RNA Silencing in the Oral Cavity

口腔中的 RNA 沉默

• 批准号: 8314068
• 负责人: Andrew George Jakymiw
• 金额: $ 23.7万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8314068
• 关键词: Address; Animal Model; Apoptosis; Award; Biological; Biological Process; Cancer cell line; Cell physiology; Cells; Clinical; Clinical Trials; Communicable Diseases; Dentistry; Dependovirus; Disease; Drug Design; Effectiveness; Eye; Florida; Functional RNA; Gene Silencing; Gene Targeting; Genes; Goals; Head and Neck Squamous Cell Carcinoma; Human; In Vitro; Infectious Lung Disorder; Investigation; Laboratories; Lamin Type A; Link; Lung; Macular degeneration; Measures; Mediating; Mentors; Methods; Molecular; Mouth Diseases; Muscle; Neurodegenerative Disorders; Nuclear Envelope; Oncogenes; Oral Medicine; Oral cavity; Oral health; Oral mucous membrane structure; Patients; Pharmaceutical Preparations; Pharmacologic Substance; Pharmacotherapy; Phase; Principal Investigator; Process; Proteins; Publishing; RNA Interference; Recombinants; Reporter; Reporting; Research; Research Personnel; Role; Scientist; Skin; Small Interfering RNA; Staging; Subcellular structure; Techniques; Testing; Therapeutic; Tissues; Training; Transgenic Organisms; Translating; Universities; Use Effectiveness; Vagina; anticancer research; base; cancer cell; career; cell growth; college; effective therapy; enhanced green fluorescent protein; env Gene Products; gene function; gene therapy; improved; in vivo; inhibitor/antagonist; mRNA Decay; malignant mouth neoplasm; mouse model; new technology; novel therapeutic intervention; oral biology; oral tissue; small hairpin RNA; subcutaneous; tissue culture; tumor; tumor xenograft; viral gene delivery

DESCRIPTION (provided by applicant): Recently, our laboratory has demonstrated that cytoplasmic foci termed GW bodies (GWBs) for the GW182 protein they harbor are linked to an evolutionarily conserved mechanism involved in the post-transcriptional silencing of genes, referred to as RNA interference (RNAi). RNAi is a cellular process that is revolutionizing gene function studies and there is considerable excitement about using RNAi for therapy. Currently, our lab is focused on furthering the biological study of GW182 and GWB function, with the goal to ascertain the role of GWBs in the activation of the RNAi process and their contribution to regulating off-target effects. By furthering our understanding of the biological functions of GWBs, this should translate into improved drug designs for RNAi-based treatments. Despite the growing number of reports demonstrating the many exciting therapeutic aspects of RNAi, only a limited number of articles have been published regarding the application of RNAi in the treatment of oral diseases. It is therefore, the P.l.'s immediate goal to address this concern by bringing RNAi-based treatments to oral medicine during his transition from a mentored stage to becoming an independent scientific investigator. The long-term career objective is to establish himself as an expert in the field of oral health and RNAi-based therapies. A major advantage is that the oral mucosa is readily accessible to manipulation and may be especially suitable for RNAi-based treatments. It is therefore the principal investigator's plan to further his training in the field of oral biology at the University of Florida College of Dentistry, in particular in the field of oral cancer research. During the mentored phase of the award the principal investigator will characterize RNAi function in oral cancer cells, and optimize drug and viral gene delivery techniques in vitro and in vivo. Furthermore, the principal investigator will identify and target potential cancer-promoting gene targets in vitro using RNAi. During the independent phase of the award the principal investigator proposes to detemine the feasability of using RNAi-based drug and gene therapy approaches to treat a human tumor mouse model. The long-term research goal of this proposal is to exploit this novel technology and develop it into an effective therapy for oral cancer. By harnessing RNAi for oral medicine it will allow both scientists and clinicians to silence diseases of the oral cavity. Results from our proposed studies may have a broader impact applicable to treatment of other diseases using RNAi-based therapies.

描述（由申请人提供）：最近，我们的实验室已经证明，细胞质灶被称为GW小体（gwb），因为它们含有GW182蛋白，与基因转录后沉默的进化保守机制有关，称为RNA干扰（RNAi）。RNAi是一种革命性的基因功能研究的细胞过程，使用RNAi进行治疗非常令人兴奋。目前，我们实验室正致力于进一步开展GW182和GWB功能的生物学研究，以确定GWB在激活RNAi过程中的作用及其在调控脱靶效应中的作用。通过进一步了解gwb的生物学功能，这应该转化为改进基于rnai治疗的药物设计。尽管越来越多的报道展示了RNAi的许多令人兴奋的治疗方面，但关于RNAi在口腔疾病治疗中的应用的文章数量有限。因此，p.l.。在他从导师阶段过渡到成为一名独立的科学研究者期间，他的直接目标是通过将基于rnai的治疗方法引入口腔医学来解决这一问题。他的长期职业目标是成为口腔健康和基于rnai的治疗领域的专家。一个主要的优点是口腔黏膜易于操作，可能特别适合基于rnai的治疗。因此，首席研究员计划在佛罗里达大学牙科学院继续他在口腔生物学领域的培训，特别是在口腔癌研究领域。在该奖项的指导阶段，首席研究员将表征RNAi在口腔癌细胞中的功能，并优化体外和体内药物和病毒基因传递技术。此外，首席研究员将在体外使用RNAi识别和靶向潜在的促癌基因靶点。在该奖项的独立阶段，首席研究员建议确定使用基于rnai的药物和基因治疗方法治疗人类肿瘤小鼠模型的可行性。本课题的长期研究目标是开发这项新技术，并将其发展成为口腔癌的有效治疗方法。通过将RNAi用于口腔医学，它将使科学家和临床医生能够使口腔疾病沉默。我们提出的研究结果可能对使用基于rnai的疗法治疗其他疾病具有更广泛的影响。