Regulation of Alternative Cleavage and Polyadenylation

在此输入文本，它是您的应用程序的新摘要信息。此部分必须为no 超过30行的文本。 前mRNA裂解/多聚腺苷化(C/P)定义了成熟转录本的3‘端。过半 的人类基因具有多个C/P位点(PA)，导致不同的mRNA亚型不同 编码序列(CDS)和/或3‘非翻译区(3’UTRs)。备用PA选择 (APA)迅速被认为是影响蛋白质多样性的重要基因调节层 和信使核糖核酸的代谢。基因的APA模式因细胞/组织类型的不同而不同 在不同的条件下动态调节，如发展和分化。这个 然而，对APA的作用机制知之甚少。我们的长期目标是了解 APA在细胞/组织类型以及发育和分化过程中受到调节。在这笔赠款中， 我们有三个特定目标(SA)：SA-1，用于分析不同细胞类型和 使用新转录的RNA；SA-2进行分化，以检测RNA结合蛋白在 C/P复合体参与了APA；SA-3，以考察C/P因素在 启动子影响APA。我们预计这笔拨款的结果将提供关于 并阐明其在细胞功能和分化中的意义。

Enter the text here that is the new abstract information for your application. This section must be no longer than 30 lines of text. Pre-mRNA cleavage/polyadenylation (C/P) defines the 3' end of a mature transcript. Over half of the human genes have multiple C/P sites (pAs), resulting in mRNA isoforms with different coding sequences (CDS) and/or 3' untranslated regions (3'UTRs). Alternative pA selection (APA) is rapidly recognized as an important layer of gene regulation, affecting protein diversity and mRNA metabolism. The APA pattern of genes varies across cell/tissue types and is dynamically regulated under different conditions, such as development and differentiation. The mechanisms of APA, however, are poorly understood. Our long-term goal is to understand how APA is regulated across cell/tissue types and in development and differentiation. In this grant, we have three Specific Aims (SAs): SA-1, to analyze APA regulation across cell types and in differentiation using newly transcribed RNA; SA-2, to examine how RNA-binding proteins in the C/P complex are involved in APA; SA-3, to examine how recruitment of C/P factors at the promoter affects APA. We expect the results of this grant will provide systematic views on the mechanisms of APA and elucidate its significance in cell functions and in differentiation.