The fates of duplicate genes at the subcellular level

描述(由申请者提供)：这是一个F32，为期三年的博士后奖学金。建议的研究是在进化遗传学和细胞生物学领域。本研究提出的方法包括生物信息学、基因组学、选择标记分析、分子遗传学、显微镜和生物化学。我建议利用一种新的方法在亚细胞水平上理解重复基因的命运。我计划将重复基因的基因组分析的力量与荧光标记的对虾的亚细胞共定位研究结合起来。我将使用Paralog融合信号中的重叠量来衡量物种内和物种间Paralog之间的功能重叠。使用涉及膜转运的基因家族，其蛋白质决定因素以空间特定的方式发挥作用，将使我能够将功能与亚细胞定位联系起来。草履虫物种复合体中相对较新的全基因组复制和物种辐射，以及草履虫基因组之间高度的同步性，为进行这一分析提供了一个强大的基因组系统。这项工作将进一步加深对重复基因进化的原因和功能后果的理解，这对进化遗传学以及我们对基础真核细胞生物学的理解都具有重要意义。

DESCRIPTION (provided by applicant): This grant proposal is for an F32, three-year postdoctoral fellowship. The research proposed is in the fields of evolutionary genetics and cell biology. The methods proposed for this research include bioinformatics, genomics, and analysis of signatures of selection, molecular genetics, microscopy and biochemistry. I propose utilizing a novel approach to understanding the fates of duplicate genes at the subcellular level. I plan to combine the power of genomic analysis of duplicate genes with subcellular colocalization studies of fluorescently tagged paralogs. I will use the amount of overlap in signal of paralog fusions to gauge the overlap in function between both intra- and interspecies paralogs. The use of gene families involved in membrane trafficking, whose protein determinants act in a spatially specific manner, will allow me to correlate function with subcellular localization. The relatively recent whole genome duplications and species radiation within the Paramecium aurelia species complex, combined with a high degree of synteny between Paramecium genomes, offer a powerful genomic system in which to conduct this analysis. This work will further the understanding of both the evolutionary causes and the functional consequences of the evolution of duplicate genes, which has implications for evolutionary genetics as well our understanding of basic eukaryotic cell biology.