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Long Non-coding RNAs in Adipogenesis

脂肪形成中的长非编码RNA

基本信息

批准号：
8716868
负责人：
BIN TIAN
金额：
$ 2.6万
依托单位：
RBHS-NEW JERSEY MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-12-05 至 2013-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8716868
关键词：
3T3-L1 Cells Accounting Adipocytes Affect Area Bioinformatics Biological Assay Biological Markers Cardiovascular Diseases Cell Count Cell Differentiation process Cell Line Cell Nucleus Cell Proliferation Cells Code Computing Methodologies Data Data Set Development Diabetes Mellitus Disease Epigenetic Process Event Expressed Sequence Tags Foundations Functional RNA Future Gene Expression Profile Gene Expression Regulation Genes Genetic Transcription Genome Genomics Grant Human Human Genome Light Mammals Mesenchymal Stem Cells Methods Molecular Molecular Biology Mus Non-Insulin-Dependent Diabetes Mellitus Northern Blotting Obesity Play Process Proteins Public Health RNA RNA Processing RNA analysis Regulation Reporting Research Role Signal Transduction Solid Staging Structure Time Transcript Untranslated RNA Validation chromatin remodeling deep sequencing experience genome-wide human disease knock-down lipid biosynthesis mammalian genome mouse genome novel precursor cell transcriptome sequencing

项目摘要

DESCRIPTION (provided by applicant): The mammalian genomes express a large number of long noncoding RNAs (lncRNAs). Only a handful of them, however, have been studied. The known lncRNAs have diverse roles in the cell, mostly in the nucleus, such as chromatin remodeling, transcription, and RNA processing. Adipogenesis is the process that generates adipocytes from precursor cells, which have many implications for human diseases, such as diabetes and cardiovascular diseases. While regulation of protein-coding genes in adipogenesis has been well studied, the role of lncRNAs is completely unclear. We have sequenced the transcriptomes of human and mouse cells undergoing adipogenesis to an unprecedented depth. Our preliminary analysis indicates that a set of lncRNAs are highly regulated during adipogenesis, suggesting their functional roles in the process. In this grant, we plan to examine known and novel lncRNAs that are regulated in adipogenesis for their roles in differentiation of precursor cells into mature adipocytes.

描述（由申请人提供）：哺乳动物基因组表达大量长非编码RNA（lncRNA）。然而，只有其中的一小部分得到了研究。已知的lncRNA在细胞中具有多种作用，主要是在细胞核中，例如染色质重塑、转录和RNA加工。脂肪生成是从前体细胞产生脂肪细胞的过程，这对人类疾病有很多影响，例如糖尿病和心血管疾病。虽然脂肪生成中蛋白质编码基因的调控已得到充分研究，但 lncRNA 的作用尚不清楚。我们对经历脂肪形成的人类和小鼠细胞的转录组进行了前所未有的深度测序。我们的初步分析表明，一组 lncRNA 在脂肪形成过程中受到高度调控，表明它们在此过程中的功能作用。在这笔资助中，我们计划检查在脂肪生成中受到调节的已知和新型 lncRNA 在前体细胞分化为成熟脂肪细胞中的作用。