Determining the genetic and developmental basis of epidermal appendage evolution

确定表皮附属物进化的遗传和发育基础

• 批准号: 8524600
• 负责人: Eric Domyan
• 金额: $ 4.92万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8524600
• 关键词: Adult; Ankle; Area; Back; Biochemical; Biology; Birds; Body Size; Breeding; Candidate Disease Gene; Cell Maintenance; Cells; Characteristics; Color; Columbidae; Communication; Coupled; Data; Defect; Development; Disease; Embryo; Embryonic Development; Eph Family Receptors; Epithelial; Event; Evolution; Feathers; Gene Expression; Genes; Genetic; Genetic Crosses; Genetic Polymorphism; Genomics; Goals; Growth; Hair; Head; Homeostasis; Human; In Vitro; Individual; Lead; Location; Locomotion; Malignant Neoplasms; Mammals; Maps; Mesenchymal; Microsurgery; Modeling; Molecular; Morphology; Nature; Organ; Organism; Pattern Formation; Physiologic Thermoregulation; Population; Prevention; Price; Process; Public Health; Quantitative Trait Loci; Receptor Signaling; Research; Rock Pigeons; Role; Sebaceous Glands; Signal Transduction; Skin; Stem cells; Structure; Study models; Techniques; Testing; Tissue Grafts; Tissue Recombination; Tissues; Variant; Visual; Wing; Work; Wound Healing; appendage; base; craniofacial; developmental genetics; fitness; foot; gene function; genetic manipulation; insight; interest; molecular phenotype; novel; public health relevance; research study; stem cell biology; stem cell population; tissue regeneration; trait

DESCRIPTION (provided by applicant): The development of epidermal appendages, such as hair in mammals and feathers and scales in birds, provides a particularly tractable model for studying medically-relevant processes such as fate determination, epithelial-mesenchymal interactions, stem cell maintenance, and lineage commitment. In adult organisms, disregulation of appendage homeostasis can lead to diseases such as cancer, highlighting the importance of understanding how development is controlled. In addition, these appendages have important fitness functions such as thermoregulation, protection from physical damage, and in the case of birds, locomotion and visual communication. Although hair and feathers are defining features of mammals and birds, the placement and morphology of these structures varies widely from organism to organism. While research has identified some of the genetic mechanisms regulating formation of these appendages, little is known about the genetic and developmental mechanisms that allow their evolution. Selective breeding has produced tremendous phenotypic diversity among closely-related domestic pigeon breeds. This diversity, coupled with the experimental accessibility of their embryos, makes pigeons an powerful model for integrating evolutionary and developmental genetics. One interesting trait present in multiple breeds is the formation of head crests, whereby feathers on the back of the head have a reversed polarity, and grow forwards instead of backwards. In addition, while many pigeon breeds have scales on their feet, in a number of breeds, scales have been replaced by feathers. The proposed research will utilize domestic pigeon breeds that possess these traits to understand the genetic and developmental mechanisms that regulate epidermal appendage polarity and fate, with three aims. First, a strong candidate gene associated with crest formation will be analyzed through genetic and biochemical studies to understand its role in determining appendage polarity. Second, a candidate genomic region associated with the determination of feather vs. scale fate will be narrowed through a combination of population genomics analyses and quantitative trait locus mapping. Third, tissue recombination experiments will be used to identify how signaling interactions have changed during the evolution of foot feathering, and candidate genes identified in Aim 2 will be tested through genetic manipulation of pigeon embryos to study their role in determining epidermal organ fate. This work will integrate multiple levels of research to generate insight into how epidermal organ fate and polarity are determined, and how these traits are altered during evolution. This work also will pioneer development of the domestic pigeon as a powerful new model of vertebrate evolution and development, and will generate insights into the basis of epidermal development and disease in humans.

描述（由申请人提供）：表皮附属物的发育，如哺乳动物的毛发和鸟类的羽毛和鳞片，为研究医学相关过程提供了一个特别容易处理的模型，如命运决定、上皮-间充质相互作用、干细胞维持和谱系定型。在成年生物体中，附件稳态失调可能导致癌症等疾病，这突出了了解发育是如何控制的重要性。此外，这些附肢具有重要的健身功能，如体温调节，保护免受物理伤害，以及鸟类的运动和视觉交流。虽然毛发和羽毛是哺乳动物和鸟类的特征，但这些结构的位置和形态在不同的生物体中有很大的差异。虽然研究已经确定了一些调节这些附属物形成的遗传机制，但对允许它们进化的遗传和发育机制知之甚少。选择性育种在近缘家鸽品种中产生了巨大的表型多样性。这种多样性，加上胚胎的实验可及性，使鸽子成为整合进化和发育遗传学的强大模型。一个有趣的特点是在多个品种的形成头冠，即羽毛的头部后面有一个相反的极性，并向前而不是向后生长。此外，虽然许多鸽子品种的脚上有鳞片，但在一些品种中，鳞片已被羽毛取代。拟议的研究将利用具有这些特征的家鸽品种来了解调节表皮附属物极性和命运的遗传和发育机制，有三个目标。首先，将通过遗传和生化研究分析与嵴形成相关的强候选基因，以了解其在确定附属物极性中的作用。第二，通过群体基因组学分析和数量性状基因座定位的结合，缩小与羽毛与鳞片命运决定相关的候选基因组区域。第三，组织重组实验将被用来确定如何信号相互作用已经改变了脚羽的进化过程中，目标2中确定的候选基因将通过鸽子胚胎的遗传操作进行测试，以研究它们在决定表皮器官命运的作用。这项工作将整合多个层次的研究，以深入了解表皮器官的命运和极性是如何决定的，以及这些特征在进化过程中是如何改变的。这项工作还将开创家鸽作为脊椎动物进化和发育的强大新模型的发展，并将深入了解人类表皮发育和疾病的基础。