Microscopy

项目总结（见说明）： 显微镜共享资源（MSR）是一个位于中心的资源，拥有尖端仪器和训练有素的专家，为OSUCCC科学家提供共聚焦，光学，扫描电子和透射电子显微镜的出色支持，MSR有两台电子显微镜和四台共聚焦镜，所有这些都是自2005年以来通过联邦赠款和出色的机构支持购买的。这些仪器包括两个单光子Olympus FVI 000共聚焦显微镜，每个显微镜具有四个激光器和高N. A。专门用于固定细胞和组织的物镜、FEI Tecnai BioTwin透射电子显微镜和Olympus FVI 000多光子共聚焦仪器，配备MaiTai DeepSee激光器，可深入探测活体动物和固定组织中的肿瘤。Richard Burry博士是一位成熟且资金充足的科学家，拥有30多年的显微镜专业知识，他沿着经验丰富且训练有素的工作人员，为OSUCCC研究人员提供实验设计和图像分析方面的重要咨询。通过组织良好的培训课程和工作人员提供的个人培训，提高了MSR的使用率和生产率。MSR被广泛用于OSUCCC的科学项目，为超过30个OSUCCC成员实验室提供服务，为高质量的癌症相关出版物和赠款生成图像。MSR位于生物医学研究塔（BRT）的二楼，靠近OSUCCC成员的实验室。基于MSR的扩展能力和OSUCCC成员的赠款申请数量的增加，OSUCCC的使用量预计将在未来五年内根据战略招聘目标大幅增加， 癌症研究对高端显微镜的需求扩大。MSR通过利用与OSU学院，OSU研究办公室的广泛合作伙伴关系，以及俄亥俄州和OSUCCC的赠款，获得了杰出的机构资源的支持。MSR是一种新的OSUCCC共享资源，以前作为OSU核心设施拥有强大的用户基础，因此实现了NIH的目标，即巩固核心设施，以实现NIH资助的研究人员的最大效率和利用率。总的来说，MSR是一个关键的 OSUCCC研究人员的共享资源，旨在识别正常组织和肿瘤中的特定细胞和蛋白质，以增强我们对癌症基本过程的理解，从而制定治疗策略。

PROJECT SUMMARY (See instructions): The Microscopy Shared Resource (MSR) is a centrally-located resource with cutting-edge instruments and highly trained experts to provide outstanding support for OSUCCC scientists for confocal, light, scanning electron and transmission electron microscopy, the MSR has two electron microscopes and four confocals all purchased since 2005 through federal grants and outstanding institutional support. These instruments include two single photon Olympus FVI 000 confocal microscopes each with four lasers and high N.A. objectives specifically for fixed cells and tissue, an FEI Tecnai BioTwin transmission electron microscope, and an Olympus FVI 000 multiphoton confocal instrument with a MaiTai DeepSee laser to probe deep into tumors in both live animals and fixed tissue. The MSR is led by Dr. Richard Burry, an established and well funded scientist with over 30 years of extensive expertise in microscopy, who along with an experienced and highly-trained staff, provides OSUCCC investigators vital consultation in experimental design and image analysis. Usage and productivity from the MSR is enhanced by well-organized training courses and individual training offered by staff members. The MSR is extensively used across OSUCCC scientific programs providing service to >30 OSUCCC member labs to generate the images for high quality cancer relevant publications and grants. The MSR is centrally located on the second floor of the Biomedical Research Tower (BRT) close to the labs of the OSUCCC members. Based on the expanding capabilities of the MSR and increases in number of grant applications from OSUCCC members, the OSUCCC usage is estimated to increase dramatically over the next five years based on strategic recruitment goals and expanded demand for high-end microscopy in cancer research. The MSR is supported by outstanding institutional resources by leveraging extensive partnerships with OSU Colleges, the OSU Office of Research, grants from the State of Ohio and the OSUCCC. The MSR is a new OSUCCC shared resource, previously with a strong user base as an OSU core facility, and thus fulfills NIH goals to consolidate core facilities for maximal efficiency and utilization by NIH funded investigators. Collectively the MSR is a critical shared resource for OSUCCC investigators seeking to identify specific cells and proteins in normal tissue and in tumors to enhance our understanding of fundamental processes of cancer in developing therapeutic strategies.