权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular Mechanism of Akirin Function During Myogenesis

肌生成过程中 Akirin 功能的分子机制

基本信息

批准号：
8497302
负责人：
Scott James Nowak
金额：
$ 31万
依托单位：
KENNESAW STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-05-01 至 2018-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8497302
关键词：
Adult Animal Model Award Binding Biochemical Biochemistry Biological Assay Biomedical Research Chromatin Remodeling Factor Communities Data Disease Drosophila genus Drosophila melanogaster Embryo Embryology Event Failure Funding Future Gene Expression Gene Expression Regulation Generations Genetic Genetic Transcription Goals Heterozygote Homeostasis In Vitro Injury Insecta Institution Laboratories Learning Link Maintenance Malignant Neoplasms Mammals Mediating Mentors Messenger RNA Molecular Molecular Biology Molecular Genetics Multiprotein Complexes Muscle Muscle Development Muscle Fibers Myoblasts Natural regeneration Nature Neurodegenerative Disorders Nuclear Protein Participant Patients Pattern Play Population Process Protein Isoforms Proteins Recruitment Activity Research Research Personnel Research Project Grants Role Students Techniques Time Training Treatment Efficacy brahma career chromatin remodeling cofactor cost deletion analysis domain mapping experience improved in vivo member myogenesis novel prevent programs public health relevance repaired skeletal skills skills training success transcription factor undergraduate research undergraduate student wasting

项目摘要

DESCRIPTION (provided by applicant): The specification and differentiation of myoblasts by the action of myogenic transcription factors is a key event in the formation of the skeletal musculature. Following embryonic muscle development, the specification and differentiation of myoblasts remains essential for the maintenance and regeneration of healthy adult muscle in mammals. Failure to maintain muscle homeostasis properly through the establishment of new populations of myoblasts leads to progressive degenerative muscle wasting. However, despite the primary importance of myoblast specification and differentiation for myogenesis, the identities of new molecular players in this process remain unknown. Preliminary studies in the fruit fly, Drosophila melanogaster, have recently identified a novel participant in myogenesis, the conserved nuclear protein Akirin. Akirin appears to play an important yet still unidentified role i the regulation of gene expression during myoblast specification. The overall goal of this proposal is to use Drosophila to identify and characterize the molecular mechanism of gene regulation by Akirin during myogenesis. The rationale for the proposed research is that through understanding the modes of action of novel regulators of myogenesis such as Akirin, the efficacy of therapies aimed at mitigating or reversing muscle wasting can be improved. We will study Akirin's mode of action through two specific aims. First, we will perform deletion analysis to identify the domains of interaction between Akirin and the transcriptional machinery that are critical for Akirin-mediated gene regulation. Second, we will use a double heterozygote assay to identify candidate proteins that interact with Akirin during muscle development, and are essential cofactors for Akirin-mediated gene regulation. Critically, in keeping with the goals of the AREA award mechanism, this project will give undergraduate researchers hands-on experience in a wide variety of molecular, genetic and biochemical techniques, which will provide a valuable skill set for a future career in biomedical research.

描述（由申请人提供）：在肌源性转录因子的作用下，成肌细胞的特化和分化是骨骼肌组织形成的关键事件。在胚胎肌肉发育之后，成肌细胞的分化和分化对哺乳动物健康成年肌肉的维持和再生至关重要。不能通过建立新的成肌细胞群来适当地维持肌肉稳态，导致进行性退行性肌肉萎缩。然而，尽管成肌细胞的规范和分化对肌肉形成至关重要，但在这一过程中新的分子参与者的身份仍然未知。在果蝇（Drosophila melanogaster）的初步研究中，最近发现了一种肌肉形成的新参与者