology; Petroleum; Photography; Population; RNA; Recording of previous events; Reporting; Research; Ribosomal RNA; Risk Assessment; Sampling; Seawater; Sequence Analysis; Skin; Source; T-Lymphocyte; TNF gene; Temperature; Time; Weather; Work; Workers' Compensation; Workplace; allergic response; cytokine; man; microbial; skin disorder; skin irritant; skin lesion

DESCRIPTION (provided by applicant): The recent gulf BP oil spill is undoubtedly the worst man made oil spill in US history, dwarfing even the notorious Exxon Valdez spill in 1989. Oil spill clean up workers tend to have a myriad of health effects, such as skin, lung, and neurological problems. Inflammatory disorders of the skin are almost ubiquitous in the work place. Up to 50% of workers world-wide suffer from dermatitis at any time (Diepgen, 2007) and of reported occupational injury associated with workman's compensation in the US, contact dermatitis ranks second most prevalent overall, affecting workers in all industries (Beltrani, 2003). Dermatitis is divided into two main manifestations, that of irritant and allergic. The major difference between these pathologies is often described as whether the disease is of immunological origin (allergic) where T Cells are involved; or of non-immunological origin (irritant) where physical damage is thought to be the major initiating event. Since a very recent report found that oil samples from the Gulf Oil Spill were indeed a skin irritant, the focus of the first specific aim of this application will be to define the associated inflammatory response. Mice will be exposed to fresh and weathered oil or controls for seven days. Skin samples will be assessed for inflammatory cytokine expression, and inflammatory cell infiltration by histology and flow cytometry. The second specific aim will attempt to identify what components of spilled crude oil might be associated with dermatitis. First, skin of oil exposed mice will be extracted and petrochemicals will be determined by GC-MS analysis. There is also the possibility that microbial contamination of oil may lend itself to irritancy or allergy. Thus, marine bacteria populations will be assessed and identified from oil samples by rRNA analysis. The ultimate goal of this project is to identify the nature of oil spill dermatitis, allowing for rational risk assessment and exposure guidelines. Since little is known concerning oil spill health effects, these data will also aid in the further mechanistic investigations of this pathology by providing direction for future research.

描述（由申请人提供）：最近的海湾BP石油泄漏无疑是美国历史上最严重的人为石油泄漏，甚至使1989年臭名昭著的埃克森巴尔德斯泄漏事件相形见绌。石油泄漏清理工人往往有无数的健康影响，如皮肤，肺和神经问题。皮肤炎性疾病在工作场所几乎无处不在。全世界任何时候都有高达50%的工人患有皮炎（Diepgen，2007），在美国报告的与工人赔偿相关的职业伤害中，接触性皮炎总体上排名第二，影响所有行业的工人（Beltrani，2003）。 皮肤病分为两种主要表现，即刺激性和过敏性。这些病理学之间的主要区别通常被描述为疾病是否是免疫起源（过敏性），其中涉及T细胞;或非免疫起源（刺激性），其中物理损伤被认为是主要的起始事件。由于最近的一份报告发现，来自海湾石油泄漏的石油样品确实是一种皮肤刺激物，因此本申请的第一个具体目标的重点将是确定相关的炎症反应。将小鼠暴露于新鲜和风化油或对照品中7天。将通过组织学和流式细胞术评估皮肤样本的炎性细胞因子表达和炎性细胞浸润。第二个具体目标是试图确定泄漏原油的哪些成分可能与皮炎有关。首先，将提取油暴露小鼠的皮肤，并通过GC-MS分析确定石油化学物质。还有一种可能性是，油的微生物污染可能导致刺激或过敏。因此，将通过rRNA分析从石油样品中评估和鉴定海洋细菌种群。 该项目的最终目标是确定溢油皮炎的性质，以便进行合理的风险评估和制定接触准则。由于对溢油对健康的影响知之甚少，这些数据也将有助于通过为未来的研究提供方向来进一步研究这种病理学。

项目成果

Evaluation of skin irritation following weathered crude oil exposure in two mouse strains.

评估两种小鼠品系暴露于风化原油后的皮肤刺激。

• DOI: 10.1177/0748233720944354
• 发表时间: 2020
• 期刊: Toxicology and industrial health
• 影响因子: 1.9
• 作者: Kemp,JesseM;Luckett-Chastain,LerinR;Calhoun,KaitlinN;Frempah,Benjamin;Schartz,TaylerR;Harty,LindaE;Kemp,PhilipM;Gallucci,RandleM
• 通讯作者: Gallucci,RandleM