Nitric oxide and mitochondrial biogenesis in sepsis

脓毒症中的一氧化氮和线粒体生物发生

基本信息

  • 批准号:
    8534342
  • 负责人:
  • 金额:
    $ 31.4万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2012
  • 资助国家:
    美国
  • 起止时间:
    2012-08-23 至 2014-01-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): This revised competitive renewal application seeks to understand how nitric oxide (NO) production in bacterial sepsis causes both mitochondrial damage and regulates mitochondrial quality control, which protects against organ failure. In the previous grant cycle, we discovered that toll-like receptor (TLR) activation of NF-kB- dependent NO synthase (iNOS/NOS2) is involved in the regulation of hepatic mitochondrial biogenesis through the major co-activator, PGC-11 and the adenosine monophosphate (AMP)-activated protein kinase (AMPK). Our preliminary data in mice with sepsis indicate that: NOS2 induction leads to AMPK activation, AMPK helps to activate mitochondrial biogenesis, but not always after ATP depletion, and NOS2 deficiency diminishes AMPK up-regulation and mitochondrial biogenesis and increases apoptosis and inflammation in response to TLR activation. Thus, AMPK appears to activate mitochondrial biogenesis while opposing apoptosis and inflammation in sepsis. In contrast, too much NO is independently associated with mitochondrial damage and loss of NO signal specificity by chemical attack of NO species (NOx) on proteins and nucleic acids. We hypothesize that NOS2, acting in part through AMPK, is required for apposite regulation of the transcriptional program of mitochondrial biogenesis before the failure of ATP production in order to maintain mitochondrial quality control and prevent cell death in sepsis. A test of this hypothesis requires definitions of NO-dependent transcriptional control mechanisms, the role of AMPK, and the chemical biology of mitochondrial NO in relevant model systems. Our approach will focus on the liver as a sentinel organ and on one genetic factor- NOS2- as a quantitative influence on mitochondrial turnover and cell survival. We plan to test these Specific Aims: Aim 1: To determine the role of NOS2 on hepatic mitochondrial biogenesis and cell survival during severe sepsis through quantitative NOS2 gene titration studies. 1A. Define the relationships between NOx-mediated mtDNA and protein damage, respiratory capacity, high-energy metabolite levels, and sepsis-induced hepatic cell death using NOS2 titration. 1B. Assess the importance of NOS2 expression on the regulation of mitochondrial biogenesis in sepsis through CREB and/or NRF-1 induction and regulation of NRF-21 (GABPA) and/or PGC-11 expression. Aim 2: To understand hepatic AMPK activation in sepsis in relation to NOS2, the transcriptional program of mitochondrial biogenesis, and prevention of apoptosis. 2A. Determine if NO-dependent AMPK activation promotes mitochondrial biogenesis in sepsis through CREB and/or prevents apoptosis by inhibitory phosphorylation of pro-apoptotic proteins, Bad and BNIP3. 2B. Determine if pharmacological activation of AMPK in sepsis can promote mitochondrial biogenesis and/or inhibit apoptosis independently of NOS2. These studies will provide new insights into NOx-induced mitochondrial damage in sepsis in the context of physiological mechanisms by which NOS2 regulates mitochondrial biogenesis and the extent to which it is orchestrated by AMPK. By implication, NO regulation of AMPK would play a critical salvage role in MODS, and this knowledge would allow rational new pharmacological approaches to support mitochondrial function while minimizing collateral damage by NOx.
描述(申请人提供):这份修订后的竞争性续签申请旨在了解细菌败血症中一氧化氮(NO)的产生如何导致线粒体损伤并调节线粒体质量控制,从而保护器官免受衰竭。在之前的赠款周期中,我们发现,Toll样受体(Toll-like Receptor,TLR)激活的核因子-kB依赖的一氧化氮合酶(iNOS/NOS2)通过主要的共激活因子PGC-11和腺苷一磷酸(AMP)激活的蛋白激酶(AMPK)参与了肝线粒体生物发生的调节。我们在脓毒症小鼠中的初步数据表明:NOS2诱导导致AMPK激活,AMPK有助于激活线粒体生物合成,但在ATP耗尽后并不总是如此,NOS2缺乏减少了AMPK上调和线粒体生物生成,并增加了对TLR激活的细胞凋亡和炎症反应。因此,在脓毒症中,AMPK似乎激活了线粒体的生物发生,同时对抗了细胞凋亡和炎症。相反,过多的NO与线粒体损伤和NO物种对蛋白质和核酸的化学攻击(NOx)导致的NO信号特异性丧失独立相关。我们推测,在ATP产生失败之前,部分通过AMPK作用的NOS2是适当调节线粒体生物发生的转录程序所必需的,以维持线粒体的质量控制,防止脓毒症中的细胞死亡。对这一假设的检验需要定义依赖于NO的转录调控机制、AMPK的作用以及相关模型系统中线粒体NO的化学生物学。我们的方法将重点放在肝脏作为前哨器官和一个遗传因素-NOS2-作为对线粒体周转和细胞生存的定量影响上。我们计划测试这些特定的目标:目的1:通过定量的NOS2基因滴定研究,确定NOS2在严重脓毒症时对肝脏线粒体生物发生和细胞存活的作用。1A.使用NOS2滴定,确定NOx介导的线粒体DNA与蛋白质损伤、呼吸能力、高能代谢物水平以及败血症诱导的肝细胞死亡之间的关系。1B.通过CREB和/或NRF-1的诱导以及对NRF-21(GABPA)和/或PGC-11表达的调节,评估NOS2表达在脓毒症线粒体生物发生调控中的重要性。目的:了解脓毒症大鼠肝脏AMPK活性与线粒体生物发生转录程序NOS2的关系及对细胞凋亡的抑制作用。2a.确定NO依赖的AMPK激活是否通过CREB促进脓毒症中线粒体的生物生成,和/或通过抑制促凋亡蛋白Bad和BNIP3的磷酸化来阻止细胞凋亡。2B。确定AMPK在脓毒症中的药理激活是否可以促进线粒体的生物发生和/或抑制细胞凋亡,而不依赖于NOS2。这些研究将在NOx调节线粒体生物发生的生理机制以及AMPK在多大程度上调控线粒体生物发生的背景下,为了解NOx诱导的脓毒症线粒体损伤提供新的见解。这意味着,AMPK的任何调控都不会在MODS中发挥关键的挽救作用,这一知识将允许合理的新药理学方法来支持线粒体功能,同时将NOx的附带损害降至最低。

