High Speed 10-Color Flow Cytometer

高速 10 色流式细胞仪

• 批准号: 8247492
• 负责人: Debra L Laskin
• 金额: $ 22.24万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247492
• 关键词: 9 year old; Asthma; Biomedical Research; Cells; Color; Core Facility; Cytometry; Dentistry; Diabetes Mellitus; Disease; Dyes; Flow Cytometry; Funding; Green Fluorescent Proteins; Image Analysis; Joints; Malignant Neoplasms; Medicine; Monoclonal Antibodies; New Jersey; Population; Research; Research Personnel; Services; Speed; United States National Institutes of Health; Universities; Wood material; instrument; medical schools; novel

DESCRIPTION (provided by applicant): The application of high speed multiparameter flow cytometry has revolutionized many aspects of biomedical research. Paired with the use of monoclonal antibodies, green fluorescent proteins, and other fluorescent indicator dyes, cell subsets have been identified and characterized that have led to novel treatments for diseases such as cancer, asthma, and diabetes. A group of 33 NIH-supported researchers at Rutgers University and the University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School are requesting funds to purchase a Beckman Coulter Gallios flow cytometer to replace a 9 year old cytometer in the joint University's Shared Analytical Cytometry/Image Analysis Core Facility. The instrument chosen will provide the critical capability of analyzing rar cell populations at rapid rates with exceptionally high sensitivity and stability. The majority of he users have previously used services of the Core facility for their research. The requested instrument will expand their capabilities and increase the pace of NIH supported scientific research.

描述（由申请人提供）：高速多参数流式细胞术的应用彻底改变了生物医学研究的许多方面。与单克隆抗体、绿色荧光蛋白和其他荧光指示剂染料的使用配对，细胞亚群已经被鉴定和表征，这导致了对诸如癌症、哮喘和糖尿病的疾病的新治疗。罗格斯大学和新泽西州医学和牙科大学罗伯特伍德约翰逊医学院的33名NIH支持的研究人员正在申请资金购买Beckman Coulter Gallios流式细胞仪，以取代联合大学共享分析细胞术/图像分析核心设施中的9岁细胞仪。选择的仪器将提供快速分析rar细胞群的关键能力，具有极高的灵敏度和稳定性。大多数用户以前都使用过核心设施的服务进行研究。所要求的工具将扩大他们的能力，并加快NIH支持的科学研究的步伐。

项目成果

Simultaneous determination of 3-mercaptopyruvate and cobinamide in plasma by liquid chromatography-tandem mass spectrometry.

液相色谱-串联质谱法同时测定血浆中3-巯基丙酮酸和可滨酰胺。

• DOI: 10.1016/j.jchromb.2015.11.027
• 发表时间: 2016
• 期刊: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
• 作者: Stutelberg,MichaelW;Dzisam,JosephK;Monteil,AlexandreR;Petrikovics,Ilona;Boss,GerryR;Patterson,StevenE;Rockwood,GaryA;Logue,BrianA
• 通讯作者: Logue,BrianA