Functional Testing Core (FTC)

功能测试核心 (FTC)

• 批准号: 8380324
• 负责人: KEVIN K. OHLEMILLER
• 金额: $ 18.15万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-26 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8380324
• 关键词: Advertising; Animal Model; Animals; Auditory; Auditory Brainstem Responses; Brain Stem; Collaborations; Communities; Data; Diagnosis; Discipline; Electroretinography; Elements; Equipment; Eye Movements; Face; Frequencies; Functional disorder; Genes; Goals; Health; Instruction; Intranet; Labyrinth; Light; Masks; Measures; Methods; Modeling; Molecular; Motor; Mus; Neurons; Outer Hair Cells; Output; Pathology; Public Health; Publishing; Recruitment Activity; Reporting; Research; Research Personnel; Rodent; Rodent Model; Rotation; Scientist; Seeds; Sensory; Services; Technology; Testing; Transgenic Organisms; Vision; Visual; Visual system structure; experience; insight; interest; investigator training; knockout gene; member; middle ear disorder; otoacoustic emission; relating to nervous system; response; virtual

The advent of transgenic technology in rodents has yielded a goldmine of models offering insights into molecular and cellular aspects of sensory function and pathology. The vibrant research community at WUSM includes many investigators with a primary interest in sensory function and dysfunction. Moreover, because insights sometimes come from models intended for other purposes, all investigators must be encouraged to approach their models from a broad perspective, and should face as few roadblocks as possible in asking larger questions. The goal of the RCAVS Functional Testing Core (FTC) is to impart to investigators the ability to assess the auditory, vestibular, and visual systems of small animals in a uniform way, applying state-of-the-art methods and equipment. Accordingly, the FTC maintains and operates equipment serving the separate requirements of vestibular, auditory, and visual testing to make well conceived, comprehensive functional testing available to our colleagues within the Research Core Center and the larger research community. The Specific Aims of the FTC are: 1) To facilitate comprehensive sensory (inner ear and visual) testing in mouse and other small animal models. Auditory tests offered include auditory brainstem response (ABR) thresholds, ABR input/output analyses, ABR waveform and latency analysis, and ABR temporal and spectral masking profiles. In addition, distortion product otoacoustic emissions'(DPOAEs) provide information about outer hair cell motor function. Vestibular tests principally encompass tracking of eye movements through analysis of reflected light as animals are subjected to controlled rotations. Finally, our experience has shown that inner ear and visual pathology often coincide. We have published expertise in recording of flash electroretinograms (ERGs) and have incorporated this capability into our equipment and testing regime. All tests can either be done by Core staff, or we can train an investigator's own staff for perform tests. 2) To provide consultative services that support proper application of sensory tests and reporting of results. Upon request. Core staff assist investigators in interpretation of findings, statistical analyses, and presentation conventions. 3) To promote interactions and stimulate new research endeavors by RCAVS investigators and throughout WUSM. Research Core services are advertised on the WUSM intranet, and promoted in occasional forums. The RCAVS has sparked collaborations between members of several departments and continues to seed interactions between scientists in diverse disciplines.

转基因技术在啮齿动物中的出现产生了一个金矿模型，提供了对感觉功能和病理学的分子和细胞方面的见解。WUSM充满活力的研究社区包括许多对感觉功能和功能障碍感兴趣的研究人员。此外，由于洞察力有时来自于用于其他目的的模型，因此必须鼓励所有研究人员从广泛的角度来研究他们的模型，并且在提出更大的问题时应该尽可能少地遇到障碍。RCAVS功能测试核心（FTC）的目标是赋予研究人员以统一的方式评估小动物的听觉，前庭和视觉系统的能力，应用最先进的方法和设备。因此，联邦贸易委员会维护和操作设备服务前庭，听觉和视觉测试的单独要求，使精心构思，全面的功能测试提供给我们的同事在研究核心中心和更大的研究社区。FTC的具体目标是：1）促进在小鼠和其他小动物模型中进行全面的感觉（内耳和视觉）测试。提供的听觉测试包括听觉脑干反应（ABR）阈值，ABR输入/输出分析，ABR波形和潜伏期分析，以及ABR时间和频谱掩蔽曲线。此外，畸变产物耳声发射（DPOAEs）提供有关外毛细胞运动功能的信息。前庭测试主要包括通过分析反射光跟踪眼球运动，因为动物受到控制旋转。最后，我们的经验表明，内耳和视觉病理往往是一致的。我们已经发表了记录闪光视网膜电图（ERG）的专业知识，并将这种能力纳入我们的设备和测试制度。所有测试都可以由核心人员完成，或者我们可以培训研究人员自己的工作人员进行测试。2)提供咨询服务，支持正确应用感官测试和报告结果。应要求核心工作人员协助调查人员解释调查结果、统计分析和介绍惯例。3)促进互动，并通过RCAVS研究人员和整个WUSM刺激新的研究努力。研究核心服务在WUSM内联网上做广告，并在偶尔的论坛上推广。RCAVS引发了几个部门的成员之间的合作，并继续在不同学科的科学家之间的种子互动。