权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular Basis of Tooth Eruption

牙齿萌出的分子基础

基本信息

批准号：
8277789
负责人：
Shaomian Yao
金额：
$ 34.94万
依托单位：
LOUISIANA STATE UNIV A&M COL BATON ROUGE
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-07-01 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8277789
关键词：
Affect Alveolar Alveolar Bone Loss Bone Growth Bone Resorption Cell fusion Cells Coculture Techniques Coupled Dendritic Cells Dental Sac Disease Down-Regulation Electroporation Event Excision Gene Expression Genes Goals Impacted Tooth Incubated Injection of therapeutic agent Integral Membrane Protein Macrophage Colony-Stimulating Factor Messenger RNA Methods Mind Molecular Osteoblasts Osteoclasts Osteogenesis Pathway interactions Periodontal Ligament Periodontitis Population Progeria Proteins RNA Interference Regulation Reverse Transcriptase Polymerase Chain Reaction Role Scanning Electron Microscopy Small Interfering RNA Stem cells Syndrome TNFSF11 gene Technology Testing Time Tooth eruption Up-Regulation Vascular Endothelial Growth Factors alveolar bone base bone morphogenetic protein 2 frizzled related protein-1 in vivo laser capture microdissection osteoclastogenesis osteogenic precursor cell prevent stem cell differentiation

项目摘要

Project Summary. Tooth eruption requires alveolar bone resorption and bone formation. We have shown that the osteoclastogenesis needed for resorption of alveolar bone in the coronal region of the bony crypt is regulated by expression of osteoclastogenesis genes in the dental follicle (DF). Similarly, we hypothesize that osteo-inductive genes expressed in the DF, especially those genes upregulated in the basal one-half of the DF, promote tooth eruption by regulating the osteogenesis needed for bone formation that occurs at the base of the alveolar bony crypt. To test this hypothesis, gene microarray studies will be done to determine which osteo-inductive genes are upregulated in the DF at the times of maximal bone growth at the base of the socket. Next, because surgical removal of the basal one-half of the DF prevents tooth eruption and alveolar bone formation, laser capture microdissection coupled with real-time RT-PCR studies will determine which of these osteo-inductive genes are expressed more in the basal portion than in the coronal portion of the DF. Each gene that is upregulated more in the basal portion will then be silenced in vivo using electroporation and RNAi technology specific for the target mRNA of each gene to determine the effect of each gene on eruption times. Regarding osteoclastogenesis, secreted frizzled-related protein-1 (SFRP-1), a molecule that inhibits osteoclastogenesis, is expressed in the DF and it is hypothesized that its gene expression is downregulated by eruption molecules such that osteoclastogenesis can occur. Thus, DF cells will be incubated with molecules that promote osteoclastogenesis for eruption to determine which of them down-regulate SFRP-1 expression. Using osteoclast precursor cells, we also will determine if SFRP-1 inhibits osteoclastogenesis by down- regulating a cell fusion molecule, DC-STAMP. Finally, we hypothesize that stem cells present in the DF might contribute to the osteoclast precursors and osteoblasts needed for eruption. To test this, stem cells isolated from the DF will be incubated with osteoclast-inducing molecules or with osteo-inductive molecules to determine if the stem cells can form osteoclasts or osteoblasts. Relevance. Understanding the molecular regulation of the osteogenesis and osteoclastogenesis needed for eruption may explain why impacted teeth (e.g., 3rd molars) do not erupt, as well as explain the causes of various eruption disorders such as seen in Hutchinson-Gilford Progeria syndrome. To correct eruption disorders, a molecular approach to induce eruption is a long-term goal. Finally, because the periodontal ligament (PDL) is derived from the DF, the molecular regulation of osteoclastogenesis by the DF may suggest a similar role for the PDL in regulating alveolar bone loss seen in periodontitis.

项目总结。萌牙需要牙槽骨吸收和骨形成。我们已经证明了

项目成果

期刊论文数量（60）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Chronological gene expression of parathyroid hormone-related protein (PTHrP) in the stellate reticulum of the rat: implications for tooth eruption.

DOI：
10.1016/j.archoralbio.2006.10.008
发表时间：
2007-03
期刊：
Archives of oral biology
影响因子：
3
作者：
S. Yao;F. Pan;G. Wise
通讯作者：
S. Yao;F. Pan;G. Wise

Evaluation of bone regeneration potential of dental follicle stem cells for treatment of craniofacial defects.

DOI：
10.1016/j.jcyt.2015.07.013
发表时间：
2015-11
期刊：
Cytotherapy
影响因子：
4.5
作者：
Rezai-Rad M;Bova JF;Orooji M;Pepping J;Qureshi A;Del Piero F;Hayes D;Yao S
通讯作者：
Yao S

The role of dentin matrix protein 1 (DMP1) in regulation of osteogenic differentiation of rat dental follicle stem cells (DFSCs).

DOI：
10.1016/j.archoralbio.2014.12.013
发表时间：
2015-04
期刊：
ARCHIVES OF ORAL BIOLOGY
影响因子：
3
作者：
Rad, Maryam Rezai;Liu, Dawen;He, Hongzhi;Brooks, Hunter;Xiao, Mei;Wise, Gary E.;Yao, Shaomian
通讯作者：
Yao, Shaomian

Protein kinase A expression and its possible roles in regulating tooth eruption genes in the dental follicle.

蛋白激酶 A 的表达及其在调节牙囊中牙齿萌出基因中的可能作用。