项目成果

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CLAUDE A PIANTADOSI其他文献

CLAUDE A PIANTADOSI的其他文献

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{{ truncateString('CLAUDE A PIANTADOSI', 18)}}的其他基金

Respiration in Sepsis
脓毒症时的呼吸
  • 批准号:
    8436690
  • 财政年份:
    2013
  • 资助金额:
    $ 31.4万
  • 项目类别:
Respiration in Sepsis
脓毒症时的呼吸
  • 批准号:
    8666533
  • 财政年份:
    2013
  • 资助金额:
    $ 31.4万
  • 项目类别:
Respiration in Sepsis
脓毒症时的呼吸
  • 批准号:
    8971980
  • 财政年份:
    2013
  • 资助金额:
    $ 31.4万
  • 项目类别:
Redox Regulation of Lung Mitochondrial Biogenesis in Sepsis/Pneumonia
脓毒症/肺炎中肺线粒体生物发生的氧化还原调节
  • 批准号:
    8370970
  • 财政年份:
    2012
  • 资助金额:
    $ 31.4万
  • 项目类别:
Redox Regulation of Lung Mitochondrial Biogenesis in Sepsis/Pneumonia
脓毒症/肺炎中肺线粒体生物发生的氧化还原调节
  • 批准号:
    8462898
  • 财政年份:
    2012
  • 资助金额:
    $ 31.4万
  • 项目类别:
Redox Regulation of Lung Mitochondrial Biogenesis in Sepsis/Pneumonia
脓毒症/肺炎中肺线粒体生物发生的氧化还原调节
  • 批准号:
    8675191
  • 财政年份:
    2012
  • 资助金额:
    $ 31.4万
  • 项目类别:
Carbon Monoxide and Mitochondrial Quality Control in Sepsis-induced Lung Injury
脓毒症引起的肺损伤中的一氧化碳和线粒体质量控制
  • 批准号:
    8225578
  • 财政年份:
    2011
  • 资助金额:
    $ 31.4万
  • 项目类别:
Mitochondrial biogenesis in sepsis-induced organ dysfunction
脓毒症引起的器官功能障碍中的线粒体生物发生
  • 批准号:
    8217199
  • 财政年份:
    2009
  • 资助金额:
    $ 31.4万
  • 项目类别:
Mitochondrial biogenesis in sepsis-induced organ dysfunction
脓毒症引起的器官功能障碍中的线粒体生物发生
  • 批准号:
    8021807
  • 财政年份:
    2009
  • 资助金额:
    $ 31.4万
  • 项目类别:
Mitochondrial biogenesis in sepsis-induced organ dysfunction
脓毒症引起的器官功能障碍中的线粒体生物发生
  • 批准号:
    7782730
  • 财政年份:
    2009
  • 资助金额:
    $ 31.4万
  • 项目类别:

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