FY 2013 Contract HHSN271201300012C, SBIR-1, Topic 003, Automated Instrument for C

2013 财年合同 HHSN271201300012C，SBIR-1，主题 003，C 自动化仪器

• 批准号: 8756438
• 负责人: JIMMY LU
• 金额: $ 15.85万
• 依托单位: CODEX BIOSOLUTIONS, INC.
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-10 至 2014-03-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8756438
• 关键词: Applications Grants; Biological Assay; Cells; Chemicals; Contracts; Cytoplasm; Dyes; Engineering; Fluorescence; Matrix Metalloproteinases; Measures; Membrane Potentials; Mitochondria; Parents; Phase; Property; Small Business Innovation Research Grant; Solubility; Time; Toxic effect; Work; base; cost; hazard; high throughput screening; instrument; mitochondrial membrane; monomer; novel; water solubility

In this SBIR phase I grant application, Codex BioSolutions proposes to construct a cell-based high throughput assay that measures chemical toxicity using a novel dye, m-MPI. This dye was engineered to overcome many of the drawbacks of current dyes used to measure mitochondrial membrane potential. The parent dye JC-1 has been used for this purpose previously. In the mitochondria, where it accumulates as aggregates, it fluoresces red, whereas in the cytoplasm where it is a monomer, it fluoresces green. However, JC-1 is difficult to use because it has very low water solubility which makes using its differential fluorescent properties difficult. Our dye, m-MPI has increased solubility allowing easy quantitative assessment of MMP status simply by calculating the ratio of red/green fluorescence. m-MPI can also be used with cells that do not work with JC-1 (such as primary cells) due at least in part to its increased sensitivity. We believe our assay will more accurately assess hazards in a shorter time and with less cost.

在这项SBIR第一阶段授权申请中，Codex BioSolutions建议构建一种基于细胞的高通量分析，使用一种新型染料m-MPI来测量化学毒性。这种染料的设计是为了克服许多 目前用于测量线粒体膜电位的染料的缺点。母体染料JC-1以前曾用于此目的。在线粒体中，它以聚集的形式聚集，发出红色的荧光，而在细胞质中，它是单体，发出绿色的荧光。然而，JC-1很难使用，因为它的水溶性很低，这使得使用其差示荧光性能变得困难。我们的染料m-MPI具有更高的溶解度，只需计算红色/绿色荧光的比率，即可轻松定量评估基质金属蛋白酶的状态。M-MPI也可以用于不能与JC-1一起工作的细胞(如原代细胞)，至少部分原因是它的敏感性增加。我们相信，我们的检测将在更短的时间内以更低的成本更准确地评估危险。