MEMS Based Intraoperative Photoacoustic Imaging

描述(申请人提供)：保乳手术，或肿块切除术，是诊断为早期浸润性乳腺癌患者最常见的外科手术。明确的肿瘤边缘和完全手术切除肿瘤对于防止局部复发和提高无病生存率至关重要。目前，还没有准确的方法来确定手术前或手术中的肿瘤边缘。大约20%到30%的接受肿块切除术的患者需要额外的手术来切除任何残留的肿瘤，因为存在阳性的肿瘤边缘。为了开发一种灵敏的检测乳腺组织肿瘤边缘的方法，我们提议设计一种肿瘤靶向纳米颗粒成像探针，并利用基于微电子机械系统(MEMS)的光声断层扫描(PAT)实现术中成像。本研究的目标是：1)开发一种用于术中肿瘤成像的基于MEMS的双频光声成像系统；2)利用体模和体内实验来验证和优化纳米探针-MEMS-PAT系统；以及3)评估体内纳米探针-MEMS-PAT引导下切除小鼠乳腺肿瘤的效果。基于微型MEMS的灵敏、高分辨率的术中光声成像系统的发展，在术中确定肿瘤边缘、预防肿瘤复发、提高乳腺癌患者的生存率等方面具有很大的潜力。

DESCRIPTION (provided by applicant): Breast-conserving surgery, or lumpectomy, is the most common surgical procedure for patients diagnosed with an early stage of invasive breast cancer. Clearly defined tumor margins and complete surgical removal of tumors are critical for preventing local recurrence and increasing disease-free survival. Currently, there is no accurate method to identify tumor margins pre- or intra-operatively. About 20% to 30% of the patients who undergo lumpectomy require additional surgery to excise any remaining tumors due to the presence of positive tumor margins. To develop a sensitive approach for detecting tumor margins in breast tissues, we propose to engineer a tumor targeted nanoparticle imaging probe and to realize the intraoperative imaging using microelectromechanical systems (MEMS) based photoacoustic tomography (PAT). The objectives of this proposed study are: 1) To develop a MEMS based dual-frequency photoacoustic tomography system for intraoperative tumor imaging; 2) To validate and optimize the nanoprobe-MEMS-PAT system using phantom and in vivo experiments; and 3) To evaluate the efficacy of in vivo nanoprobe-MEMS-PAT guided resection of mouse mammary tumors. The development of a sensitive and high resolution miniaturized MEMS based intraoperative photoacoustic imaging system should have great potential for determining tumor margins during surgery, preventing tumor recurrence and therefore, increasing the survival rate of breast cancer patients.