Training Program on HIV Diversity and Drug Resistance-Enhancing Research Capacity

项目总结/摘要 喀麦隆广泛的HIV-1基因多样性和抗药性变异的出现， 使病毒遗传多样性复杂化并对诊断和预防工作造成潜在问题， 要求监测HIV-1多样性的演变，并检查新的HIV-1变异体， 不同HIV-1检测试剂盒的检测灵敏度。这些问题继续突出表明，需要进一步 在喀麦隆进行培训和研究。这项名为“艾滋病毒多样性和毒品问题培训方案”的培训方案 阻力：提升科研能力”是以长期建设和强化科研能力为设计目标 在喀麦隆雅温得的医学诊断中心进行研究，以1）记录和监测 HIV-1遗传多样性和进化; 2）记录和监测抗逆转录病毒药物耐药性的出现 HIV-1变异体; 3）研究不同HIV-1变异体的生物学和免疫学特性，包括新出现的 和耐药菌株; 4）监测市售检测试剂盒诊断感染的能力， 由不同的HIV-1病毒株引起; 5）提高质量保证和实验室生物安全。 为实现这些目标，我们将： 现场培训：㈠质量保证和实验室生物安全; ㈡新技术和商业 用于诊断各种艾滋病毒株引起的感染的现有检测试剂盒;以及iii）科学和赠款 写作技巧B）在纽约提供长期培训， 生物学/流行病学/生物统计学相关的艾滋病毒遗传多样性的研究，通过非学位或学位 博士前和博士后研究员的计划。c）在纽约为研究员提供中期培训， 进行病毒遗传亚型表征和计算分析的实验室实践培训， 记录和鉴定不同的HIV-1毒株和/或耐药突变，以及病毒生物学和 免疫特性d）提供短期培训，包括药物登记方面的实际操作培训 耐药性突变和数据解释，参加流行病学和生物统计学课程， 与相关导师进行一对一的交流以及，e）通过提供小额赠款支持国内研究， 适当的长期“校友”研究员进行研究，解决MDC的研究目标。这 培训计划应建立和加强医学发展中心开展HIV-1研究的能力 多样性和耐药性与喀麦隆的干预和预防方案有关。

Project Summary /Abstract The broad HIV-1 genetic diversity and the emergence of drug resistant variants in Cameroon adds to complicate the viral genetic diversity and poses potential problems for diagnosis and prevention efforts, requiring that the evolution of HIV-1 diversity be monitored, and that new HIV-1 variants be examined for sensitivity of detection by different HIV-1 test kits. These problems continue to underscore the need for further training and research in Cameroon. This training program entitled "Training Program on HIV Diversity and Drug Resistance: enhancing research capacity" is designed with long-term to build and strengthen research capacity at the Medical Diagnostic Center in Yaounde, Cameroon for conducting studies to 1) document and monitor HIV-1 genetic diversity and evolution; 2) document and monitor the emergence antiretroviral drug resistant HIV-1 variants; 3) study the biologic and immunologic properties of different HIV-1 variants including emerging and drug resistant strains; 4) monitor the ability of commercially available test kits to diagnose infections by caused by diverse HIV-1 strains; and 5) to improve quality assurance and laboratory biosafety. To achieve these objectives we will: a) Organize in-country training workshops that will provide short-term local onsite training on: i) quality assurance and laboratory biosafety; ii) new technologies and commercially available test kits for the diagnosis of infections caused by diverse HIV strains; and iii) scientific and grant writing skills. b) Provide long-term training in New York for studies of molecular biology/epidemiology/biostatistics relevant to studies of HIV genetic diversity through non-degree or degree programs for pre- and post-doctoral fellows. c) Provide medium-term training in New York for fellows to perform hands-on-laboratory training on viral genetic subtype characterization and computational analysis to document and identify diverse HIV-1 strains and/or drug resistance mutations, and viral biologic and immunologic properties. d) Provide short-term training through hands-on-training in documenting drug resistance mutations and data interpretation, to attend courses on epidemiology and biostatistics and to work one-on-one with relevant mentors. And, e) To support in-country research by providing small grants to appropriate long-term "alumni" fellows to conduct studies that addresses the research goals of the MDC. This training program should build and strengthen the research capacity at MDC to conduct studies on HIV-1 diversity and drug resistance that are relevant to intervention and prevention programs in Cameroon.